The action of demineralized bovine bone matrix on bone neoformation in rats submitted to experimental alcoholism

Abstract

The action of demineralized bovine bone matrix on bone neoformation in rats submitted to experimental alcoholism ABSTRACTThe objective of this study was to evaluate whether demineralized bovine bone (Gen-ox ® ) alters bone neoformation in rats submitted to alcoholism. Forty male rats were separated into two groups of 20 rats and distributed as follows: Group E1, which received 25% ethanol and a surgical cavity filled only by a blood clot, and Group E2, which received 25% ethanol and a surgical cavity filled with Gen-ox ® . The animals were euthanized at 10, 20, 40 and 60 days after surgery and necropsy was performed. The histomorphological and histometric analyses of the area of connective tissue and bone neoformation showed that the reorganization of the bone marrow and full repair of the surgical cavity in Group E1 occurred more quickly than in Group E2. It was also noted that in the final period the animals in Group E2 showed areas of connective tissue and thick bone trabeculae around the particles of the implant. It can be concluded that the use of Gen-ox ® delayed the process of bone repair in alcoholic rats, although it can be used as filling material because it shows osteoconductive activity, as evidenced by bone tissue formation around the graft particles.

Biological Behavior of Xenogenic Scaffolds in Alcohol-Induced Rats: Histomorphometric and Picrosirius Red Staining Analysis

“…Alcohol interfered negatively in bone neoformation, presenting a greater delay in the entire process. The literature agrees with the results obtained as it points out that the chronic use of alcohol inhibits and delays bone formation [10,24,[30][31][32].…”

“…The animals received the same solid diet "ad libitum" throughout the experiment (Nuvilab ® , Nuvital, Colombo, Brazil). The rats from Groups AG/B and AG/O were submitted to gradual adaptation to alcohol (absolute ethyl alcohol, Synth ® , Diadema, Brazil), to induce chronic alcoholism and to prevent its death [24,25], being 7 days of an alcoholic diet at 8% (v/v), 7 days with alcohol at 16% (v/v) and 7 days at 25% (v/v), remaining in this last concentration until the end of the experiment (euthanasia).…”

Action of a deproteinized xenogenic biomaterial in the process of bone repair in rats submitted to inhalation of cigarette smoke

“…In the histomorphological analysis, the formation of a new cortex and the reorganization of the medullar region were observed in the group in which the surgical cavity was filled only by clot (CCG). This finding is expected in the bone repair process in the model used, even with the presence of aggravating factors such as exposure to ethanol or nicotine 6,7,23 . Smoking leads to decreased bone mineral density, including the risk of osteoporosis in postmenopausal women 24,25 .…”

“…The use of the GenOx biomaterial led to the formation of tissue with characteristics similar to an osteoid matrix in the postoperative period of 6 months, according to da Silva et al 20 . In addition, when associated with animals submitted to chronic alcoholism, despite not accelerating the process of bone reconstitution, the demineralized bone matrix presented osteoconductive properties, with formation of new bone tissue aggregating the biomaterial particles 6 .…”

“…Nicotine does not have a direct pharmacological effect on bone density but has an indirect effect on bone mineral density decrease and is an inducing factor in the occurrence of osteoporosis, aggravated if it is associated with chronic alcoholism [5][6][7] .…”