Inhibition and kinetic studies of cellulose- and hemicellulose-degrading enzymes of<i>Ganoderma boninense</i>by naturally occurring phenolic compounds

Surendran, Arthy; Siddiqui, Yasmeen; Ali, Nur Shidaa Mohd; Manickam, Sivakumar

doi:10.1111/jam.13717

Cited by 23 publications

(17 citation statements)

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“…It can be postulated that pectin-acting PG work synergistically with hemicellulases for Ganoderma infection in similar fashion to necrotrophic infection and virulence of many phytopathogens including Fusarium spp., the main causative agents of vascular wilt and head blight diseases in important crops (Gómez-Gómez et al, 2002; Chen et al, 2012b; Paccanaro et al, 2017). Hemi-cellulosic digestion activities of G. boninense were previously demonstrated to assist the fungal growth on oil palm hence supporting the association of these PHI genes as potential pathogenicity factors in oil palm infection (Surendran et al, 2017; Surendran et al, 2018).…”

Section: Resultsmentioning

confidence: 77%

“…Lignocellulolytic enzymes of G. boninense have been shown to be predominant in instigating oil palm infection and cell wall-degrading processes (Goh, Ganeson & Supramaniam, 2014; Jumali & Ismail, 2017; Surendran et al, 2018). Direct roles of CWDEs in the hemibiotrophic infection of oil palm roots were first demonstrated via macroscopic examination of enzymatically-degraded root outer cell layers and invaded root and stem tissues of G. boninense -infected tissues (Rees, 2006; Rees et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

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Insight into plant cell wall degradation and pathogenesis of Ganoderma boninense via comparative genome analysis

Ramzi

Ruslan

et al. 2019

PeerJ

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BackgroundG. boninense is a hemibiotrophic fungus that infects oil palms (Elaeis guineensis Jacq.) causing basal stem rot (BSR) disease and consequent massive economic losses to the oil palm industry. The pathogenicity of this white-rot fungus has been associated with cell wall degrading enzymes (CWDEs) released during saprophytic and necrotrophic stage of infection of the oil palm host. However, there is a lack of information available on the essentiality of CWDEs in wood-decaying process and pathogenesis of this oil palm pathogen especially at molecular and genome levels.MethodsIn this study, comparative genome analysis was carried out using the G. boninense NJ3 genome to identify and characterize carbohydrate-active enzyme (CAZymes) including CWDE in the fungal genome. Augustus pipeline was employed for gene identification in G. boninense NJ3 and the produced protein sequences were analyzed via dbCAN pipeline and PhiBase 4.5 database annotation for CAZymes and plant-host interaction (PHI) gene analysis, respectively. Comparison of CAZymes from G. boninense NJ3 was made against G. lucidum, a well-studied model Ganoderma sp. and five selected pathogenic fungi for CAZymes characterization. Functional annotation of PHI genes was carried out using Web Gene Ontology Annotation Plot (WEGO) and was used for selecting candidate PHI genes related to cell wall degradation of G. boninense NJ3.ResultsG. boninense was enriched with CAZymes and CWDEs in a similar fashion to G. lucidum that corroborate with the lignocellulolytic abilities of both closely-related fungal strains. The role of polysaccharide and cell wall degrading enzymes in the hemibiotrophic mode of infection of G. boninense was investigated by analyzing the fungal CAZymes with necrotrophic Armillaria solidipes, A. mellea, biotrophic Ustilago maydis, Melampsora larici-populina and hemibiotrophic Moniliophthora perniciosa. Profiles of the selected pathogenic fungi demonstrated that necrotizing pathogens including G. boninense NJ3 exhibited an extensive set of CAZymes as compared to the more CAZymes-limited biotrophic pathogens. Following PHI analysis, several candidate genes including polygalacturonase, endo β-1,3-xylanase, β-glucanase and laccase were identified as potential CWDEs that contribute to the plant host interaction and pathogenesis.DiscussionThis study employed bioinformatics tools for providing a greater understanding of the biological mechanisms underlying the production of CAZymes in G. boninense NJ3. Identification and profiling of the fungal polysaccharide- and lignocellulosic-degrading enzymes would further facilitate in elucidating the infection mechanisms through the production of CWDEs by G. boninense. Identification of CAZymes and CWDE-related PHI genes in G. boninense would serve as the basis for functional studies of genes associated with the fungal virulence and pathogenicity using systems biology and genetic engineering approaches.

