Production of siderophores by the bacterium Kosakonia radicincitans and its application to control of phytopathogenic fungi

Abstract

The present work aimed to characterize and to improve the production of the siderophore produced by Kosakonia radicincitans and apply it to control of phytopathogenic fungi. The siderophore, characterized by liquid chromatography coupled to tandem mass detection (LC-MS/MS), was identified as enterochelin. The improvement of its production was carried out using different conditions. The higher production of enterochelin (45 mg g -1 dry weight biomass) was reached in a medium with lactose as the carbon source, 0.82 mol.L -1 of iron, at 28°C and 200 rpm.The siderophore at 60 mg L -1 of concentration inhibited the conidia germination and the mycelial growth of Penicillium expansum, Botrytis cinerea, Rhizopus sp., Alternaria sp. and Cladosporium cladosporoides. Biocontrol of Botrytis cinerea and Penicillium expansum were performed in apple fruit using Kosakonia radicincitans and enterochelin, alone or in a mixture. The mixture was the more effective. In this case, the reduction of decay provoked for B.cinerea reached 52%.

Development and Application of Low-Cost and Eco-Sustainable Bio-Stimulant Containing a New Plant Growth-Promoting Strain Kosakonia pseudosacchari TL13

“…Siderophore producing bacteria can improve plant growth by reducing the Fe availability for the phytopathogens and increasing nutrient availability to the plant ( Ahmed and Holmström, 2014 ). As expected, the two K. pseudosacchari strains TL8 and TL13 were able to produce iron chelating siderophores, a trait commonly present in Kosakonia genus as largely reported by the literature ( Arora and Verma, 2017 ; Chimwamurombe et al, 2016 ; Lambrese et al, 2018 ). Siderophore production could be involved also in disease suppression.…”

Freeze-drying of a mixture of bacterium and yeast for application in postharvest control of pathogenic fungi

“…As was reported by Calvo et al [3], Penicillium expansum and Botrytis cinerea were controlled by the mixture. The capacity of C. laurentii and K. radicincitans to control postharvest disease was demonstrated in previous works by Navarta et al [16] and Lambrese et al [12]. By using mixtures, it was shown that the combination of two microorganisms with different nutritional requirements, different ecological niches, and different antagonistic abilities improved the control of different phytopathogenic molds [3,4,7,18,24].…”

“…The apples were incubated at 4 °C and 90 ± 5% RH. Samples were taken at 0 (prior to storage), 3,6,9,12,15,20,25,30, and 40 days. The wounded tissue was removed using a cork borer (6 mm internal diameter), suspended in 50 mL of sterile distilled water, and shaken on a rotatory shaker for 20 min at 240 rpm.…”

“…We also developed lyophilized formulations with Cryptococcus laurentii [16]. Lately, we have isolated and tested as BCA, the bacterium Kosakonia radicincitans [12], which in combination with C. laurentii, was effective in controlling P. expansum in apple (unpublished results). To date, we have not found reports about formulations containing K. radicincitans or mixtures of this bacterium with other BCAs.…”

A Streptomyces morookaensis strain promotes plant growth and suppresses Fusarium wilt of banana

“…Siderophores have been widely reported to be involved in antagonism through competition for iron with phytopathogenic agents during their progression within host tissues (Gokarn and Pal 2018;Lambrese et al 2018). The CAS assay showed that S. morookaensis strain Sm4-1986 did produce siderophores, and characteristic analysis showed that the siderophore produced by this strain belongs to the type of carboxylates.…”