Inhibition of Xanthine Oxidase by Flavonoids

Objective: Flavonoids from the crude seeds extract of Pongamia pinnata L., dried fruit powder of Morinda citrifolia L., bark of Mangifera indica L., and rhizome of Zingiber officinale Rosc. were screened for xanthine oxidase (XO) inhibition at different concentration. The inhibitory potential of quercetin and allopurinol were used for the determination of 50% inhibitory concentration (IC 50 ) and K i values.Methods: Isolation of flavonoids from the plant extracts was processed by column chromatography and tested for XO inhibitory activity in the range of 6-800 µg/ml. Results:The results demonstrated that optimized flavonoids extract of P. pinnata L. exhibited promising XO inhibition. P. pinnata L., M. indica L., and Z. officinale Rosc. had IC 50 in the concentration of 8.74 mM, 1.09 mM, 5.4 mM and Ki 0.35 mM, 1.73 mM, 2.7 mM, respectively. Conclusion:The study showed that plant species under investigation exhibited XO inhibition by optimized flavonoid extract. P. pinnata L. indicated promising XO inhibition compared to other plant extracts. Flavonoids can be used as a potent inhibitor of XO an alternative to allopurinol.

Section: Discussionmentioning

confidence: 99%

“…Plants sources are rich in pharmacologically active compounds such as phenolics, tannins, flavonoids, and lignin [1]. Such kind of active constituents distributed in different parts of the plants and posses properties of antimicrobial, antimutagenic, anticarcinogenic, antiinflammatory, vasodilatation, and enzyme inhibitory activity [2].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Xanthine Oxidase Inhibitory Activity by Flavonoids From Pongamia Pinnata Linn

Bambrana

Sheela

2017

“…The structure-activity relationships exhibited that flavones and flavonols with a-7-hydroxyl group such as chrysin, luteolin, kaempferol, quercetin, myricetin, and isorhamnetin inhibited XO activity at low concentration. The previous research presented that kaempferol showed XO inhibitory activity at IC 50 value 0.67 µM [23].…”

Section: Structure Elucidationmentioning

confidence: 98%

Constituent and Antihyperuricemic Activity of Stelechocarpus Burahol Leaves Subfractions

Sunarni

Fidrianny²,

Iwo³

et al. 2017

Objective: The goal of this research was to evaluate antihyperuricemic activity of Stelechocarpus burahol leaves subfractions and isolate its chemical constituent of active subfraction.Methods: Ethyl acetate fraction from S. burahol extract was subfractionated by vacuum liquid chromatography, and the active subfractions were further subfractionated by classic column chromatography using isocratic eluent, followed by isolated chemical constituent from active subfraction. Hyperuricemic rat model was induced by given potassium oxonate intraperitoneally. The inhibitory effect of subfractions on the xanthine oxidase (XO) activity was determined using ultraviolet-visible spectrophotometry method.Results: Subfractions E.3, E.4, and E.5 significantly (p<0.05) reduced the serum uric acid (UA) level 43%, 46%, and 33%, respectively. The E.3, E.4, and E.5 have showed very weak XO inhibitory activity. Subfraction E.3.2 and E.4.3 significantly (p<0.05) reduced the serum UA level 29% and 38%, respectively, however still very weak effect on XO activity. Chemical constituent which was isolated from subfraction E.4.3 was kaempferol-3-O-rhamnoside. Conclusion:The subfractions of ethyl acetate fraction had antihyperuricemic activity in vivo but less effect on XO activity in vitro. Isolated compound in active antihyperuricemic of subfraction E.4.3 was kaempferol-3-O-rhamnoside.

“…Tumor promoters such as lipoxygenases and arachidonate metabolizing enzymes, and cyclooxygenases which are phorbol esters activate or induce prooxidant and flavonoids are specifically effective at xanthine oxidase inhibition [31].…”

Section: Antiproliferationmentioning

confidence: 99%

Flavonoids Nutraceuticals in Prevention and Treatment of Cancer: A Review

Lotha

Sivasubramanian

2018

The objective of the study was to discuss the preventive and treatment of cancer from flavonoids nutraceuticals from our daily dietary source. There has been increasing interest in the research of flavonoids from dietary sources, due to growing evidence of the versatile health benefits of flavonoids through epidemiological studies. Numerous biological activities have been reported. Some clinical trials or meta-analyses have suggested positive associations between flavonoid intake and human health, Several findings have proven that dietary flavonoids to have anticancer properties. Flavonoids due to their nontoxicity in nature and vast, broad aspect of its benefits in biological activities have been intensively studied for their health benefits also added to its abundant availability in our daily diets, for example, green leaves, fruits, red wine, and tea vegetables.