Inhibition of neutrophil function by hydrogen peroxide

Rajkovic, I. A.

doi:10.1016/0006-2952(85)90399-5

Cited by 16 publications

(10 citation statements)

References 16 publications

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“…2) probably indicated a fraction of pmn luminol CL generated extracellularly. With a low affinity for H 202 (22), CAT deals poorly (Fig. 2) with the low extracellular peroxide levels that may follow PMA stimulation.…”

Section: Discussionmentioning

confidence: 99%

Myeloperoxidase‐Based Chemiluminescence of Polymorphonuclear Leukocytes and Monocytes

McNally

Bell

1996

J. Biolumin. Chemilumin.

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Luminol and lucigenin chemiluminescence (CL) responses produced by separated human blood polymorphonuclear leukocytes (pmn) and monocytes (mono) have been studied following stimulation with the surface-receptor agonist fMLP (a synthetic chemotactic peptide) and the protein kinase C activator phorbol myristate acetate (PMA). Pmn produced two- to threefold the luminol CL and superoxide anion (O2-) levels of mono; lucigenin CL was similar for both cell-types. The myeloperoxidase (MPO) inhibitor salicylhydroxamic acid (SHA) abrogated luminol but not lucigenin CL in both cell types, but did not further inhibit the already grossly subnormal luminol CL responses seen with MPO-deficient cells which produced normal lucigenin CL. SHA also profoundly inhibited the luminol CL response in a cell-free MPO-H2O2 system. Mono lucigenin CL does not appear to specifically measure O2- production. These data show that luminol CL provides a useful measure of pmn and also mono MPO activity. However, analysis of the effects of various reactive oxygen species (ROS) scavengers, assessed on phagocyte and cell-free CL systems (both MPO-H2O2 and superoxide generating) suggest that the luminol CL signal is not entirely dependent on MPO activity.

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Section: Discussionmentioning

confidence: 99%

Myeloperoxidase‐Based Chemiluminescence of Polymorphonuclear Leukocytes and Monocytes

McNally

Bell

1996

J. Biolumin. Chemilumin.

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“…When glutathione availability in the epithelial lining fluid is limited, the phagocytosis, the respiratory burst, and microbial clearance are compromised (Rajkovic and Williams, 1985;Seres et al, 2000). Alcohol abuse independently increases the risk and severity of acute respiratory distress syndrome (Moss et al, 1996(Moss et al, , 1999 and decreases glutathione availability and subsequent oxidant stress in the epithelial lining fluid (Moss et al, 2000).…”

Section: Lou Ann S Brownmentioning

confidence: 99%

Acute and Chronic Alcohol Abuse Modulate Immunity

Brown

Cook

Jerrells

et al. 2006

Alcoholism Clin & Exp Res

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This article represents the proceedings of the Alcohol and Immunology Research Interest Group (AIRIG) meeting, a satellite workshop held at the 37th Annual Meeting of the Society for Leukocyte Biology. The meeting was sponsored by the AIRIG and the National Institute on Alcohol Abuse and Alcoholism. The presentations were as follows: (1) Effects of Ethanol on Immune Response to Hepatitis C Virus by Jack R. Wands, (2) Alcohol and Alveolar Macrophage Dysfunction: The Role of Chronic Oxidant Stress by Lou Ann S. Brown, (3) T Cell Responses to Listeria monocytogenes in Mice on a Chronic Ethanol Exposure Protocol by Robert T. Cook, (4) Mechanisms of Acute and Chronic Alcohol Consumption on Severity of Viral Infections by the Liver and Pancreas by Thomas R. Jerrells, (5) Acute and Chronic Effects on Macrophage Ectodomain Shedding: Implications for Lung Host Defenses by Jay K. Kolls, (6) Increased Susceptibility to Pseudomonas Infection of Burn-Injured Mice Given Alcohol Before Injury by Elizabeth J. Kovacs, (7) Regulation of Tumor Necrosis Factor alpha Expression in Macrophages by Chronic Ethanol by Laura E. Nagy, and (8) Hepatitis C Virus Infection and Alcohol Use by Gyongyi Szabo. Meeting coorganizers were Elizabeth J. Kovacs, Lou Ann S. Brown, Thomas R. Jerrells, and Robert T. Cook.

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“…Among blood cells, GSH has been most studied in erythrocytes (1)(2)(3)(4), and it has been investigated to a lesser extent in leukocytes of mice (5) and humans (6,7). The lack of studies of leukocytes can in part be ascribed to the relative difficulty involved in the effective separation of the different leukocyte types, the relative paucity of monocytes among the more prevalent neutrophils and lymphocytes, and the general lack of understanding of the physiologic role of GSH in these cells.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous measurement of neutrophil, lymphocyte, and monocyte glutathione by flow cytometry

Scott

Collins

Matin

et al. 1990

Clinical Laboratory Analysis

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A flow cytometric method for quantitation of glutathione (GSH) was applied to simultaneous analysis of the major leukocyte types in peripheral blood. Cellular thiols (predominantly GSH) were stained with monochlorobimane (MCIB), and thiol fluorescence was measured with a flow cytometer. The fluorescence of the thiols closely reflected the GSH content, as measured by a specific glutathione reductase assay. Fluorescence of individual cell types could be measured after delineating those cells by their light-scatter characteristics, utilizing dual-angle light scatter for discrimination. By this means, GSH contents of 12.5 +/- 2.0 nmol/10(7) neutrophils, 14.5 +/- 2.7 nmol/10(7) monocytes, and 5.0 +/- 1.0 nmol/10(7) lymphocytes were found. The results obtained for neutrophils with the flow cytometer were virtually identical with those obtained with chemical assay in purified samples of neutrophils, indicating the validity of the flow cytometric method.

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Inhibition of neutrophil function by hydrogen peroxide

Cited by 16 publications

References 16 publications

Myeloperoxidase‐Based Chemiluminescence of Polymorphonuclear Leukocytes and Monocytes

Myeloperoxidase‐Based Chemiluminescence of Polymorphonuclear Leukocytes and Monocytes

Acute and Chronic Alcohol Abuse Modulate Immunity

Simultaneous measurement of neutrophil, lymphocyte, and monocyte glutathione by flow cytometry

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