Inhibitory activity of antibiotic‐producing marine bacteria against fish pathogens

Abstract

The activity of antibiotic-producing marine bacteria was assayed against bacterial fish pathogens belonging to the genera Vibrio, Aeromonas, Pasteurella, Edwardsiella, Yersinia and Pseudomonas with the aim of evaluating the possible use of these marine strains for controlling epizootics in aquaculture. Inhibition tests on solid medium showed that, in general, the majority of fish bacteria were strongly sensitive to the marine bacteria. Only two strains (Edwardsiella tarda and Pseudomonas aeruginosa), were resistant to all the antibiotic-producing strains. The results of antagonism assays in sea water, however, varied according to the fish pathogens examined. Experiments conducted using cell-free supernatant fluids of marine bacteria demonstrated the involvement of antibiotic substances in the inhibition of fish pathogens.

Antagonistic activity of cellular components of Pseudomonas species against Aeromonas hydrophila

“…Other workers have also attempted to use other pathogens, e.g. Edwardsiella tarda, Enterococcus seriolicida, Pasteurella piscicida, Yersinia ruckeri (Dopazo et al, 1988;Austin et al, 1995;Ruiz et al, 1996;Sugita et al, 1996;Byun et al, 1997;Gibson et al, 1998). Some bacteria are antagonistic to viruses (Kamei et al, 1987(Kamei et al, , 1988Direkbusarakom et al, 1998) and they may be efficient for biocontrol of viral diseases (Maeda et al, 1997).…”

“…It has been recorded that antimicrobial substances produced by some bacteria seem to play an important role in antagonising other bacteria in aquatic ecosystems (Dopazo et al, 1988;Sugahara et al, 1988). It has also been reported that intestinal bacteria from turbot show an inhibitory effect on fish pathogenic bacteria (Westerdahl et al, 1991;Olsson et al, 1992;Sugita et al, 1997).…”

Screening of Autochthonous Intestinal Microbiota as Candidate Probiotics Isolated from Four Freshwater Teleosts

“…The antagonistic assay was done following the double agar layer method described elsewhere 24 . Briefly, macro colonies of the test candidates were spread on Mueller Hinton agar (pH 7.0) plates, grown for 48 h in an incubator (30  1C) and killed by chloroform vapour.…”

Vibro-inhibiting Marine Bacteria Isolated from Black Tiger Shrimp Hatchery.

“…All of the four species have been isolated from the Mediterranean Sea. Bacteria of this genus were also isolated from intertidal green and brown algae in the north-west of Spain and displayed inhibitory activity against bacterial fish pathogens belonging to the genera Vibrio, Aeromonas, Pasteurella, Edwardsiella, and Yersinia (Dopazo et al, 1988).…”

“…Interaction between each strain of antibiotic-produc ing N01, N06, N18, N29 and each species of V. alginolyticus V07, V. parahaemolyticus V13 and V. harveyi V22 is given in (Gauthier and Flatau, 1976;Gauthier, 1977;Imada et al, 1985;Dopazo et al, 1988). (Krieg and Holt, 1984), the genus Alteromonas was characterized into 11 species and 4 of these, A. rubra, A. luteoviolacea, A.…”