Inhibition of Escherichia coli O157:H7 and Salmonella enterica virulence factors by benzyl isothiocyanate

Patel, Jitendra; Yin, Hsin‐Bai; Bauchan, Gary R.; Mowery, Joseph

doi:10.1016/j.fm.2019.103303

Cited by 31 publications

(16 citation statements)

References 33 publications

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“…The BIT along with allyl, 2‐phenylethyl and phenyl isothiocyanates have also been used to control Alternaria rot disease in packaged bell peppers (Troncoso et al , ). Our previous study demonstrated the mechanisms of actions of BIT against Salmonella , where the disruption of outer membrane, release of cytoplasmic contents and cell lysis of S. Braenderup occurred following the BIT treatment in vitro (Patel et al , ).…”

Section: Discussionmentioning

confidence: 97%

“…Glucosinolates are natural plant compounds present in the cruciferous vegetables such as cauliflower, broccoli and cabbage (Keck & Finley, ). Antimicrobial activities of glucosinolate hydrolysis compounds that are released during enzymatic reaction of myrosinase have been previously demonstrated in vitro (Aires et al , ; Patel et al , ). Jang et al () reported the antimicrobial activities of isothiocyanates (butenyl, pentenyl, phenylethyl and benzyl), which are a group of glucosinolate hydrolysis compounds, against pathogenic bacteria including L. monocytogenes , Vibrio parahaemolyticus , Staphylococcus aureus and Salmonella enterica by agar diffusion assay.…”

Section: Introductionmentioning

confidence: 98%

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Efficacy of benzyl isothiocyanate for controlling Salmonella on alfalfa seeds and sprouts

Yin

Boomer

Chen

et al. 2020

Int J of Food Sci Tech

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The efficacy of benzyl isothiocyanate (BIT), a compound found in cruciferous vegetables, for controlling Salmonella on alfalfa seeds and sprouts was investigated. Alfalfa seeds were inoculated by individual Salmonella enterica serotypes Newport, Typhimurium, Tennessee, Montevideo, or Braenderup at two inoculation levels of 3 or 5 log CFU g À1 , followed by treatment with BIT (0.5-2%) or chlorine (2%) for 15 min. Treated seeds were sprouted in seed sprouters. Surviving Salmonella populations on the seeds and sprouts were determined. At both inoculum levels, 1.5% and 2% of BIT significantly reduced populations S. Typhimurium and S. Braenderup on seeds to a level comparable to the chlorine-treated seeds. Salmonella populations recovered from the sprouts germinated from 1.5% or 2% BIT-treated seeds were significantly lower than the control and chlorine-treated seeds at both inoculum levels (P < 0.05). The results of the study indicate that BIT can be used to control Salmonella contamination on alfalfa seeds and subsequent sprouting process.

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Section: Discussionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 98%

Efficacy of benzyl isothiocyanate for controlling Salmonella on alfalfa seeds and sprouts

Yin

Boomer

Chen

et al. 2020

Int J of Food Sci Tech

Self Cite

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“…This highlights that BITC is a good natural product for inhibiting S. typhimurium. Moreover, it has been proven by a large number of experimental studies that the antibacterial effect of BITC against foodborne pathogens is of great significance for food safety and clinical research [12,[28][29][30].…”

Section: Discussionmentioning

confidence: 99%

“…When the concentration was equal to the MIC, the motility of S. typhimurium was completely inhibited. Jitendra et al [28] have indicated that 125 µg/mL BITC for S. typhimurium and 150 µg/mL BITC for E. coli O157:H7 can significantly inhibit the motility of both bacteria. Selwan et al, and Smriti et al, [39,40] have shown that the motility regulated by flagella is significantly different when the expression of flagella system-related genes is changed.…”

Section: Discussionmentioning

confidence: 99%

Transcriptomic Analysis, Motility and Biofilm Formation Characteristics of Salmonella typhimurium Exposed to Benzyl Isothiocyanate Treatment

