Inhibition of DNA synthesis and chromosome aberrations in cultured Ehrlich ascites tumor cells following treatment with luteoskyrin

Schachtschabel, D.O.; Zilliken, F.; Saitō, Mamoru; Foley, G. E.

doi:10.1016/0014-4827(69)90362-0

Cited by 24 publications

(5 citation statements)

References 18 publications

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“…Experimente an Ascites-Zellen in Monolayer-Kulturen wurden nach der in 1. c. 19 beschriebenen Methodik durchgeführt. Zur Methode der Therapieversuche siehe 1. c. 18 .…”

Section: Methodikunclassified

Synthese und biochemische Eigenschaften von heterocyclischen Thioäthern des 2-Chlor-6-mercaptopurins / Synthesis and Biochemical Properties of Heterocyclic Thioethers of 2-Chloro-6-mercaptopurine

Niebch

Schneider

1972

Zeitschrift Für Naturforschung B

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The synthesis of the following thioethers of 2-chloro-6-mercaptopurine is described: 2-chloro-6-[4-phenyl-imidazole-2-thio] purine; 2-chloro-6-[4-guanidopropyl-imidazole-2-thio] purine; 2-chloro-6-[4-t.butyl-thiazole-2-thio] purine;2-chloro-6-[4-phenyl-thiazole-2-thio] purine; 2-chloro-6-[4-methylthiazole-2-thio] purine;2-chloro-6-[4-carbethoxymethyl-thiazole-2-thio] purine; 2-chloro-6-[4-carboxamido-5-amino-thiazole-2-thio] purine; 2-chloro-6-[5-phenyl-thiadiazole (1,3,4) -2-thio] purine; 2-chloro-6-[5-t.butyl-thiadiazole (1,3,4) -2-thio] purine; 2-chloro-6-[tetrazole-5-thio] purine. The thioether bond of these compounds is cleaved by nucleophilic agents especially by mercaptanes under mild conditions. The compounds are not metabolized by ascites cells and preferentially bound to the cell surface. They are inhibitors of DNA-, RNA-, and proteinsynthesis and of proliferation of Ascites cells in a different degree; the ascites carcinoma of mice was not influenced. A possible mechanism of action of their metabolic effects is discussed.

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“…Experimente an Ascites-Zellen in Monolayer-Kulturen wurden nach der in 1. c. 19 beschriebenen Methodik durchgeführt. Zur Methode der Therapieversuche siehe 1. c. 18 .…”

Section: Methodikunclassified

Synthese und biochemische Eigenschaften von heterocyclischen Thioäthern des 2-Chlor-6-mercaptopurins / Synthesis and Biochemical Properties of Heterocyclic Thioethers of 2-Chloro-6-mercaptopurine

Niebch

Schneider

1972

Zeitschrift Für Naturforschung B

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“…The origin and maintenance of the Ehrlich ascites tumor cell line has been described elsewhere [14,15,17], These cells were cultured in Eagle's basal medium (Gibco) containing 0.1 mg streptomycin (Farbwerke Hoechst) and 100IU penicillin-G-sodium (Griinenthal) per milliliter and supplemented with 10%> fetal calf serum (Gibco).…”

Section: Methodsmentioning

confidence: 99%

Effect of Pulverized Implantation Materials (Plastic and Glass Ceramic) on Growth and Metabolism of Mammalian Cell Cultures

Schachtschabel¹,

Blencke²

1976

Eur Surg Res

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The effect of pulverized plastic and glass-ceramic materials (methylmetacrylate, MMA), which are used as implantation materials in surgical medicine, on cell growth, DNA synthesis rate (adjudged by incorporation of ³H-thymidine into DNA), glucose consumption and lactate production (glycolytic rate) was studied in asynchronous monolayer cultures of rather fast proliferating Ehrlich ascites tumor cells and rather slowly proliferating diploid human fibroblasts. Exposure of Ehrlich ascites cells to high concentrations (2 mg/ml; 10 ml medium per culture) of ceramic or plastic material resulted in a gradual inhibition of cell growth and DNA synthesis rate. Protein synthesis, as measured by the incorporation of ³H-leucine, was somewhat less affected than DNA synthesis. Also, the glycolytic rate of Ehrlich ascites cells was slightly but significantly decreased in the presence of 2 mg/ml ceramics or MMA. Exposure of Ehrlich ascites cells to 0.2 or 0.02 mg/ml over a period of 46 h revealed none or only slight inhibitory effects on growth, DNA synthesis or glycolytic rate. On the other side, growth, DNA synthesis and glycolytic rates of human fibroblasts were nearly not affected by the presence of the same concentrations (up to 2 mg/ml, incubation period: 92 h) of pulverized ceramic or plastic material (MMA). It is suggested that this differential cellular sensitivity might be related to differences in the binding (to the cell surface) or uptake of these substances and possibly to differential intracellular lysosomal activation.

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“…Luteoskyrin is also toxic to Chang's liver cells, to HeLa cells, and to protozoa such as Paramecium caudatum (Kawamoto et al, 1964) and Tetrahymena pyriformis (Ueno and Saheki, 1968; Mouton and Fromageot, 1971). This pigment induces chromosome aberrations in cultures of Ehrlich ascites (Schachtschabel et al, 1969), and chromosome breakage in cultured human leukocytes (Keutel and Mockel, 1969). Moreover, Ls and Rg have been proved to inhibit both in vitro and in vivo, essential biological functions such as replication, transcription, and DNA repair (Umeda et al, 1963;Umeda, 1964;Schachtschabel et al, 1969; Mouton and Fromageot, 1971;Sentenac et al, 1967; Ruet et al, 1973).…”

mentioning

confidence: 99%

“…This pigment induces chromosome aberrations in cultures of Ehrlich ascites (Schachtschabel et al, 1969), and chromosome breakage in cultured human leukocytes (Keutel and Mockel, 1969). Moreover, Ls and Rg have been proved to inhibit both in vitro and in vivo, essential biological functions such as replication, transcription, and DNA repair (Umeda et al, 1963;Umeda, 1964;Schachtschabel et al, 1969; Mouton and Fromageot, 1971;Sentenac et al, 1967; Ruet et al, 1973). In relation to these inhibitory effects, both pigments have been found to form, respectively, two types of complexes with DNA (or synthetic polynucleotides) in the presence of divalent cations.…”

mentioning

confidence: 99%

Isolation and characterization of luteoskyrin and rugulosin, two hepatotoxic anthraquinonoids from Penicillium islandicum Sopp. and Penicillium rugulosum Thom.

Jc¹,

Pp²,

Toma³

et al. 1976

J. Agric. Food Chem.

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Inhibition of DNA synthesis and chromosome aberrations in cultured Ehrlich ascites tumor cells following treatment with luteoskyrin

Cited by 24 publications

References 18 publications

Synthese und biochemische Eigenschaften von heterocyclischen Thioäthern des 2-Chlor-6-mercaptopurins / Synthesis and Biochemical Properties of Heterocyclic Thioethers of 2-Chloro-6-mercaptopurine

Synthese und biochemische Eigenschaften von heterocyclischen Thioäthern des 2-Chlor-6-mercaptopurins / Synthesis and Biochemical Properties of Heterocyclic Thioethers of 2-Chloro-6-mercaptopurine

Effect of Pulverized Implantation Materials (Plastic and Glass Ceramic) on Growth and Metabolism of Mammalian Cell Cultures

Isolation and characterization of luteoskyrin and rugulosin, two hepatotoxic anthraquinonoids from Penicillium islandicum Sopp. and Penicillium rugulosum Thom.

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