The effect of PCB126, 77, and 153 on the intracellular mobilization of Ca+2 in bovine granulosa and luteal cells after FSH and LH surge in vitro

Abstract

Polychlorinated biphenyls (PCBs) are a group of persistent environmental pollutants that impair cattle reproduction. Among other effects, PCBs can disturb the intracellular mobilization of Ca +2 in several cell types. Hence, it is possible that they disrupt the transduction of intracellular signals generated from gonadotropin (FSH/LH) receptors. In steroidogenic ovarian cells, a defect in Ca +2 mobilization may have a detrimental influence on two important processes: the secretion of steroids (E2 or/and P4) and their morphological and functional differentiation. The aim of this study was to determine the influence of PCBs: 126 (dioxin-like) 77 (ambivalent) and 153 (estrogen-like) and a mixture of PCBs (Aroclor 1248) on these processes. Bovine granulosa and luteal cells were incubated for 72 hrs with PCBs (100 ng/ml), followed by Fura 2AM dye, and the fluctuations in intracellular Ca +2 mobilization after FSH/LH treatment were determined using an inverted microscope coupled with a CCD camera. The intensity and area of fluorescence excited by UV light were detected in the green spectrum of visible light. Aroclor 1248 and PCBs 153 and 77 significantly decreased (P < 0.01-0.001) the effect of FSH on intracellular Ca +2 mobilization in granulosa cells. In luteal cells, the most effective PCB on this process was PCB 77. The results revealed adverse effects of PCBs on the mobilization of intracellular Ca +2. Moreover, the estrogen-like congeners were found to more effectively disturb this process than the dioxin-like PCB 126. Hence, it is possible for PCBs to have a negative influence on reproductive processes by affecting calcium mobilization.

Polish Journal of Veterinary Sciences

“…Polychlorinated biphenyls (PCBs) and pesticide DDT as well as its metabolite DDE are resistant to biodegradation (Borja et al 2005), and due to their lipophilic properties, they can accumulate in tissues of living organisms (Glynn et al 2000). These environmental pollutants can act as agonists or antagonists of gonadal steroid hormones (Goldman and Yawetz 1992, Safe 1994, Schrader et al 2003, influence oxytocin (OT) secretion (Mlynarczuk et al 2009, Wrobel et al 2014, and decrease the sensitivity of granulosa and luteal cells to gonadotropins (Mlynarczuk and Kowalik 2013). We observed that these xenobiotics (XBs) in concentrations detected in the reproductive tract (Kamarianos et al 2003, Mlynarczuk et al 2005) disturb the secretory function of the ovaries and both contractions and secretory functions of the uterus and oviducts during the estrus cycle and pregnancy (Mlynarczuk et al 2009, Wrobel et al 2010.…”

Evidence for direct effects of glyphosate on ovarian function: glyphosate influences steroidogenesis and proliferation of bovine granulosa but not theca cellsin vitro

“…Thus, the present study aimed to determine whether GLY directly affects ovarian function. To address this aim, bovine granulosa cells (GC) and theca cells (TC), which represent an intriguing in vitro model to study contaminant reproductive effects (Mlynarczuk & Kowalik, ; Mlynarczuk et al ., ; Petro et al ., ; Pizzo et al ., ,b; Spicer & Aad, ) were used and the effects of GLY on ovarian cell proliferation, steroid production and gene expression were evaluated.…”