Niu

Wang

et al. 2020

IJMS

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Salmonella typhimurium (S. typhimurium) is a common foodborne pathogen that not only causes diseases and contaminates food, but also causes considerable economic losses. Therefore, it is necessary to find effective and feasible methods to control S. typhimurium. In this study, changes in S. typhimurium after treatment with benzyl isothiocyanate (BITC) were detected by transcriptomics to explore the antibacterial effect of BITC at subinhibitory concentration. The results showed that, in contrast to the control group (SC), the BITC-treated group (SQ_BITC) had 197 differentially expressed genes (DEGs), of which 115 were downregulated and 82 were upregulated. We screened out eight significantly downregulated virulence-related genes and verified gene expression by quantitative Real-time Polymerase Chain Reaction (qRT-PCR). We also selected motility and biofilm formation to observe the effects of BITC on the other virulence related factors of S. typhimurium. The results showed that both swimming and swarming were significantly inhibited. BITC also had a significant inhibitory effect on biofilm formation, and showed an effect on bacterial morphology. These results will be helpful for understanding the mechanism of the antibacterial action of BITC against S. typhimurium and other foodborne pathogens.

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“…Over the past decade, the use of plant-derived compounds as alternative antimicrobials has gained signi cant attention as a result of increasing concerns over the safety of synthetic antimicrobial agents and the emergence of antibiotic-resistant bacteria [27]. Coenzyme Q 0 (CoQ 0 ; 2,3-dimethoxy-5-methyl-1,4benzoquinone; C 9 H 10 O 4 ) is a benzoquinone compound extracted from the fungus Antrodia camphorata [28].…”

Section: Introductionmentioning

confidence: 99%

Inhibition of Cronobacter Sakazakii Biofilm Formation and Expression of Virulence Factors by Coenzyme Q0

Guan¹,

Shi²,

Tong³

et al. 2020

Preprint

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Objectives：Here, we investigated the inhibitory effects of coenzyme Q0 (CoQ0) on biofilm formation and the expression of virulence genes by Cronobacter sakazakii. Results：We found that the minimum inhibitory concentration of CoQ0 against C. sakazakii strains ATCC29544 and ATCC29004 was 100 μg/mL, while growth curve assays showed that sub-inhibitory concentrations (SICs) of CoQ0 for both strains were 6.4, 3.2, 1.6 and 0.8 μg/mL. Assays exploring the inhibition of specific biofilm formation showed that SICs of CoQ0 inhibited biofilm formation by C. sakazakii in a dose-dependent manner, which was confirmed by scanning electron microscopy and confocal laser scanning microscopy analyses. CoQ0 inhibited the swimming and swarming motility of C. sakazakii and reduced its ability to adhere to and invade HT-29 cells. In addition, CoQ0 impeded the ability of C. sakazakii to survive and replicate within RAW 264.7 cells. Finally, real time polymerase chain reaction analysis confirmed that nine C. sakazakii genes associated with biofilm formation and virulence were down-regulated in response to CoQ0 treatment. Conclusion：Overall, our findings suggest that CoQ0 is a promising antibiofilm agent and provide new insights for the prevention and control of infections caused by C. sakazakii.

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Inhibition of Escherichia coli O157:H7 and Salmonella enterica virulence factors by benzyl isothiocyanate

Cited by 31 publications

References 33 publications

Efficacy of benzyl isothiocyanate for controlling Salmonella on alfalfa seeds and sprouts

Efficacy of benzyl isothiocyanate for controlling Salmonella on alfalfa seeds and sprouts

Transcriptomic Analysis, Motility and Biofilm Formation Characteristics of Salmonella typhimurium Exposed to Benzyl Isothiocyanate Treatment

Inhibition of Cronobacter Sakazakii Biofilm Formation and Expression of Virulence Factors by Coenzyme Q0

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