The aim of this study was to evaluate the inhibitory activity of the commercially available essential oils of Mentha spicata (spearmint) and Eucalyptus globulus (eucalyptus) on Streptococcus mutans ATCC 25175 biofilms in vitro, emulating dental plaque conditions. The composition of the essential oils (EOs) was determined using gas chromatography coupled with mass spectrometry (GC-MS), with the main metabolites being Carvone (57.93%) and Limonene (12.91%) for Mentha spicata and 1,8-Cineole (Eucalyptol) (65.83%) for Eucalyptus globulus. The inhibitory activity was evaluated using the methods of agar-well diffusion and colorimetric microdilution. The inhibition halos were 18.3 ± 0.47 mm and 27.0 ± 0.82 mm, and the MICs were 1.8484 mg/mL and 1.9168 mg/mL for the EOs of Mentha spicata and Eucalyptus globulus, respectively. The activity against the biofilms was evaluated on a substrate of bovine enamel pieces using a basal mucin medium (BMM) in anaerobic conditions with daily sucrose exposition cycles in order to emulate oral cavity conditions. The EOs were applied in a concentration of 0.5% in a sterile saline vehicle with 1% polysorbate 20. After 72 h of cultivation, a significant reduction was observed (p < 0.001%) on the biofilm biomass, which was evaluated by its turbidity in suspension and using a count of the recoverable organisms with regards to the control. The effects of the Eos were not significantly distinct from each other. The EOs showed antimicrobial activity against both the Streptococcus mutans planktonic and biofilm cultures. Thus, EOs may have great potential for the development of pharmaceutical and sanitary products for oral health.
Artículo Investigación RESUMEN: El eugenol es un alilbenceno con actividad antifúngica, diversos estudios han demostrado su capacidad de inhibir el crecimiento de Candida albicans. Se ha propuesto la formación de aductos entre un derivado del eugenol (2-metoxi-4-alilideno-2,5-hexadieno-1-ona) y glutatión como un mecanismo de estrés oxidativo. Asimismo, otro derivado del eugenol, epóxido de eugenol, tiene teóricamente la capacidad para formar aductos. Por lo tanto, este estudio determinó in silico las propiedades fisicoquímicas de estos aductos y su reactividad en microorganismos aeróbicos. Se encontró que estos aductos tienen baja hidrofobicidad, por lo que, tendrían baja capacidad para atravesar membranas biológicas y por tanto una distribución intracelular. Asimismo, las reacciones químicas que originan estos aductos son termodinámicamente favorables. Por último, estos aductos son susceptibles a modificaciones químicas en microorganismos aeróbicos; estas reacciones podrían ser catalizadas por enzimas como el citocromo P450.PALABRAS CLAVE: Aductos con glutatión; Candida albicans; derivados del eugenol.ABSTRACT: Eugenol is an allylbenzene with antifungal activity, many studies have demonstrated its ability to inhibit the growth of Candida albicans. The formation of adducts between a derivative of eugenol (2-methoxy-4-allylidene-2,5-ciclohexadiene-1-one) and glutathione as a mechanism of oxidative stress has been proposed. Likewise, another eugenol derivative, eugenol epoxide, theoretically has the capacity to form adducts. Therefore, this study determined in silico the physicochemical properties of these adducts and their reactivity in aerobic microorganisms. It was found a Rabanal, J. & Ruiz-Quiroz, J. (2018). Estudio in silico de la reactividad y propiedades fisicoquímicas de aductos de epóxido de eugenol y 2-metoxi-4-alilideno-2,5-ciclohexadieno-1-ona con glutatión en Candida albicans. Rev.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.