Inhibitory Effect of Additives on Cellulase Adsorption Mediated by Hydrophobic Interaction

Ueno, Yoshiki; Taneda, Daisuke; Okino, Shohei; Shirai, Yoshihito

doi:10.1627/jpi.61.357

Cited by 2 publications

(3 citation statements)

References 16 publications

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“…Numerous studies have referred hydrophobic interaction as the primary factor governing the adsorption between protein and lignin. Researchers (Ueno et al 2018 ) reported that the cellulase molecules could be significantly adsorbed by hydrophobic but uncharged graphite in enzymatic buffer solution, which highly inhibited the hydrolysis of filter paper. This inhibition was effectively overcome with the addition of BSA while PEG had no promotion effect.…”

Section: Resultsmentioning

confidence: 99%

Understanding the promoting effect of non-catalytic protein on enzymatic hydrolysis efficiency of lignocelluloses

Gong

Yang

Song

et al. 2021

Bioresour. Bioprocess.

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Lignin deposits formed on the surface of pretreated lignocellulosic substrates during acidic pretreatments can non-productively adsorb costly enzymes and thereby influence the enzymatic hydrolysis efficiency of cellulose. In this article, peanut protein (PP), a biocompatible non-catalytic protein, was separated from defatted peanut flour (DPF) as a lignin blocking additive to overcome this adverse effect. With the addition of 2.5 g/L PP in enzymatic hydrolysis medium, the glucose yield of the bamboo substrate pretreated by phenylsulfonic acid (PSA) significantly increased from 38 to 94% at a low cellulase loading of 5 FPU/g glucan while achieving a similar glucose yield required a cellulase loading of 17.5 FPU/g glucan without PP addition. Similar promotion effects were also observed on the n-pentanol-pretreated bamboo and PSA-pretreated eucalyptus substrates. The promoting effect of PP on enzymatic hydrolysis was ascribed to blocking lignin deposits via hydrophobic and/or hydrogen-bonding interactions, which significantly reduced the non-productive adsorption of cellulase onto PSA lignin. Meanwhile, PP extraction also facilitated the utilization of residual DPF as the adhesive for producing plywood as compared to that without protein pre-extraction. This scheme provides a sustainable and viable way to improve the value of woody and agriculture biomass. Peanut protein, a biocompatible non-catalytic protein, can block lignin, improve enzymatic hydrolysis efficiency and thereby facilitate the economics of biorefinery. Graphical abstract

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Section: Resultsmentioning

confidence: 99%

Understanding the promoting effect of non-catalytic protein on enzymatic hydrolysis efficiency of lignocelluloses

Gong

Yang

Song

et al. 2021

Bioresour. Bioprocess.

View full text Add to dashboard Cite

show abstract

“…Its hydrophilic group exerts a steric hindrance effect on cellulase to prevent the hydrophobic part of lignin from interacting with cellulase. Tween 80 can improve the adsorption and desorption performance of cellulose [56]. It can reduce the ineffective adsorption of endoglucanase and allow free endoglucanase to produce more free chain end-groups.…”

Section: Enzymatic Hydrolysismentioning

confidence: 99%

“…Tween 80 can improve the adsorption and desorption performance of cellulose [ 56 ]. It can reduce the ineffective adsorption of endoglucanase and allow free endoglucanase to produce more free chain end-groups.…”

Section: Introductionmentioning

confidence: 99%

Hydrolysis of lignocellulose to succinic acid: a review of treatment methods and succinic acid applications

Zhou

Zhang

Zhu

et al. 2023

Biotechnol Biofuels

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Succinic acid (SA) is an intermediate product of the tricarboxylic acid cycle (TCA) and is one of the most significant platform chemicals for the production of various derivatives with high added value. Due to the depletion of fossil raw materials and the demand for eco-friendly energy sources, SA biosynthesis from renewable energy sources is gaining attention for its environmental friendliness. This review comprehensively analyzes strategies for the bioconversion of lignocellulose to SA based on the lignocellulose pretreatment processes and cellulose hydrolysis and fermentation principles and highlights the research progress on acid production and SA utilization under different microbial culture conditions. In addition, the fermentation efficiency of different microbial strains for the production of SA and the main challenges were analyzed. The future application directions of SA derivatives were pointed out. It is expected that this research will provide a reference for the optimization of SA production from lignocellulose.

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Inhibitory Effect of Additives on Cellulase Adsorption Mediated by Hydrophobic Interaction

Cited by 2 publications

References 16 publications

Understanding the promoting effect of non-catalytic protein on enzymatic hydrolysis efficiency of lignocelluloses

Understanding the promoting effect of non-catalytic protein on enzymatic hydrolysis efficiency of lignocelluloses

Hydrolysis of lignocellulose to succinic acid: a review of treatment methods and succinic acid applications

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