Inhibitory Effect of GRAS Essential Oils and Plant Extracts on the Growth of Aspergillus westerdijkiae and Aspergillus carbonarius Strains

Abstract

The effect of essential oils (obtained using hydrodistillation) and plant extracts (ethanolic, aqueous, and hexanic extractions) of 10 different plants cultivated in Brazil were tested using the diffusion agar method, with the objective of evaluating the inhibitory effect of the oils and extracts on the mycelial growth of Aspergillus westerdijkiae NRRL 3174 and A. carbonarius RC 2054 (UNRC). Of the 40 essential oils and plant extracts analyzed, oregano essential oil and plant extract, rosemary essential oil, and the clove ethanolic extract were the best choice to obtain the growth parameters (radial growth rates (mm day−1) and lag phase (h)) due the good results presented and the volume of oil/extract obtained. Comparing all the essential oils and plant extracts that were tested for growth parameters, the best results were obtained for the clove ethanolic extract for both strains assayed. These results demonstrated an outstanding potential use of some of these products in prevention of fungal contamination in food. However, further studies need to be conducted to determine the ability of these oils and extracts to inhibit or reduce ochratoxin A production.

Characterization and Inhibitory Effects of Essential Oil and Nanoemulsion from Ocotea indecora (Shott) Mez in Aspergillus Species

“…The comparison was even wider for the A. westerdjikiae strain, as all concentrations showed significant activity in the first 48 h, and the higher ones up to 96 h. The greater susceptibility of A. westerdjikiae to bioproducts was already reported in other studies, such as those of Rodrigues et al [44] and Schlösser and Prange [45].…”

Produção de beta-D-frutofuranosidases por Aspergillus sp. M2.4 e caracterização bioquímica

“…O perfil de crescimento dos micro-organismos, inicia-se com a ambientação da cepa ao meio de cultivo (fase lag), seguida pela fase log, que é o crescimento exponencial, acompanhado do consumo dos nutrientes disponíveis e liberação de metabólitos primários, como as enzimas. Por fim, há as etapas de decréscimo, que representa a morte da cepa e a produção de metabólitos secundários, o que reprime a as enzimas extracelulares (Jeennor et al, 2022;Rodrigues et al, 2022). Foi relatado que no terceiro dia de cultivo de S. cerevisiae, nas condições ótimas, produziu-se invertases com atividade de 1.73 U mL -1 , enquanto no quinto dia houve um dos menores valores expressos (1.2 U mL -1 ) (Mahendran et al, 2022).…”