Inhibition of rabies virus infection by a soluble membrane fraction from the rat central nervous system

Conti, Cinzia; Hauttecoeur, Bernard; Morelec, M J; Bizzini, B; Orsi, N; Tsiang, H

doi:10.1007/bf01321007

Cited by 19 publications

(11 citation statements)

References 28 publications

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“…In earlier reports we demonstrated that the lysosomal entry pathway is involved in the infection of non-neuronal cells as well as neurons Tsiang & Superti, 1984). Furthermore highly sialylated gangliosides may participate in these early steps of viral entry into the target cell (Superti et al, 1986;Conti et al, 1988). From our present results we infer that there are presumably two different mechanisms of viral entry into the cell.…”

Section: Retrograde and Anterograde Transport Of Rabies Virus In A Musupporting

confidence: 67%

The Anterograde Transport of Rabies Virus in Rat Sensory Dorsal Root Ganglia Neurons

Tsiang¹,

Lycke²,

Ceccaldi³

et al. 1989

Journal of General Virology

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SUMMARYWe have previously described the capacity of neurites extending from cultured rat sensory dorsal root ganglia (DRG) neurons to transport rabies virus through axoplasm in the retrograde direction. Here we report the infection of cultured neurons derived from the DRG and the subsequent anterograde transport of rabies virus from the infected cell somas through the extending neurites to its release into the culture supernatant. Viral transport was monitored by titration of the virus yield in the external compartment. Both early and late transport mechanisms of rabies virions were identified. The first one occurred a few hours post-infection and was undetectable 6 h later, before the initiation of viral replication. The velocity of this first wave of infective virions was in the range of 100 to 400 mm/day. The early viral transport was probably the result of a direct translocation of infective virions from the somatic site of entry to the neuritic extensions and subsequent release into the culture medium without replication in the cellular perikaryon. The second virus transport peak was detected 48 h post-infection. In this case, the virions detected in the neuritic compartment were presumably the progeny of the inoculated virus which had replicated in the perikaryon before the viral transport occurs. Using a four-compartment culture device we were able to demonstrate, simultaneously, retrograde and anterograde transport of the virus. The presence of antirabies serum in contact with the exposed neurites did not inhibit either the retrograde or the anterograde transport mechanisms. The viral release from the neuritic extensions after the fast anterograde transport was evaluated to be in the range of 150 to 300 infectious virions per bundle of neurites per day.

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Section: Retrograde and Anterograde Transport Of Rabies Virus In A Musupporting

confidence: 67%

The Anterograde Transport of Rabies Virus in Rat Sensory Dorsal Root Ganglia Neurons

Tsiang¹,

Lycke²,

Ceccaldi³

et al. 1989

Journal of General Virology

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“…There is increasing evidence that lipids are present in the cell membrane receptor for many viruses [2,12,16,27] although in the case of Sindbis virus it has been proposed that membrane proteins can represent virus receptors on erythrocytes [21] and B H K cells [4]. The antiviral activity shown by lipids tested here, usually present on the cell surface, can indicate therefore a participation of these substances in a complex cellular receptor structure for Sindbis virus.…”

Section: Discussionmentioning

confidence: 88%

Identification of lipid components of human serum lipoproteins involved in the inhibition of Sindbis virus infectivity, hemagglutination, and hemolysis

Mastromarino

Conti

Rieti

et al. 1988

Archives of Virology

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Human serum high density lipoproteins (HDL), low density lipoproteins (LDL) and very low density lipoproteins (VLDL) were isolated and tested for their ability to inhibit Sindbis virus infectivity, hemagglutination and hemolysis. VLDL and LDL produced a strong reduction on both viral infectivity on Vero cell monolayers and attachment and fusion with erythrocytes, whereas HDL appeared to be only a weak inhibitor. Lipid and protein components were extracted from each class of lipoproteins to identify the molecules responsible for the inhibiting activity. Only the lipid moiety was found to inhibit Sindbis virus biological activities. Among the individual lipid components of lipoproteins, neutral lipids (cholesterol, oleic acid and palmitic acid) and negatively charged phospholipids (phosphatidylserine and phosphatidylinositol) and glycolipids (GM 3 ganglioside and cerebroside sulphate) were able to neutralize the virus suggesting that either hydrophobic or electrostatic interactions are involved in the inhibition.

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“…The isolation and solubilization of cell membranes was performed with a mixture of non-ionic detergents similarly to a preceeding research on rabies virus receptor complex [2]. The chemical nature of the active components was investigated by enzyme treatment of the whole membranes and separation of membrane proteins and lipids.…”

Section: Summary the Chemical Nature Of The Binding Sites For Vesicumentioning

confidence: 99%

“…Rat brain plasma membranes from Wistar rats (3 to 5 weeks old) of both sexes were isolated and solubilized according to the procedure of VandenBerg et al [19] with some modifications [2].…”

Section: Summary the Chemical Nature Of The Binding Sites For Vesicumentioning

confidence: 99%

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Characterization of rat brain cellular membrane components acting as receptors for vesicular stomatitis virus

Conti

Mastromarino

Ciuffarella

et al. 1988

Archives of Virology

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The chemical nature of the binding sites for vesicular stomatitis virus (VSV) was studied by measuring the ability of solubilized rat brain cell membranes (SRBM) to compete with cultured cells for viral binding. SRBM significantly reduced both binding and infectivity of VSV. After separation of protein and lipid components from membranes, VSV infection was unaffected by the protein fraction, whereas the lipid moiety, specifically phospholipids and glycolipids, showed a dose-dependent inhibitory effect. The essential role of phospholipid and sialic acid-containing glycolipid molecules as receptors for VSV was also suggested by the sensitivity of the inhibitory activity of SRBM and lipid components to phospholipase C and neuraminidase digestion.

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Inhibition of rabies virus infection by a soluble membrane fraction from the rat central nervous system

Cited by 19 publications

References 28 publications

The Anterograde Transport of Rabies Virus in Rat Sensory Dorsal Root Ganglia Neurons

The Anterograde Transport of Rabies Virus in Rat Sensory Dorsal Root Ganglia Neurons

Identification of lipid components of human serum lipoproteins involved in the inhibition of Sindbis virus infectivity, hemagglutination, and hemolysis

Characterization of rat brain cellular membrane components acting as receptors for vesicular stomatitis virus

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