Inhibitors of polygalacturonase and laccase of Botrytis cinerea and their application to the control of this fungus

Sansone, G; Rezza, Irma; Fernandez, Gaston; Calvente, Viviana; Benuzzi, Delia; Sanz, María I.

doi:10.1016/j.ibiod.2010.09.010

Cited by 12 publications

(14 citation statements)

References 16 publications

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“…Most of the pathogens employ lignolytic and hydrolytic enzymes for their invasion to the host cell wall (Sansone et al . ). The lignolytic enzymes act as detoxifying enzymes to overcome plant defence response and it plays an important role in pathogenicity (Kudanga et al .…”

Section: Discussionmentioning

confidence: 97%

“…Most of the pathogens employ lignolytic and hydrolytic enzymes for their invasion to the host cell wall (Sansone et al 2011). The lignolytic enzymes act as detoxifying enzymes to overcome plant defence response and it plays an important role in pathogenicity (Kudanga et al 2008), the hydrolytic enzymes while invading play another role of nourishing the pathogen by degrading the major components of the cell wall in the host.…”

Section: Discussionmentioning

confidence: 99%

“…The study of the anatomy of oil palm tree shows us that the major constituents in its trunk is cellulose (41Á2%) followed by hemicelluloses (34Á4%) and lignin (17Á1%) (Sun and Tomkinson 2001;Murai et al 2009). To enter into the plant cells, the pathogen requires two types of enzymes for two types of processes; first, the oxidative enzymes to overcome the plant defence system, and second, the hydrolytic enzymes to gain energy (Sansone et al 2011). The hydrolytic enzymes degrade the major (cellulose and hemicelluloses) constituents in the cell wall, thus it aids in the penetration as well nourishment of the pathogen by breaking down the cellulose components or by releasing the nutrients from the disturbed cell of the host.…”

Section: Introductionmentioning

confidence: 99%

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Inhibition and kinetic studies of cellulose- and hemicellulose-degrading enzymes ofGanoderma boninenseby naturally occurring phenolic compounds

et al. 2018

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Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Inhibition and kinetic studies of cellulose- and hemicellulose-degrading enzymes ofGanoderma boninenseby naturally occurring phenolic compounds

et al. 2018

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“…The selected strains, BNM 0527 and BNM 0528, have different pathogenicity characteristics. Despite the fact that the growth rate of B. cinerea BNM 0528 is greater than that of strain BNM 0527 (Sansone et al …”

Section: Resultsmentioning

confidence: 99%

“…According to Sansone et al . (), the highest pathogenicity of B. cinerea BNM 0527 is related to the higher production of polygalacturonase and laccase, which are attack enzymes of this pathogen.…”

Section: Resultsmentioning

confidence: 99%

Evaluation of Rhodosporidium fluviale as biocontrol agent against Botrytis cinerea on apple fruit

Sansone

Lambrese

Calvente

et al. 2018

Lett Appl Microbiol

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Significance and Impact of the Study: Botrytis cinerea Pers: Fr, which causes gray mold of fruits and vegetables around the world, is difficult to control successfully because it is genetically variable and rapidly develops resistance to the chemicals commonly used for its control. This study is a contribution to the biocontrol of this phytopathogen fungus. The evaluation of the native yeast Rhodosporidium fluviale as biocontrol agent and the elucidation of possible mechanisms of action, including the participation of nonviable cells of this yeast, have not been reported up to date.

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Development of simple, scalable protease production from Botrytis cinerea

Self

Harrison

Te’o

et al. 2022

Appl Microbiol Biotechnol

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Heat haze-forming proteins are stable during winemaking and are typically removed via adsorption to bentonite. Proteolytic degradation is an alternative method to prevent wine-haze and offers the opportunity to reduce the environmental impacts and labor cost of the process. Herein, we describe the development of a production system for Botrytis cinerea proteases for the enzymatic degradation of heat haze-forming proteins. The effect of culture medium on the secretion of glucan by B. cinerea was investigated and methods to inactivate B. cinerea laccase in liquid culture medium were assessed. Protease production by B. cinerea was scaled up from 50 mL in shake flasks to 1 L in bioreactors, resulting in an increase in protease yield from 0.30 to 3.04 g L−1. Glucan secretion by B. cinerea was minimal in culture medium containing lactose as a carbon source and either lactic or sulfuric acid for pH control. B. cinerea laccases were inactivated by reducing the pH of culture supernatant to 1.5 for 1 h. B. cinerea proteases were concentrated and partially purified using ammonium sulfate precipitation. SWATH-MS identified aspartic acid protease BcAP8 amongst the precipitated proteins. These results demonstrate a simple, affordable, and scalable process to produce proteases from B. cinerea as a replacement for bentonite in winemaking. Key points • Isolates of B. cinerea that produce proteases with potential for reducing wine heat-haze forming proteins were identified. • Media and fermentation optimization increased protease yield tenfold and reduced glucan secretion. • Low pH treatment inactivated laccases but not proteases. Graphical abstract

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Inhibitors of polygalacturonase and laccase of Botrytis cinerea and their application to the control of this fungus

Cited by 12 publications

References 16 publications

Inhibition and kinetic studies of cellulose- and hemicellulose-degrading enzymes ofGanoderma boninenseby naturally occurring phenolic compounds

Inhibition and kinetic studies of cellulose- and hemicellulose-degrading enzymes ofGanoderma boninenseby naturally occurring phenolic compounds

Evaluation of Rhodosporidium fluviale as biocontrol agent against Botrytis cinerea on apple fruit

Development of simple, scalable protease production from Botrytis cinerea

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