Cloning, DNA sequencing, and characterization of a nifD-homologous gene from the archaeon Methanosarcina barkeri 227 which resembles nifD1 from the eubacterium Clostridium pasteurianum

Chien, Yueh-tyng; Zinder, Stephen H.

doi:10.1128/jb.176.21.6590-6598.1994

Cited by 72 publications

(92 citation statements)

References 45 publications

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“…Additional open reading frames in the adjacent regions on either side of the cluster have been tentatively identified as a flavine-containing oxidoreductase (upstream of nifH) and a periplasmic molybdate binding protein plus a molybdenum transporter (downstream of nifN). The order of the M. mazei nitrogen fixation genes is the same as in other diazotrophic methanogenic archaea and differs from most bacterial nif gene clusters in that the two nifI genes are located between nifH and nifD (Sibold et al 1991, Chien and Zinder 1994, Kessler et al 1998, Arcondeguy et al 2001. Phylogenetic analysis of the deduced amino acid sequence of the M. mazei Gö1 nif genes showed that they are most closely related to M. barkeri nif2 genes, suggesting that M. mazei Gö1 has a molybdenum-containing nitrogenase (Table 1).…”

Section: Sequencing and Nucleotide Analysis Of The Nif Gene Cluster Fmentioning

confidence: 93%

“…As with bacterial nitrogen fixation, nif gene transcription in methanogenic archaea is regulated by the availability of nitrogen (Souillard and Sibold 1989, Chien and Zinder 1994, Kessler et al 1998. However, because of the major differences between archaeal and bacterial transcription, it is likely that the mechanisms of methanogenic nif gene regulation differ significantly from bacterial nif regulation.…”

Section: Introductionmentioning

confidence: 99%

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Functional organization of a single nif cluster in the mesophilic archaeon Methanosarcina mazei strain Gö1

Ehlers

Veit²,

Gottschalk³

et al. 2002

Archaea

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SummaryThe mesophilic methanogenic archaeon Methanosarcina mazei strain Gö1 is able to utilize molecular nitrogen (N 2 ) as its sole nitrogen source. We have identified and characterized a single nitrogen fixation (nif ) gene cluster in M. mazei Gö1 with an approximate length of 9 kbp. Sequence analysis revealed seven genes with sequence similarities to nifH, nifI 1 , nifI 2 , nifD, nifK, nifE and nif N, similar to other diazotrophic methanogens and certain bacteria such as Clostridium acetobutylicum, with the two glnB-like genes (nifI 1 and nifI 2 ) located between nifH and nifD. Phylogenetic analysis of deduced amino acid sequences for the nitrogenase structural genes of M. mazei Gö1 showed that they are most closely related to Methanosarcina barkeri nif 2 genes, and also closely resemble those for the corresponding nif products of the grampositive bacterium C. acetobutylicum. Northern blot analysis and reverse transcription PCR analysis demonstrated that the M. mazei nif genes constitute an operon transcribed only under nitrogen starvation as a single 8 kb transcript. Sequence analysis revealed a palindromic sequence at the transcriptional start site in front of the M. mazei nifH gene, which may have a function in transcriptional regulation of the nif operon.

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Section: Sequencing and Nucleotide Analysis Of The Nif Gene Cluster Fmentioning

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Functional organization of a single nif cluster in the mesophilic archaeon Methanosarcina mazei strain Gö1

Ehlers

Veit²,

Gottschalk³

et al. 2002

Archaea

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“…Specialized characteristics such as those producing insect-toxic protein crystals or dinitrogen fixation give the appearance of having moved from genome to genome horizontally (Chien and Zinder, 1994;Galibert et al, 2001). Other, less obvious, features, no doubt have moved also.…”

Section: Horizontal Gene Transfer and Its Effect On Evolution Of Bactmentioning

confidence: 99%

“…Other examples of features that appear across a wide range of genera considered distantly related by some measures, but which might be closely related by other criteria, include complex metabolic functions important to biotechnology exemplified by the nitrogenase complex genes or aromatic biodegradation genes (Chien and Zinder 1994;Hirsch et al, 1995;Harwood and Parales, 1996). How this may happen may be explained by recent developments in genomics, which is the study of an organism's genetic material.…”

