Inhibitory Effect of Alcohol on Osteogenic Differentiation in Human Bone Marrow–Derived Mesenchymal Stem Cells

Gong, Zhaodi; Wezeman, Frederick H.

doi:10.1097/01.alc.0000118315.58404.c1

Cited by 71 publications

(59 citation statements)

References 59 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Alcohol causes growth retardation, a decrease in animal weight and weight gain inversely proportional to the dosage of alcohol (Gong and Wezeman, 2004).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

The action of demineralized bovine bone matrix on bone neoformation in rats submitted to experimental alcoholism

Buchaim

Andreo

Rodrigues

et al. 2013

Arq. Bras. Med. Vet. Zootec.

View full text Add to dashboard Cite

The action of demineralized bovine bone matrix on bone neoformation in rats submitted to experimental alcoholism ABSTRACTThe objective of this study was to evaluate whether demineralized bovine bone (Gen-ox ® ) alters bone neoformation in rats submitted to alcoholism. Forty male rats were separated into two groups of 20 rats and distributed as follows: Group E1, which received 25% ethanol and a surgical cavity filled only by a blood clot, and Group E2, which received 25% ethanol and a surgical cavity filled with Gen-ox ® . The animals were euthanized at 10, 20, 40 and 60 days after surgery and necropsy was performed. The histomorphological and histometric analyses of the area of connective tissue and bone neoformation showed that the reorganization of the bone marrow and full repair of the surgical cavity in Group E1 occurred more quickly than in Group E2. It was also noted that in the final period the animals in Group E2 showed areas of connective tissue and thick bone trabeculae around the particles of the implant. It can be concluded that the use of Gen-ox ® delayed the process of bone repair in alcoholic rats, although it can be used as filling material because it shows osteoconductive activity, as evidenced by bone tissue formation around the graft particles.

show abstract

“…Alcohol causes growth retardation, a decrease in animal weight and weight gain inversely proportional to the dosage of alcohol (Gong and Wezeman, 2004).…”

Section: Discussionmentioning

confidence: 99%

“…Acute ingestion of alcohol causes an inhibitory effect on the parathyroid and also on osteoblastic cells, contributing to the development of bone disease (Garcia-Sanchez et al, 1995;Gong and Wezeman, 2004;Iwaniec et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

The action of demineralized bovine bone matrix on bone neoformation in rats submitted to experimental alcoholism

Buchaim

Andreo

Rodrigues

et al. 2013

Arq. Bras. Med. Vet. Zootec.

View full text Add to dashboard Cite

show abstract

“…Ethanol has been shown to inhibit osteoblast proliferation and function in vitro [18] and thus prenatal ethanol exposure may affect fetal bone development through direct effects on osteoblasts. Moreover, chronic ethanol exposure in the adult results in increased total bone and bone marrow fat content in both humans and animals [7 and 38], and ethanol has been shown to inhibit osteogenic differentiation [7] and increase adipogenesis [38] in mesenchymal stem cells. Interestingly, preliminary data indicate that the tibia of fetal guinea pigs prenatally exposed to ethanol also has increased fat content (Keiver and Brien, unpublished data), thereby suggesting that ethanol also alters the balance between adipogenesis and osteogenesis in the fetus.…”

Section: Discussionmentioning

confidence: 99%

Prenatal ethanol exposure disrupts the histological stages of fetal bone development

