1968
DOI: 10.1042/bj1070001
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The kinetics of the reduction of isocyanides, acetylenes and the cyanide ion by nitrogenase preparation from Azotobacter chroococcum and the effects of inhibitors

Abstract: 1. Nitrogen-fixing preparations from Azotobacter chroococcum reduced substrates with the following Km values: methyl isocyanide, 1·8×10−4m; ethyl isocyanide, 2·5×10−2m; cyanide ion, 1·4×10−3m; acetylene, 1·2×10−4m. 2. Nitrogen, carbon monoxide or hydrogen competitively inhibited isocyanide reduction with the following Ki values: hydrogen, 1·3×10−3m; carbon monoxide, 6·8×10−6m; nitrogen, 4·3×10−4m. 3. Living nitrogen-fixing bacteria, and isolated clover nodules, formed methane from methyl isocyanide. 4. These r… Show more

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Cited by 47 publications
(15 citation statements)
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“…M.S.3 mass spectrometer either locally or by Dr C. W. Crane Acetylene and isocyanide reduction. All unequivocally nitrogen-king organisms SO far examined reduce acetylene to ethylene (Dilworth, 1966;Schollhorn & Burris, 1967) and methyl isocyanide to methane (Kelly, 1968). These reactions provide very sensitive tests for the presence of the nitrogenase system since the products can be detected by gas chromatography.…”
Section: Organismsmentioning
confidence: 99%
“…M.S.3 mass spectrometer either locally or by Dr C. W. Crane Acetylene and isocyanide reduction. All unequivocally nitrogen-king organisms SO far examined reduce acetylene to ethylene (Dilworth, 1966;Schollhorn & Burris, 1967) and methyl isocyanide to methane (Kelly, 1968). These reactions provide very sensitive tests for the presence of the nitrogenase system since the products can be detected by gas chromatography.…”
Section: Organismsmentioning
confidence: 99%
“…and for the strict anaerobe, Clostridium pasteurianum have been reported by several groups of workers (1)(2)(3)(4), few investigations have been made with the enzyme systems from the facultative anaerobes, Klebsiella pneumoniae and Bacillus polymyxa. Mahl and Wilson (5) reported the first cell-free extract that fixed N2 from K.…”
mentioning
confidence: 99%
“…Ethylene, like ammonia, does not complex strongly with N2ase, being neither inhibitory to N2 fixation nor reduced by N2ase (26,53,54). The affinity of C2H2 for N2ase is similar to that of N2 for N2ase (26,50,53,54,57). The rate of reduction is four times that of N2 and the rate of electron consumption is about 1.3 times that of N2 (58).…”
Section: Figure 3 Classes Of Substrates Reduced By N2asementioning
confidence: 96%
“…It is not known whether HCN or CN" is the actual substrate, since both species are present at reaction pH. The affinity for cyanide based on equilibrium concentration of HCN is intermediate between that for N2 or C2H2 and N20 or N3", while the affinity based on CN" concentration is substantially greater (28,46,50). The rate of electron consumption is less than that seen in N2 reduction, which may indicate partial inhibition of N2ase by cyanide and/or the generation of undetermined products.…”
Section: Figure 3 Classes Of Substrates Reduced By N2asementioning
confidence: 96%
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