Section: Use Of Taxonomy As a Basis For Further Inquiriesmentioning

confidence: 99%

Safety Assessment of Transgenic Organisms, Volume 4

2010

Harmonisation of Regulatory Oversight in Biotechnology

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io s a fe t y nv ir on me nt ag ri cu lt ur e bi os af et y g r ic u lt u r e e n vi r o n m e n t gr ic ul tu re bi os af et y en vi ro nm en t n vi r o n m e n t b io s a fe t y a g r ic u lt u r e e n vi r o io sa fe ty en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en v io s a fe t y a g r ic u lt u r e e n vi r o n m e n t b io s a fe t y a g r ic u lt u r e e n vi r o n m e n t nv ir on me nt ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm e g r ic u lt u r e e n vi r o n m e n t b io s a fe t y a g r ic u lt u r e e n vi r o n m e n t b io s a fe t y a g r ic u lt u r e e n vi r o n m e n t b io s a f gr ic ul tu re bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t a n vi r o n m e n t b io s a fe t y a g r ic u lt u r e e n vi r o n m e n t b io s a fe t y a g r ic u lt u r e e n vi r o n m e n t b io s a fe t y a g r ic u lt u io sa fe ty en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e b io s a fe t y a g r ic u lt u r e e n vi r o n m e n t b io s a fe t y a g r ic u lt u r e e n vi r o n m e n t b io s a fe t y a g r ic u lt u r e e n vi r o n m nv ir on me nt ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en g r ic u lt u r e e n vi r o n m e n t b io s a fe t y a g r ic u lt u r e e n vi r o n m e n t b io s a fe t y a g r ic u lt u r e e n vi r o n m e n t b io s a f gr ic ul tu re bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t a n vi r o n m e n t b io s a fe t y a g r ic u lt u r e e n vi r o n m e n t b io s a fe t y a g r ic u lt u r e e n vi r o n m e n t b io s a fe t y a g r ic u lt u io sa fe ty en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e b io s a fe t y a g r ic u lt u r e e n vi r o n m e n t b io s a fe t y a g r ic u lt u r e e n vi r o n m e n t b io s a fe t y a g r ic u lt u r e e n vi r o n m e nv ir on me nt ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y e g r ic u lt u r e e n vi r o n m e n t b io s a fe t y a g r ic u lt u r e e n vi r o n m e n t b io s a fe t y a g r ic u lt u r e e n vi r o n m e n t b io s a f ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et...

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“…In these analyses (26,38), there were four major branches. Three branches contained nifH genes that function in nitrogen fixation, while the fourth branch contained genes that evidently participate in a process unrelated to nitrogen fixation.…”

Section: Evolution Of Nitrogen Fixationmentioning

confidence: 99%

Nitrogen Fixation In Methanogens: The Archaeal Perspective

Leigh

2000

Current Issues in Molecular Biology

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The methanogenic Archaea bring a broadened perspective to the field of nitrogen fixation. Biochemical and genetic studies show that nitrogen fixation in Archaea is evolutionarily related to nitrogen fixation in Bacteria and operates by the same fundamental mechanism. At least six nif genes present in Bacteria (nif H, D, K, E, N and X) are also found in the diazotrophic methanogens. Most nitrogenases in methanogens are probably of the molybdenum type. However, differences exist in gene organization and regulation. All six known nif genes of methanogens, plus two homologues of the bacterial nitrogen sensorregulator glnB, occur in a single operon in Methanococcus maripaludis. nif gene transcription in methanogens is regulated by what appears to be a classical prokaryotic repression mechanism. At least one aspect of regulation, post-transcriptional ammonia switch-off, involves novel members of the glnB family. Phylogenetic analysis suggests that nitrogen fixation may have originated in a common ancestor of the Bacteria and the Archaea.

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Cloning, DNA sequencing, and characterization of a nifD-homologous gene from the archaeon Methanosarcina barkeri 227 which resembles nifD1 from the eubacterium Clostridium pasteurianum

Abstract: (Res. Microbiol. 142:5-12, 1991)

Cited by 72 publications

References 45 publications

Functional organization of a single nif cluster in the mesophilic archaeon Methanosarcina mazei strain Gö1

Functional organization of a single nif cluster in the mesophilic archaeon Methanosarcina mazei strain Gö1

Safety Assessment of Transgenic Organisms, Volume 4

Nitrogen Fixation In Methanogens: The Archaeal Perspective

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