Snow

Keiver

2007

Bone

View full text Add to dashboard Cite

Maternal ethanol intake during pregnancy results in impairments in general growth and skeletal development in the offspring. We have previously shown that ethanol retards skeletal ossification at doses lower than those that affect growth. Moreover skeletal sites vary in their sensitivity to ethanol effects, with more severe effects occurring in bones that undergo a greater proportion of their development in utero. Taken together, these data suggest that ethanol has specific effects on bone development, and that later stages in the ossification process may be particularly affected. Such effects could have important implications for the offspring's long-term bone health, as studies suggest that the intrauterine environment can program the skeleton. The present study examined the histological stages of bone development to determine if prenatal ethanol exposure alters the morphological development of the growth plate in the fetal rat. Rats were fed a liquid diet containing ethanol (Ethanol, E group), or without ethanol (Pair-Fed, PF, or Control, C groups) for 6 weeks: 3 weeks prior to breeding and during 3 weeks of pregnancy. Fetal tibiae were fixed, decalcified and stained for histological analysis on day 21 of gestation. Maternal ethanol intake resulted in a significant decrease in fetal total bone and diaphysis lengths, compared with tibiae from PF and C fetuses. Although the lengths of the epiphyses were not affected, ethanol disrupted the organization of the histological zones within the epiphyses. Prenatal ethanol exposure decreased the length of the resting zone, but increased the length of the hypertrophic zone. Enlargement of the hypertrophic zone is consistent with an effect of ethanol on the later stages of bone development; however ethanol's effect on the resting zone indicate that earlier stages of bone development may also be disrupted. The functional significance of these morphological changes to long-term bone health remains to be determined.

show abstract

“…58 Some other studies regard type I collagen as an early marker and ALP as a middle marker of osteogenesis. 59 Osteocalcin (also known as bone gamma-carboxyglutamic acid-containing protein) is a noncollagenous calcium-binding bone protein referred to as a late osteogenic marker. 58 The expression of type I collagen, ALP, and osteocalcin genes under differentiation conditions with AA addition did not differ on NCD films with different wettability or on the control polystyrene dish.…”

mentioning

confidence: 99%

Osteogenic cell differentiation on H-terminated and O-terminated nanocrystalline diamond films

Lišková¹,

Babchenko²,

Varga³

et al. 2015

IJN

View full text Add to dashboard Cite

Nanocrystalline diamond (NCD) films are promising materials for bone implant coatings because of their biocompatibility, chemical resistance, and mechanical hardness. Moreover, NCD wettability can be tailored by grafting specific atoms. The NCD films used in this study were grown on silicon substrates by microwave plasma-enhanced chemical vapor deposition and grafted by hydrogen atoms (H-termination) or oxygen atoms (O-termination). Human osteoblast-like Saos-2 cells were used for biological studies on H-terminated and O-terminated NCD films. The adhesion, growth, and subsequent differentiation of the osteoblasts on NCD films were examined, and the extracellular matrix production and composition were quantified. The osteoblasts that had been cultivated on the O-terminated NCD films exhibited a higher growth rate than those grown on the H-terminated NCD films. The mature collagen fibers were detected in Saos-2 cells on both the H-terminated and O-terminated NCD films; however, the quantity of total collagen in the extracellular matrix was higher on the O-terminated NCD films, as were the amounts of calcium deposition and alkaline phosphatase activity. Nevertheless, the expression of genes for osteogenic markers – type I collagen, alkaline phosphatase, and osteocalcin – was either comparable on the H-terminated and O-terminated films or even lower on the O-terminated films. In conclusion, the higher wettability of the O-terminated NCD films is promising for adhesion and growth of osteoblasts. In addition, the O-terminated surface also seems to support the deposition of extracellular matrix proteins and extracellular matrix mineralization, and this is promising for better osteoconductivity of potential bone implant coatings.

show abstract

Inhibitory Effect of Alcohol on Osteogenic Differentiation in Human Bone Marrow–Derived Mesenchymal Stem Cells

Abstract: Collectively, the data suggest that alcohol alters osteogenic differentiation in human bone marrow-derived mesenchymal stem cell cultures during lineage progression and provide further insight into alcohol-induced reduced bone formation.

Cited by 71 publications

References 59 publications

The action of demineralized bovine bone matrix on bone neoformation in rats submitted to experimental alcoholism

The action of demineralized bovine bone matrix on bone neoformation in rats submitted to experimental alcoholism

Prenatal ethanol exposure disrupts the histological stages of fetal bone development

Osteogenic cell differentiation on H-terminated and O-terminated nanocrystalline diamond films

Contact Info

Product

Resources

About