Myeloperoxidase‐Based Chemiluminescence of Polymorphonuclear Leukocytes and Monocytes

McNally, J. A.; Bell, Andrew S.

doi:10.1002/(sici)1099-1271(199603)11:2<99::aid-bio404>3.3.co;2-v

Cited by 9 publications

(7 citation statements)

References 16 publications

(19 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These results suggest that NADPH oxidase activity may be suppressed after the camp. First-and second-order reactions with respect to extracellular superoxide concentrations have been proposed as mechanisms of Cyt.c and LgCL respectively [13]. The discrepancies may be caused by differences between the reaction mechanisms of these measurements.…”

Section: Discussionmentioning

confidence: 99%

Effects of Summer Camp Endurance Training on Non-Specific Immunity in Long-Distance Runners

Kumae

Yamasaki²,

Ishizaki³

et al. 1999

Int J Sports Med

View full text Add to dashboard Cite

The present study was conducted to investigate the effects of endurance training during a 1-month summer camp on the non-specific host defence systems of ten highly trained male long-distance runners. Examinations with informed consent were carried out three times: 2 days before the training began, an intermediate day, and 2 days after the camp. The subjects ran a total of about 800 km during the camp. Neutrophilic reactive oxygen species (ROS) generation determined by luminol- and lucigenin-dependent chemiluminescence and cytochrome c reduction methods was assayed at two time points because the camp was held far from our laboratory: neutrophils isolated 6 hours after blood sampling were examined at all three time points and neutrophils isolated immediately after blood sampling were examined before and after the camp. The ROS production indicated by peak height of luminol-dependent chemiluminescence and peak velocity of cytochrome c reduction from neutrophils isolated 6 hours after blood sampling was suppressed after the camp (P < 0.05 for both), but, the ROS production from neutrophils isolated immediately after blood sampling and the serum opsonic activity appeared not to be affected. Neutrophils diminished their activity during the 6-hour waiting time after the camp. These results suggest that the non-specific host defence system is not directly impaired by long, strenuous endurance training.

show abstract

Section: Discussionmentioning

confidence: 99%

Effects of Summer Camp Endurance Training on Non-Specific Immunity in Long-Distance Runners

Kumae

Yamasaki²,

Ishizaki³

et al. 1999

Int J Sports Med

View full text Add to dashboard Cite

show abstract

“…24 27 34 It has been shown that myeloperoxidase activity is also required for the luminol reaction, together with H 2 O 2 that is generated by the respiratory burst. [35][36][37] Isoluminol, however, does not penetrate neutrophils and so can be used to measure only reactive oxidants that are released from activated neutrophils. 31 32 Isoluminol was therefore used as a probe to determine extracellular release of reactive oxidants.…”

Section: Activation Of Primed and Unprimed Neutrophils By Immune Compmentioning

confidence: 99%

Insoluble and soluble immune complexes activate neutrophils by distinct activation mechanisms: changes in functional responses induced by priming with cytokines

Fossati

Bucknall²,

Edwards³

2002

Annals of the Rheumatic Diseases

View full text Add to dashboard Cite

Background: Rheumatoid synovial fluid contains both soluble and insoluble immune complexes that can activate infiltrating immune cells such as neutrophils. Objectives: To determine if these different complexes activate neutrophils through similar or different receptor signalling pathways. In particular, to determine the circumstances which result in the secretion of tissue damaging reactive oxygen metabolites and granule enzymes. Methods: Blood neutrophils were incubated with synthetic soluble and insoluble immune complexes and the ability to generate reactive oxidants tested by luminescence or spectrophotometric assays that distinguished between intracellular and extracellular production. Degranulation of myeloperoxidase and lactoferrin was determined by western blotting. The roles of FcγRII (CD32) and FcγRIIIb (CD16) were determined by incubation with Fab/F(ab′) 2 fragments before activation. The effect of cytokine priming was determined by incubation with GM-CSF. Results: Insoluble immune complexes activated unprimed neutrophils, but most of the oxidants produced were intracellular. This activation required FcγRIIIb, but not FcγRII function. Soluble complexes failed to activate unprimed neutrophils but generated a rapid and extensive secretion of reactive oxygen metabolites when the cells were primed with granulocyte-macrophage colony stimulating factor (GM-CSF). This activity required both FcγRII and FcγRIIIb function. Insoluble immune complexes activated the release of granule enzymes from primed or unprimed neutrophils, but the kinetics of release did not parallel those of secretion of reactive oxygen metabolites. Only primed neutrophils released enzymes in response to soluble complexes. Conclusions: Soluble and insoluble immune complexes activate neutrophils by separate receptor signalling pathways. Profound changes in neutrophil responsiveness to these complexes occur after cytokine priming.

show abstract

“…Luminol specifically measures H 2 O 2 generated by superoxide dismutase (SOD) when horseradish peroxidase (HRP) in excess represses the production of all subsequent ROS (16,17). In the absence of HRP, it measures all the ROS with a sensitivity greatest for the ROS generated by the myeloperoxidase (MPO)-H 2 O 2 -Cl 2 system (18,19). Since luminol enters the cell, it also reflects intracellular ROS production (20).…”

Section: Introductionmentioning

confidence: 99%

Inhibition of Chemiluminescence in Human PMNs by Monocyclic Phenolic Acids and Flavonoids

Limasset

Ojasoo²,

Doucen³

et al. 1999

Planta med

View full text Add to dashboard Cite

Flavonoids are metabolized in vivo to monocyclic phenolic acids. We investigated whether 18 phenolic acids of the benzoic, phenylacetic, phenylpropanoic or cinnamic series-known or potential metabolites of flavonoids-inhibit reactive oxygen species (ROS) released by human polymorphonuclear neutrophils (PMNs). Chemiluminescence was measured after PMN stimulation with three agents (N-fMetLeuPhe, phorbol myristate acetate (PMA), or opsonised zymosan) using two probes (lucigenin or luminol) with or without horseradish peroxidase (HRP) in order to derive specificity profiles for each test compound. The profiles of the phenolic acids and flavonoids were compared by a multivariate (correspondence) factor analysis. Overall, the phenolic acids were less specific than the flavonoids and, with a few exceptions, less potent. Phenolic acids had virtually no effect on the chemiluminescence related to O2- formation that is measured by lucigenin but inhibited luminol luminescence. Inhibition for all but two phenolic acids was sensitive to HRP and might be explained by a scavenger mechanism. Few structure-activity relationships emerged suggesting that simple properties such as radical scavenging and/or redox activity rather than overall structure might be the key determinants of chemiluminescence inhibition. Whatever the mechanism, however, we conclude that part of the in vivo pharmacological activity of flavonoids may readily be accounted for by phenolic acids.

show abstract

Myeloperoxidase‐Based Chemiluminescence of Polymorphonuclear Leukocytes and Monocytes

Cited by 9 publications

References 16 publications

Effects of Summer Camp Endurance Training on Non-Specific Immunity in Long-Distance Runners

Effects of Summer Camp Endurance Training on Non-Specific Immunity in Long-Distance Runners

Insoluble and soluble immune complexes activate neutrophils by distinct activation mechanisms: changes in functional responses induced by priming with cytokines

Inhibition of Chemiluminescence in Human PMNs by Monocyclic Phenolic Acids and Flavonoids

Contact Info

Product

Resources

About