Inhibition of porcine reproductive and respiratory syndrome virus by Cecropin D in vitro

Liu, Xiaohong; Guo, Chunhe; Yu-mao, Huang; Zhang, Xiaoyu; Chen, Yaosheng

doi:10.1016/j.meegid.2015.06.021

Cited by 25 publications

(19 citation statements)

References 54 publications

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“…Previous studies have revealed that cecropin CD exhibit potent antiviral activity against PRRSV infection and replication in vitro 41. Cecropin A is suggested to be a novel peptide antibiotic towards multidrug-resistant A. baumannii and P. aeruginosa42.…”

Section: Discussionmentioning

confidence: 98%

“…In recent decades, there is an increasing interest in developing AMPs as natural antibiotics for preventing drug resistance development in infections and probiotic functions 40 . Previous studies have revealed that cecropin CD exhibit potent antiviral activity against PRRSV infection and replication in vitro 41 . Cecropin A is suggested to be a novel peptide antibiotic towards multidrug-resistant A. baumannii and P. aeruginosa 42 .…”

Section: Discussionmentioning

confidence: 99%

“…It has been reported that papiliocin from swallowtail butter fly, Papilio Xuthus, shows high anti-inflammatory activity; and its innate defense response mechanisms engaged by papiliocin involve TLR pathways that culminate in the nuclear translocation of NF-κB 54 . Another finding has shown that interactions between viral particles and receptors on the cell membrane might be blocked by cecropin CD 41 . According to these reports, the inhibitory mechanism of cecropin B on CYP3A29 may indicate a competitive effect for TLRs between pathogen and cecropin B, even though there was no pathogen treatment in the present study.…”

Section: Discussionmentioning

confidence: 99%

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Cecropin B Represses CYP3A29 Expression through Activation of the TLR2/4-NF-κB/PXR Signaling Pathway

Zhou

Wang

et al. 2016

Sci Rep

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Cecropins are peptide antibiotics used as drugs and feed additives. Cecropin B can inhibit the expression of CYP3A29, but the underlying mechanisms remain unclear. The present study was designed to determine the mechanisms responsible for the effects of cecropin B on CYP3A29 expression, focusing on the Toll-like receptors (TLRs) and NF-κB pathways. Our results indicated that the CYP3A29 expression was inhibited by cecropin B, which was regulated by pregnane X receptor (PXR) in a time- and dose-dependent manner. Cecropin B-induced NF-κB activation played a pivotal role in the suppression of CYP3A29 through disrupting the association of the PXR/retinoid X receptor alpha (RXR-α) complex with DNA sequences. NF-κB p65 directly interacted with the DNA-binding domain of PXR, suppressed its expression, and inhibited its transactivation, leading to the downregulation of the PXR-regulated CYP3A29 expression. Furthermore, cecropin B activated pig liver cells by interacting with TLRs 2 and 4, which modulated NF-κB-mediated signaling pathways. In conclusion, cecropin B inhibited the expression of CYP3A29 in a TLR/NF-κB/PXR-dependent manner, which should be considered in future development of cecropins and other antimicrobial peptides.

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Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Cecropin B Represses CYP3A29 Expression through Activation of the TLR2/4-NF-κB/PXR Signaling Pathway

Zhou

Wang

et al. 2016

Sci Rep

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“…Some AMPs including Cecropin D (CD-PRRSV, SALPs-AIV) were reported to have blocked viral attachment and invasion by interacting with the receptors of the host cells [ 32 ]. Based on these findings, so we tried to keep PEDV infection within the periods of the attachment and entry through the temperature control, and the results showed no evident differences in protein expression among attachment period treated group, entry period treated group, and the PEDV control group.…”

Section: Discussionmentioning

confidence: 99%

Caerin1.1 Suppresses the Growth of Porcine Epidemic Diarrhea Virus In Vitro via Direct Binding to the Virus

Guo

Zhang

et al. 2018

Viruses

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Porcine epidemic diarrhea (PED) has re-emerged in recent years and has already caused huge economic losses to the porcine industry all over the world. Therefore, it is urgent for us to find out efficient ways to prevent and control this disease. In this study, the antiviral activity of a cationic amphibian antimicrobial peptide Caerin1.1 against porcine epidemic diarrhea virus (PEDV) was evaluated by an in vitro system using Vero cells. We found that even at a very low concentration, Caerin1.1 has the ability to destroy the integrity of the virus particles to block the release of the viruses, resulting in a considerable decrease in PEDV infections. In addition, Caerin1.1 showed powerful antiviral activity without interfering with the binding progress between PEDV and the receptor of the cells, therefore, it could be used as a potential antiviral drug or as a microbicide compound for prevention and control of PEDV.

