Inhibition of energy metabolism by 3-bromopyruvate in the hard tick Rhipicephalus microplus

Braz, Valdir; Gomes, Helga; Galina, Antônio; Saramago, Luiz; Braz, Glória R.C.; Vaz, Itabajara da Silva; Logullo, Carlos; Fonseca, Rodrigo Nunes da; Campos, Eldo; Moraes, João Antônio de

doi:10.1016/j.cbpc.2018.12.010

Cited by 3 publications

(12 citation statements)

References 39 publications

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“…In R. microplus , this enzyme is found on cytosol or in the outer membrane of mitochondria. The inhibition of hexokinase can cause deleterious effects on the tick, such as the production of reactive oxygen species (ROS) and reduction of ATP levels (Fraga et al ., 2013; Braz et al ., 2019). Then, the enzymes involved in energetic metabolism during embryogenesis represent potential molecular targets for parasitic control, since they may interfere with embryonic development and consequently interrupt the life cycle of the tick (Logullo et al ., 2009).…”

Section: Resultsmentioning

confidence: 99%

“…The authors also reported that BmTIM shows high sensitivity to the action of the thiol reagents dithionitrobenzoic acid and mMMTS. TIM inhibition can promote an increase in the formation of methylglyoxal (intermediary metabolite of the reaction catalysed by TIM), a harmful molecule for ticks (Braz et al ., 2019).…”

Section: Resultsmentioning

confidence: 99%

“…The respiratory chain consists of four multi-enzymatic protein complex and electron carriers, such as Q co-enzyme and cytochrome c. Among these enzymes, the complex NADH cytochrome c reductase and succinate cytochrome c reductase stands out, which participates in important stages of ATP production. Mitochondrial respiratory chain changes are related to the increase in intracellular levels of ROS, which can compromise the development of ticks at different stages (Braz et al ., 2019).…”

Section: Resultsmentioning

confidence: 99%

“…Variations in glucose and glycogen levels during R. microplus embryogenesis are related to glycolysis and gluconeogenesis (Moraes et al, 2007). In the first stage from embryogenesis (from oviposition to the cellular blastoderm), an increase in the consumption of glycogen occurs, while the second stage (from the blastoderm to larval hatching) is characterized by intense gluconeogenesis (Braz et al, 2019). Hexokinase (EC 2.7.1.1), the first enzyme of glycolytic pathways, phosphorylates glucose to glucose-6-phosphate, the essential substrate to cellular biosynthesis (Moraes et al, 2007).…”

Section: Enzymes Related To Energetic Metabolismmentioning

confidence: 99%

“…The infestation by this tick leads to weight loss, reduced milk production, decreased leather quality and higher production costs. Also, it is one of the vectors of potentially fatal tick-borne diseases, such as anaplasmosis and babesiosis (Lew-Tabor and Rodriguez Valle, 2016; Braz et al ., 2019).…”

Section: Introductionmentioning

confidence: 99%

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Molecular targets for the development of new acaricides againstRhipicephalus microplus: a review

et al. 2022

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The cattle tick Rhipicephalus microplus is an ectoparasite with high economic importance to bovine culture, mainly in tropical and subtropical regions. The resistance of the tick from the commercial acaricides has hindered its control, thus motivating the search for new strategies. The purpose of this study was to perform a critical review about the main molecular targets of R. microplus that are useful for the discovery of new acaricides. Bibliographic search was conducted in the databases PubMed, ScienceDirect and CAB Direct, using the following descriptors: ‘Rhipicephalus microplus’, ‘Boophilus microplus’, ‘molecular targets’ and ‘action’, published between 2010 and 2021. Out of the 212 publications identified, 17 articles were selected for study inclusion. This review described 14 molecular targets and among these 4 are targets from commercial acaricides. Most of them are enzymes to catalyse important reactions to tick survival, related to energetic metabolism, mechanisms of biotransformation and neurotransmission. The data will be helpful in the development of new more effective and selective acaricides.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Enzymes Related To Energetic Metabolismmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Molecular targets for the development of new acaricides againstRhipicephalus microplus: a review

et al. 2022

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Molecular characterization of hexokinase (HK) in Haemaphysalis longicornis and evaluation of HK protein‐ and DNA‐based vaccines against adult ticks

Wang

et al. 2023

Pest Management Science

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Background Haemaphysalis longicornis is an obligate hematophagous ectoparasite, which transmits various pathogens to humans, livestock and wild animals. Hexokinase (HK) is a key regulatory enzyme of the glycolytic pathway in the organisms. However, little is known about hexokinase and its functions in ticks. Results The open reading frame of the H. longicornis HK (HlHK) gene was 1425 bp and encoded a protein of 474 amino acids, containing conserved domains for glucose, glucose 6‐phosphate, and adenosine triphosphate. The expression of HlHK gene was detected at different developmental stages and in different tissues of unfed female ticks. Enzyme‐linked immunosorbent assay revealed that both HK protein‐ and DNA‐based vaccines increased the antibody levels of the immunized animals. A vaccination trail on rabbits against H. longicornis infestation indicated that the rHlHK protein and HlHK DNA vaccines reduced the number of attached female ticks by 9% and 12%, egg mass weight by 36% and 34%, and egg hatching rate by 41% and 17%, respectively. Overall, protein vaccination conferred 65.6% protection against adult female ticks, whereas the DNA vaccine conferred 51.8% protection. Conclusion This is the first report of the molecular characterization of the HK protein and sequencing of the HK gene from H. longicornis. Positive results from vaccination trials on rabbits of the recombinant HK protein and HK DNA suggest that these novel anti‐tick vaccines potentially can be used as viable tick control tools for the management of the Asian longhorned tick. Additionally, inhibition of glucose metabolism may be a new strategy for tick control. © 2023 Society of Chemical Industry.

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Anonaine from Annona crassiflora inhibits glutathione S-transferase and improves cypermethrin activity on Rhipicephalus (Boophilus) microplus (Canestrini, 1887)

Bezerra¹,

Tavares²,

Rocha³

et al. 2022

Experimental Parasitology

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Inhibition of energy metabolism by 3-bromopyruvate in the hard tick Rhipicephalus microplus

Cited by 3 publications

References 39 publications

Molecular targets for the development of new acaricides againstRhipicephalus microplus: a review

Molecular targets for the development of new acaricides againstRhipicephalus microplus: a review

Molecular characterization of hexokinase (HK) in Haemaphysalis longicornis and evaluation of HK protein‐ and DNA‐based vaccines against adult ticks

Anonaine from Annona crassiflora inhibits glutathione S-transferase and improves cypermethrin activity on Rhipicephalus (Boophilus) microplus (Canestrini, 1887)

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