Inhibitors of nitrogen fixation in extracts from Clostridium pasteurianum

Lockshin, Arnold; Burris, R. H.

doi:10.1016/0304-4165(65)90466-6

Cited by 70 publications

(38 citation statements)

References 18 publications

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“…C. pasteurianum nitrogenase has a higher Ki for inhibition of N2 reduction by H2 (30) and shows different specificities for nucleotides and nucleotide analogs (54). C. pasteurianum nitrogenase components fail to complement components from other eubacterial Mo-nitrogenases and can form inactive complexes with them (13).…”

Section: L D Y T P C E E V T R E a V T I Nmentioning

confidence: 99%

Cloning, DNA sequencing, and characterization of a nifD-homologous gene from the archaeon Methanosarcina barkeri 227 which resembles nifD1 from the eubacterium Clostridium pasteurianum

Chien

Zinder

1994

J Bacteriol

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Section: L D Y T P C E E V T R E a V T I Nmentioning

confidence: 99%

Cloning, DNA sequencing, and characterization of a nifD-homologous gene from the archaeon Methanosarcina barkeri 227 which resembles nifD1 from the eubacterium Clostridium pasteurianum

Chien

Zinder

1994

J Bacteriol

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“…N 2 reduction by Mo-and V-nitrogenases is strongly inhibited by carbon monoxide (CO) (5)(6)(7)(8)(9)(10). It is assumed that CO blocks electron flow in Mo-and V-nitrogenases at different stages, because hydrogen production by Mo-nitrogenase is largely unaffected by CO, whereas V-nitrogenase-catalyzed H 2 evolution decreases gradually with increasing CO concentrations (6).…”

mentioning

confidence: 99%

NifA- and CooA-Coordinated cowN Expression Sustains Nitrogen Fixation by Rhodobacter capsulatus in the Presence of Carbon Monoxide

et al. 2014

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Rhodobacter capsulatus fixes atmospheric dinitrogen via two nitrogenases, Mo-and Fe-nitrogenase, which operate under different conditions. Here, we describe the functions in nitrogen fixation and regulation of the rcc00574 (cooA) and rcc00575 (cowN) genes, which are located upstream of the structural genes of Mo-nitrogenase, nifHDK. Disruption of cooA or cowN specifically impaired Mo-nitrogenase-dependent growth at carbon monoxide (CO) concentrations still tolerated by the wild type. The cooA gene was shown to belong to the Mo-nitrogenase regulon, which is exclusively expressed when ammonium is limiting. Its expression was activated by NifA1 and NifA2, the transcriptional activators of nifHDK. AnfA, the transcriptional activator of Fe-nitrogenase genes, repressed cooA, thereby counteracting NifA activation. CooA activated cowN expression in response to increasing CO concentrations. Base substitutions in the presumed CooA binding site located upstream of the cowN transcription start site abolished cowN expression, indicating that cowN regulation by CooA is direct. In conclusion, a transcription factor-based network controls cowN expression to protect Mo-nitrogenase (but not Fe-nitrogenase) under appropriate conditions.

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“…Nitrogen fixation during growth of Azotobacter spp. was known to be inhibited by the azide ion (Wilson & Roberts, 1954) or nitrous oxide (Rakestraw & Roberts, 1957), and inhibition of cell-free nitrogen fixation by the azide or cyanide ion, or by nitrous oxide, was demonstrated by Lockshin & Burris (1965). Scholhorn & Burris (1966, 1967a reported that azide was reduced by nitrogenase, and Hardy & Knight (1966b reported that cyanide or nitrous oxide was also reduced by nitrogenase preparations.…”

mentioning

confidence: 99%

The kinetics of the reduction of isocyanides, acetylenes and the cyanide ion by nitrogenase preparation from Azotobacter chroococcum and the effects of inhibitors

Kelly

1968

Biochemical Journal

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1. Nitrogen-fixing preparations from Azotobacter chroococcum reduced substrates with the following Km values: methyl isocyanide, 1·8×10−4m; ethyl isocyanide, 2·5×10−2m; cyanide ion, 1·4×10−3m; acetylene, 1·2×10−4m. 2. Nitrogen, carbon monoxide or hydrogen competitively inhibited isocyanide reduction with the following Ki values: hydrogen, 1·3×10−3m; carbon monoxide, 6·8×10−6m; nitrogen, 4·3×10−4m. 3. Living nitrogen-fixing bacteria, and isolated clover nodules, formed methane from methyl isocyanide. 4. These results are discussed in relation to other work and possible mechanisms of nitrogen fixation.

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Inhibitors of nitrogen fixation in extracts from Clostridium pasteurianum

Cited by 70 publications

References 18 publications

Cloning, DNA sequencing, and characterization of a nifD-homologous gene from the archaeon Methanosarcina barkeri 227 which resembles nifD1 from the eubacterium Clostridium pasteurianum

Cloning, DNA sequencing, and characterization of a nifD-homologous gene from the archaeon Methanosarcina barkeri 227 which resembles nifD1 from the eubacterium Clostridium pasteurianum

NifA- and CooA-Coordinated cowN Expression Sustains Nitrogen Fixation by Rhodobacter capsulatus in the Presence of Carbon Monoxide

The kinetics of the reduction of isocyanides, acetylenes and the cyanide ion by nitrogenase preparation from Azotobacter chroococcum and the effects of inhibitors

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