The present work aimed to characterize and to improve the production of the siderophore produced by Kosakonia radicincitans and apply it to control of phytopathogenic fungi. The siderophore, characterized by liquid chromatography coupled to tandem mass detection (LC-MS/MS), was identified as enterochelin. The improvement of its production was carried out using different conditions. The higher production of enterochelin (45 mg g -1 dry weight biomass) was reached in a medium with lactose as the carbon source, 0.82 mol.L -1 of iron, at 28°C and 200 rpm.The siderophore at 60 mg L -1 of concentration inhibited the conidia germination and the mycelial growth of Penicillium expansum, Botrytis cinerea, Rhizopus sp., Alternaria sp. and Cladosporium cladosporoides. Biocontrol of Botrytis cinerea and Penicillium expansum were performed in apple fruit using Kosakonia radicincitans and enterochelin, alone or in a mixture. The mixture was the more effective. In this case, the reduction of decay provoked for B.cinerea reached 52%.
Significance and Impact of the Study: Botrytis cinerea Pers: Fr, which causes gray mold of fruits and vegetables around the world, is difficult to control successfully because it is genetically variable and rapidly develops resistance to the chemicals commonly used for its control. This study is a contribution to the biocontrol of this phytopathogen fungus. The evaluation of the native yeast Rhodosporidium fluviale as biocontrol agent and the elucidation of possible mechanisms of action, including the participation of nonviable cells of this yeast, have not been reported up to date.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.