Antagonistic activity of cellular components of Pseudomonas species against Aeromonas hydrophila

Abstract

Antagonistic effects of Pseudomonas fluorescens, P. aeruginosa and P. putida were studied against 12 strains of Aeromonas hydrophila (Ah1-Ah12). Four different fractions of cellular component (i.e. whole cell product, heat killed whole cell product, intra cellular product and extra cellular product) of all Pseudomonas species were equally effective in reducing growth of A. hydrophila strains, as measured by the zone of inhibition in an in vitro sensitivity test and have potential action against A. hydrophila infection in fishes. D

Protective Effect of Pseudomonas Fluorescens as a Probiotic in Controlling Fish Pathogens

“…And, Serum samples centrifugation at 3000 RPM of the total protein content method described by [27] colorimetric method at wave [28]. [16][17][18]32]. Pseudomonas effect in vitro may be attributed several antibiotic-like substances as phenazine antibiotic [33], non-nitrogenand siderophores production for iron [20,35,36].…”

“…In particular, innate immunity has been shown to be affected by feeding fish with probiotic [15]. Non-pathogenic pseudomonas can be used for control of some bacterial pathogens [2] in vivo and in vitro antibacterial activity against Aeromonas hydrophila [16,17,18], Aeromonas salmonicida [19], Staphylococcus aureus and Aeromonas sobria [20], Saprolegnia and other fungi [21,22], Vibrio [23] and Flavobacterium psychrophilum [24].…”

Antagonistic activity of extra cellular product and component bacteria of Pseudomonas sp. against Aeromonas hydrophila from tilapia aquaculture in East Borneo

“…bacteria possessed antibacterial activity against A. hydrophila pathogen at 24 h and decreased after 48 h. All cellular components from the bacteria displayed activity to suppress the pathogen bacteria with inhibition zone less than or 10 mm except for crude ECP at 11 mm, which was categorized as resistant or has weak antibacterial activity. However, Das et al's (2006) [10] investigation showed that heat whole cells and killed whole cell product from some strains of Pseudomonas sp. could suppress the growth of several strains of A. hydrophila.…”

“…Research about controlling pathogenic bacteria in fish aquaculture is already being developed in aquaculture [6,10,11]. Several types of Pseudomonas sp.…”

Antibiotics in Aquaculture – Use, Abuse and Alternatives

“…The most studied are lactic acid bacteria (LAB) (Lactobacillus, Carnobacterium, Enterococcus, Lactococcus, Micrococcus, Streptococcus, and Weissella) (Balcazar et al, 2008;Balcázar et al, 2007;Hagi & Hoshino, 2009;Pérez-Sánchez et al, 2011;Vazquez et al, 2005;Villamil et al, 2002), Bacillus (Ai et al, 2011;Antony et al, 2011;Balcázar & Rojas-Luna, 2007;Bandyopadhyay & Das Mohapatra, 2009;Ochoa-Solano & Olmos-Soto, 2006;Liu et al, 2009;Nakayama et al, 2009;Newaj-Fyzul et al, 2007;Olmos et al, 2011;Salinas et al, 2005;Sun et al, 2010;Vaseeharan & Ramasamy, 2003), Vibrio (Fjellheim et al, 2007;Thompson et al, 2010), Pseudomonas (Abd El-Rhman et al, 2009;Chythanya, 2002;Das et al, 2006;Preetha et al, 2007;Ström-Bestor, Wiklund, 2011), and Aeromonas (Irianto et al, 2003;Lategan et al, 2006;. Yeasts (Saccharomyces, Debaryomyces) (Abdeltawwab et al, 2008;Reyes-Becerril et al, 2008;Tovar-Ramírez et al, 2010), bacterial spore formers (Hong et al, 2005) and recently Actinobacteria have also generated interest due to their high metabolic potential (Das et al, 2010;You et al, 2007).…”