Inhibitory Capacity of Xanthine Oxidase in Antigout Therapy by Indonesian Medicinal Plants

Sianipar, Rut Novalia Rahmawati; Sutriah, Komar; Iswantini, Dyah; Achmadi, Suminar Setiati

doi:10.5530/pj.2022.14.60

Cited by 3 publications

(3 citation statements)

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“…The highest peak on the chromatogram of LC-MS/MS, also indicating the presence of phenolics and the similarity of 17 compounds between the two fractions (Table 2) strongly suggest that these compounds function as competitive inhibitors of XO. These findings are consistent with Sianipar et al (2022) study that flavonoids predominated in 25 Indonesian medicinal plants used as XO inhibitors. This identification's outcomes are also in line with the earlier phytochemical screening (more flavonoid compounds were contained in 70% ethanol extract than water extract) (Rahminiwati et al 2023).…”

Section: Discussionsupporting

confidence: 90%

Antigout Activity of The Spatholobus littoralis Hassk. Extract Fractions Against Xanthine Oxidase: Its Metabolite Profile and Inhibition Kinetics

Sianipar,

Sutriah,

Iswantini

et al. 2023

HAYATI J Biosci

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The Spatholobus littoralis Hassk. (S. littoralis Hassk.) is a native plant in Indonesia and has been widely used in traditional Dayaknese medicine for non-communicable degenerative diseases. One of these illnesses, known as gout, is caused by excessive uric acid in the blood, which is the catalytic byproduct of a xanthine oxidase (XO) enzyme. In this work, we investigated the inhibition kinetics of XO and identified bioactive compounds from the stem extract fractions of S. littoralis Hassk. using liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). Fractionation was carried out to obtain n-hexane, dichloromethane, and 1-butanol fractions from the water and 70% ethanol extracts. Fraction of 1-butanol from 70% ethanol and water extract displayed potent inhibitors of XO with IC50 value 116.91±3.51 and 137.15±5.00 mg/L, respectively. Lineweaver-Burk plot analysis showed that the 1-butanol fraction from the two extracts inhibited XO competitively. The 1-butanol fraction from the two extracts has been further identified as a bioactive fraction. The majority of the compounds in the two active fractions were phenolics. These findings revealed that the 1-butanol fraction from the two extracts is promising as an antigout treatment in the future.

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Section: Discussionsupporting

confidence: 90%

Antigout Activity of The Spatholobus littoralis Hassk. Extract Fractions Against Xanthine Oxidase: Its Metabolite Profile and Inhibition Kinetics

Sianipar,

Sutriah,

Iswantini

et al. 2023

HAYATI J Biosci

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“…63 Flavonoids that lead to competitive inhibitors of XO are isoflavones (genistein), flavonols (kaempferol), flavones (luteolin, apigenin), flavanones (eriodictyol), and flavanols (catechins). 14 These flavonoid compounds are thought to be contained in the water and 70% ethanol extracts from the stem of S. littoralis Hassk.…”

Section: Optimum Conditions For Xo Activitymentioning

confidence: 99%

“…13 The five Indonesian medicinal herbs, Phaleria macrocarpa, Ruellia tuberosa, Sida rhombifolia, Cyperus rotundus, and Syzygium polyanthum, exhibit competitive inhibition kinetics against XO. 14 Hasskarl discovered bajakah tampala in 1842; its scientific name is Spatholobus littoralis Hassk. (S. littoralis Hassk.).…”

Section: Introductionmentioning

confidence: 99%

Optimization of Xanthine Oxidase Activity, Phytochemical Screening, Toxicity Assay, and Antigout Activity of Spatholobus littoralis Hassk. Extract

Rahminiwati¹,

Sianipar²,

Sutriah³

et al. 2023

Pharmacogn J.

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Introduction:A medicinal plant from Central Kalimantan, Spatholobus littoralis Hassk., with a local name bajakah tampala, has long been used to treat gout or uric acid disease. This study aimed to develop the optimum conditions of xanthine oxidase (XO) activity, phytochemical screening, and to obtain the antigout activity of S. littoralis Hassk. Methods: Before performing the phytochemical screening, we performed a simplicia maceration with water and 70% ethanol solvents. The development of the optimum conditions for XO was carried out using the Response Surface Methodology (RSM) combined Box-Behnken Design (BBD). Toxicity assay (LC 50 ) on both extracts was assessed by BSLT (Brine Shrimp Lethality Test). The two extracts were then evaluated for antigout activity. Results: The yield of 70% ethanol extract (11.24%) was higher than the aqueous extract (7.29%). The phytochemical screening on the simplicia and the two extracts positively contained steroids, flavonoids, saponins, and tannins. The optimum conditions of XO activity were temperature of 20 ℃, pH of 8.0, and xanthine concentration of 1.40 mM. The toxicity of the 70% ethanol extract (LC 50 662.25 mg/L) was stronger than the aqueous extract (LC 50 774.68 mg/L). Moreover, the 70% ethanol extract (IC 50 224.14±8.62 mg/L) showed higher XO inhibition than the water extract (IC 50 348.83±4.85 mg/L). Lineweaver-Burk plot analysis showed that 70% ethanol and water extracts inhibited XO competitively with the inhibition constant (K I ) of 272.68 mg/L and 378.44 mg/L, respectively. Conclusion: This preliminary study can be a reference that S. littoralis Hassk. is potentially an antigout.

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The role of selected flavonoids from bajakah tampala (Spatholobus littoralis Hassk.) stem on cosmetic properties: A review

Sianipar

Suryanegara

Fatriasari

et al. 2023

Saudi Pharmaceutical Journal

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Inhibitory Capacity of Xanthine Oxidase in Antigout Therapy by Indonesian Medicinal Plants

Cited by 3 publications

References 0 publications

Antigout Activity of The Spatholobus littoralis Hassk. Extract Fractions Against Xanthine Oxidase: Its Metabolite Profile and Inhibition Kinetics

Antigout Activity of The Spatholobus littoralis Hassk. Extract Fractions Against Xanthine Oxidase: Its Metabolite Profile and Inhibition Kinetics

Optimization of Xanthine Oxidase Activity, Phytochemical Screening, Toxicity Assay, and Antigout Activity of Spatholobus littoralis Hassk. Extract

The role of selected flavonoids from bajakah tampala (Spatholobus littoralis Hassk.) stem on cosmetic properties: A review

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