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Section: Resultsmentioning

confidence: 77%

Section: Introductionmentioning

confidence: 99%

Insight into plant cell wall degradation and pathogenesis of Ganoderma boninense via comparative genome analysis

Ramzi

Ruslan

et al. 2019

PeerJ

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“…However, fungal cultures usually exhibit long incubation times and greater or lesser carbohydrates loss as a function of fungal strain, while enzymatic pretreatments require costly processes of enzyme production, purification, and the preparation of enzymatic cocktails. In addition, enzyme inhibition by some natural-occurring phenolics [45], the stability of ligninolytic enzymes and consumption of H 2 O 2 as a co-substrate for catalytic cycle of peroxidases, are limiting factors. Thus, WRF are an attractive option for biomass delignification and the deconstruction of the cell wall matrix, since this is an environment-friendly biotechnology and also a low-cost process.…”

Section: Pretreatment: Lignocellulose Composition Enzymatic Activitimentioning

confidence: 99%

Pretreatment of Grape Stalks by Fungi: Effect on Bioactive Compounds, Fiber Composition, Saccharification Kinetics and Monosaccharides Ratio

Fernandes

Fraga

Sousa

et al. 2020

IJERPH

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Grape stalks, an inedible lignocellulosic residue from winemaking and agro-industrial grape juice production, can be valorized as a source of bioactive compounds and as feedstock for the saccharification and bioconversion of soluble sugars. Solid-state fermentation (SSF) by six white-rot fungi was applied as pretreatment. Fiber composition, free radical scavenging activity, four ligninolytic, and three hydrolytic enzyme activities were determined. Saccharification kinetics, yield, and productivity were evaluated and complemented with scanning electron microscopy (SEM), high performance liquid chromatography (HPLC) quantification of monosaccharides, and principal component analysis (PCA). After SSF, the biomass exhibited a drastic free radical scavenging activity decrease and the main enzymes produced were manganese-dependent peroxidase and xylanase. Scanning electron microscopy revealed the erosion of cell walls, and PCA exhibited a negative correlation between saccharification, and neutral detergent fiber and acid detergent lignin. Phlebia rufa pretreated biomass gave the highest sugars yield and productivity, representing a nearly three-fold increase compared to untreated samples. Also, monosaccharides quantification revealed that the 1:1 ratio of glucose to the sum of xylose plus galactose changes to the value of 2:1 after pretreatment. In this work, and for the first time, P. rufa proved to be an effective pretreatment of grape stalks for the saccharification and further bioconversion into value-added chemicals. In addition, lignocellulolytic enzymes were also produced through SSF.

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“…The K m and V max values of different fungus varied for the same substrate (Surendran et al . ). Most of the laccase from fungus showed high affinity towards ABTS but the K m value of G. boninense laccase is only 66·6 μ mol l −1 .…”

Section: Discussionmentioning

confidence: 97%

Inhibition and kinetic studies of lignin degrading enzymes ofGanoderma boninenseby naturally occurring phenolic compounds

et al. 2018

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Inhibition and kinetic studies of cellulose- and hemicellulose-degrading enzymes ofGanoderma boninenseby naturally occurring phenolic compounds

Abstract: The study has proved a replacement for chemical controls of G. boninense with naturally occurring phenolic compounds.

Cited by 23 publications

References 45 publications

Insight into plant cell wall degradation and pathogenesis of Ganoderma boninense via comparative genome analysis

Insight into plant cell wall degradation and pathogenesis of Ganoderma boninense via comparative genome analysis

Pretreatment of Grape Stalks by Fungi: Effect on Bioactive Compounds, Fiber Composition, Saccharification Kinetics and Monosaccharides Ratio

Inhibition and kinetic studies of lignin degrading enzymes ofGanoderma boninenseby naturally occurring phenolic compounds

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