Inhibitory Activity of Essential Oils of Mentha spicata and Eucalyptus globulus on Biofilms of Streptococcus mutans in an In Vitro Model

Landeo-Villanueva, Guillermo Ernesto; Salazar-Salvatierra, María Elena; Ruiz-Quiroz, Julio Reynaldo; Arriola, Noemí Zuta; Jarama-Soto, Benjamín; Herrera-Calderon, Oscar; Loyola-Gonzales, Eddie

doi:10.3390/antibiotics12020369

Cited by 7 publications

(4 citation statements)

References 41 publications

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“…Recently, essential oils from Eucalyptus globulus with 1,8-Cineol as the main metabolite (65.83%) showed anti-microbial activity against Streptococcus mutans even in biofilm cultures, emulating dental plaque conditions [ 41 ]. Biofilm formation is a major limiting factor concerning the efficacy of anti-microbial treatment due to low penetrability.…”

Section: Anti-microbial and Anti-viral Potentialmentioning

confidence: 99%

Modes of Action of 1,8-Cineol in Infections and Inflammation

et al. 2023

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The monoterpene 1,8-Cineol is a natural plant-based therapeutic agent that is commonly applied to treat different inflammatory diseases due to its mucolytic, anti-microbial and anti-inflammatory properties. It has become increasingly clear in the recent years that 1,8-Cineol spreads almost everywhere in the human body after its oral administration, from the gut to the blood to the brain. Its anti-microbial potential and even its anti-viral effects have been observed to include numerous bacteria and fungi species. Many recent studies help to better understand the cellular and molecular immunological consequences of 1,8-Cineol treatment in inflammatory diseases and further provide information concerning the mechanistic modes of action in the regulation of distinct inflammatory biosynthetic pathways. This review aims to present a holistic and understandable overview of the different aspects of 1,8-Cineol in infections and inflammation.

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Section: Anti-microbial and Anti-viral Potentialmentioning

confidence: 99%

Modes of Action of 1,8-Cineol in Infections and Inflammation

et al. 2023

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“…In this context, essential oils from Eucalyptus globulus with 1,8-Cineol as the main metabolite (65.83%) have been shown to inhibit Streptococcus mutans even in biofilm cultures, emulating dental plaque conditions 34 . It is most likely that biofilm formation is a major problem concerning the efficacy of anti-microbial treatment due to limited penetrability.…”

Section: Discussionmentioning

confidence: 99%

Anti-inflammatory response to 1,8-Cineol and associated microbial communities in Otitis media patients

Leichtle,

Lupatsii,

Graspeuntner

et al. 2024

Sci Rep

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Chronic Otitis Media (COM) is defined as long term inflammation and colonization with pathogenic bacteria due to a defect or retraction of the tympanic membrane. Surgical interventions are often augmented by antibiotic resistance development and therefore, off-label treatment using the natural drug 1,8-Cineol was carried out. All COM patients underwent antibiotic therapy and middle ear surgery and developed antibiotic resistances. Microbiological investigations from the auditory canal and stool samples were performed in correlation with the clinical course. Therapy of COM patients with 1,8-Cineol revealed a clear reduction of inflammatory microbes P. aeruginosa and Proteus mirabilis in ear samples as well as intestinal Prevotella copri, which was associated with an improved clinical outcome in certain individuals. The present off-label study revealed manifold anti-inflammatory effects of the natural monoterpene 1,8-Cineol in Otitis media patients. A better understanding of the underlying mechanisms will improve the current treatment options and possible forms of application of this natural drug.

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“…Generally, EOs have high contents (20-70%) of two or three major phytoconstituents; other compounds are quantified in trace concentrations [3]. The pharmacological potential of EOs has been extensively explored: ranging from antioxidant [4,5], anti-inflammatory [6,7], immunomodulatory [8,9], antinociceptive [10,11], antiulcer [12,13], anticancer [14,15], insecticidal [16,17], larvicidal [18,19], anthelmintic [20,21], antiviral [22,23], antibacterial and antibiofilm [24][25][26][27][28] properties.…”

Section: Introductionmentioning

confidence: 99%

Antibacterial and Antibiofilm Effects of Different Samples of Five Commercially Available Essential Oils

Neagu

Popovici

Ionescu³

et al. 2023

Antibiotics

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Essential oils (EOs) have gained economic importance due to their biological activities, and increasing amounts are demanded everywhere. However, substantial differences between the same essential oil samples from different suppliers are reported—concerning their chemical composition and bioactivities—due to numerous companies involved in EOs production and the continuous development of online sales. The present study investigates the antibacterial and antibiofilm activities of two to four samples of five commercially available essential oils (Oregano, Eucalyptus, Rosemary, Clove, and Peppermint oils) produced by autochthonous companies. The manufacturers provided all EOs’ chemical compositions determined through GC-MS. The EOs’ bioactivities were investigated in vitro against Gram-positive (Staphylococcus aureus) and Gram-negative bacteria (Escherichia coli and Pseudomonas aeruginosa). The antibacterial and antibiofilm effects (ABE% and, respectively, ABfE%) were evaluated spectrophotometrically at 562 and 570 nm using microplate cultivation techniques. The essential oils’ calculated parameters were compared with those of three standard broad-spectrum antibiotics: Amoxicillin/Clavulanic acid, Gentamycin, and Streptomycin. The results showed that at the first dilution (D1 = 25 mg/mL), all EOs exhibited antibacterial and antibiofilm activity against all Gram-positive and Gram-negative bacteria tested, and MIC value > 25 mg/mL. Generally, both effects progressively decreased from D1 to D3. Only EOs with a considerable content of highly active metabolites revealed insignificant differences. E. coli showed the lowest susceptibility to all commercially available essential oils—15 EO samples had undetected antibacterial and antibiofilm effects at D2 and D3. Peppermint and Clove oils recorded the most significant differences regarding chemical composition and antibacterial/antibiofilm activities. All registered differences could be due to different places for harvesting the raw plant material, various technological processes through which these essential oils were obtained, the preservation conditions, and complex interactions between constituents.

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Inhibitory Activity of Essential Oils of Mentha spicata and Eucalyptus globulus on Biofilms of Streptococcus mutans in an In Vitro Model

Cited by 7 publications

References 41 publications

Modes of Action of 1,8-Cineol in Infections and Inflammation

Modes of Action of 1,8-Cineol in Infections and Inflammation

Anti-inflammatory response to 1,8-Cineol and associated microbial communities in Otitis media patients

Antibacterial and Antibiofilm Effects of Different Samples of Five Commercially Available Essential Oils

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