Inhibition of hCG-Stimulated Steroidogenesis in Cultured Mouse Leydig Tumor Cells by Bisphenol A and Octylphenols

Nikula, Hannu; Talonpoika, T.; Kaleva, Marko; Toppari, Jorma

doi:10.1006/taap.1999.8674

Cited by 74 publications

(45 citation statements)

References 38 publications

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“…The study by Akingbemi et al (2004) should nevertheless be considered for mechanistic value, and is considered adequate but of limited utility by the Panel for the evaluation process. Nikula et al (1999), support not indicated, examined the in vitro effects of bisphenol A on steroidogenesis in mouse Leydig tumor cell cultures. Octyl phenols were also examined in this study, but results will not be discussed.…”

Section: Utility (Adequacy) For Cerhr Evaluation Processmentioning

confidence: 99%

NTP‐CERHR expert panel report on the reproductive and developmental toxicity of bisphenol A

Chapin

Adams

Boekelheide

et al. 2008

Birth Defects Research Pt B

401

304

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Section: Utility (Adequacy) For Cerhr Evaluation Processmentioning

confidence: 99%

NTP‐CERHR expert panel report on the reproductive and developmental toxicity of bisphenol A

Chapin

Adams

Boekelheide

et al. 2008

Birth Defects Research Pt B

401

304

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“…BPA has been reported to have estrogenic actions such as uterotrophic effects [3], decreased sperm production [4,5], stimulation of prolactin release [6], promotion of cell proliferation in a breast cancer cell line [7], and influence on preimplantation development [8], by animal experiments and in vitro studies. It was also reported that the activities of BPA depended on the mechanism via estrogen receptors (ERa and ERb) [9,10].…”

mentioning

confidence: 99%

Positive Relationship between Androgen and the Endocrine Disruptor, Bisphenol A, in Normal Women and Women with Ovarian Dysfunction

et al. 2004

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Abstract. This study was performed to investigate the serum levels of bisphenol A (BPA), an endocrine disruptor, in women with ovarian dysfunction and obesity. Fasting serum samples were obtained from 19 non-obese and 7 obese women with normal menstrual cycles: 7 patients with hyperprolactinemia, 21 patients with hypothalamic amenorrhea, and 13 non-obese and 6 obese patients with polycystic ovary syndrome (PCOS). BPA was measured by an enzyme-linked immunosorbent assay. BPA was detected in all human sera. Serum BPA concentrations were significantly higher in both non-obese and obese women with polycystic ovary syndrome (1.05 ± 0.10 ng/ml, 1.17 ± 0.16 ng/ml; p<0.05, respectively) and obese normal women (1.04 ± 0.09 ng/ml, p<0.05) compared with those in non-obese normal women (0.71 ± 0.09 ng/ ml). There was no difference among women with hyperprolactinemia, women with hypothalamic amenorrhea, and nonobese normal women. There were significant positive correlations between serum BPA and total testosterone (r = 0.391, p<0.001), free testosterone (r = 0.504, p<0.001), androstenedione (r = 0.684, p<0.001), and DHEAS (r = 0.514, p<0.001) concentrations in all subjects. These findings show that there is a strong relationship between serum BPA and androgen concentrations, speculatively due to the effect of androgen on the metabolism of BPA.

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“…This reaction is related to intracellular Ca +2 mobilization (Mennon and Mennon 2012). A similar effect has been observed on E2 secretion in the mTLC-1 cell line when treated with the estrogen-like substance bisphenol A after an FSH surge (Nikula et al 1999). Moreover, bisphenol A and another estrogen-like chloroorganic insecticide, DDT, have been shown to decrease the cAMP concentration in cells (Nikula et al 1999, Pohland and Tiemann 2003, Wójtowicz et al 2007.…”

Section: Discussionmentioning

confidence: 62%

“…Moreover, certain individual congeners impair the effect of FSH-stimulation on OT secretion from bovine granulosa cells and suppress the effects of LH on progesterone (P4) and OT secretion from bovine luteal cells (Mlynarczuk and Kotwica 2006). The mechanism underlying this effect in not known, but it has been suggested that xenobiotics may disrupt the signals transmission, generated from membrane receptors (Nikula et al 1999). Additionally, PCBs can disturb the intracellular concentration of Ca +2 in human granulocytes (Voie and Fonnum 1998), decrease the concentrations of cAMP and phosphoinositols concentrations in cortical cells (Inglefield et al 2001) and decrease intracellular calcium mobilization in bovine myometrial cells (Bae et al 1999, Wrobel and and mouse thymocytes (Yilmaz et al 2006).…”

Section: Introductionmentioning

confidence: 99%

The effect of PCB126, 77, and 153 on the intracellular mobilization of Ca+2 in bovine granulosa and luteal cells after FSH and LH surge in vitro

Mlynarczuk¹,

Kowalik²

2013

Polish Journal of Veterinary Sciences

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Polychlorinated biphenyls (PCBs) are a group of persistent environmental pollutants that impair cattle reproduction. Among other effects, PCBs can disturb the intracellular mobilization of Ca +2 in several cell types. Hence, it is possible that they disrupt the transduction of intracellular signals generated from gonadotropin (FSH/LH) receptors. In steroidogenic ovarian cells, a defect in Ca +2 mobilization may have a detrimental influence on two important processes: the secretion of steroids (E2 or/and P4) and their morphological and functional differentiation. The aim of this study was to determine the influence of PCBs: 126 (dioxin-like) 77 (ambivalent) and 153 (estrogen-like) and a mixture of PCBs (Aroclor 1248) on these processes. Bovine granulosa and luteal cells were incubated for 72 hrs with PCBs (100 ng/ml), followed by Fura 2AM dye, and the fluctuations in intracellular Ca +2 mobilization after FSH/LH treatment were determined using an inverted microscope coupled with a CCD camera. The intensity and area of fluorescence excited by UV light were detected in the green spectrum of visible light. Aroclor 1248 and PCBs 153 and 77 significantly decreased (P < 0.01-0.001) the effect of FSH on intracellular Ca +2 mobilization in granulosa cells. In luteal cells, the most effective PCB on this process was PCB 77. The results revealed adverse effects of PCBs on the mobilization of intracellular Ca +2. Moreover, the estrogen-like congeners were found to more effectively disturb this process than the dioxin-like PCB 126. Hence, it is possible for PCBs to have a negative influence on reproductive processes by affecting calcium mobilization.

show abstract

Inhibition of hCG-Stimulated Steroidogenesis in Cultured Mouse Leydig Tumor Cells by Bisphenol A and Octylphenols

Cited by 74 publications

References 38 publications

NTP‐CERHR expert panel report on the reproductive and developmental toxicity of bisphenol A

NTP‐CERHR expert panel report on the reproductive and developmental toxicity of bisphenol A

Positive Relationship between Androgen and the Endocrine Disruptor, Bisphenol A, in Normal Women and Women with Ovarian Dysfunction

The effect of PCB126, 77, and 153 on the intracellular mobilization of Ca+2 in bovine granulosa and luteal cells after FSH and LH surge in vitro

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