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“…Prevention efforts include strict biosecurity protocols to prevent introduction of the virus to a farm (Dee et al, 2003;Dee et al, 2004;Dee et al, 2010;Alonso et al, 2013) and vaccination against the PRRSV, which provides little protection, if any (Bourry et al, 2015;Kim et al, 2015;Lyoo, 2015;Rose et al, 2015). Methods used to resolve the PRRSV infection include veterinarian treatment through administration of antiviral medication (Liu et al, 2015) or other compounds (Sun et al, 2014), or a complete depopulation and repopulation of the infected farm . PRRSV infected pigs have poorer growth rates than uninfected pigs (Mengeling et al, 1998;M.M.…”

Section: Introductionmentioning

confidence: 99%

Molecular and quantitative genetic basis and control of severe combined immunodeficiency and porcine reproductive and respiratory syndrome in pigs

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First and foremost, I would like to acknowledge the love, support, and guidance of my family; without you all, I would have never made it to where I am today. Mama, you are my confidant, therapist, and most importantly, my mama. I will forever be grateful for everything that you have done for me since the day I was born. Your selflessness is endless. Dad, thank you for your guidance, advice, and help through the years. John and Ben, thank you both for always putting a smile on my face, even when times are tough. Your confidence in me is something that holds a very special place in my heart. To my sweet sister, Nancy, even though you are no longer here, the love that we shared is constantly with me. My memories of you have gotten me through the worst struggles, and I hope that you are proud of the woman I have become since we last saw one another. I would like to thank my advisors and their wives. Dr. Jack Dekkers, I will never forget our first meeting for breakfast when I came to interview. You were so wise, so accepting, so honest. I'm very glad that you didn't tell me how cold it would be in January when I moved to Iowa (it was much warmer when I interviewed in June). Your unwavering calm demeanor has taught me so much in the years since our first meeting. You are such an admirable man. Thank you for your academic advising, as well as personal. Mrs. Sue Dekkers, I'm not going to go too much into our first meeting, road-tripping to Chicago and refusing to touch Jack's new iPhone after Nick Boddicker nicked it up. But, I will say that your bright personality has brought many smiles to my face! You are a wonderful GradMa to all of us. I've enjoyed our times spent together. Dr. Chris Tuggle, I tear up just trying to think of what to say to thank you for everything. I hope that one day, I will have half of the finesse that you exhibit daily. You are such a deep scientific thinker, yet you never fail to notice the most subtle emotional change. I am xi eternally grateful for the time I've spent in your office, and I believe I may still have a box of your facial tissues at my desk. Thank you for picking me up when I was down and for your confidence in me. Mrs. Maureen Tuggle, you are such a kind spirit, always thoughtful and willing to lend a helping hand no matter the circumstances. Your kindness towards me has taught me the importance of grace and love. I have such great admiration and respect for all of you. And to all four of you, I would get in the hot tub with you now. I would also like to show my appreciation for my committee members for their assistance and guidance through my graduate studies. Dr. Joan Cunnick, thank you for all of your help with the endless immunology questions that have come along with the SCID project. Dr. Peng Liu, thank you for sharing your knowledge of statistics with me. You are such a wonderful teacher. Dr. Dorian Garrick, I am very grateful for all of our interactions. You have taught me so much about the animal breeding industry, and I know that will serve me well throughout my caree...

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Inhibition of porcine reproductive and respiratory syndrome virus by Cecropin D in vitro

Cited by 25 publications

References 54 publications

Cecropin B Represses CYP3A29 Expression through Activation of the TLR2/4-NF-κB/PXR Signaling Pathway

Cecropin B Represses CYP3A29 Expression through Activation of the TLR2/4-NF-κB/PXR Signaling Pathway

Caerin1.1 Suppresses the Growth of Porcine Epidemic Diarrhea Virus In Vitro via Direct Binding to the Virus

Molecular and quantitative genetic basis and control of severe combined immunodeficiency and porcine reproductive and respiratory syndrome in pigs

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