Effect of Pulverized Implantation Materials (Plastic and Glass Ceramic) on Growth and Metabolism of Mammalian Cell Cultures

Schachtschabel, D.O.; Blencke, B.-A.

doi:10.1159/000127849

Cited by 11 publications

(3 citation statements)

References 12 publications

(14 reference statements)

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“…Indeed, tritiated thymidine uptake and incorporation is commonly used in the field of tissue engineering for quantitation of cellular proliferation on biomaterial scaffolds or substrates 6–9. Similarly, cellular protein synthesis has been quantitated by using radiolabeled amino acids in cultures grown on biomaterial substrates 9–13…”

Section: Introductionmentioning

confidence: 99%

Potential pitfalls of radiolabel adsorption to ceramic biomaterials

Parker

Upton

Vellinga

et al. 2005

J Biomedical Materials Res

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The use of radiolabeled precursor molecules for the metabolic analysis of cell functions is commonplace. Tritiated thymidine, in particular, has been used to quantitate cellular proliferation in numerous cells, including osteoblasts, when cultured on various biomaterials. Our aim was to assess cellular protein synthesis and proliferation, on a range of fluoride ion-substituted hydroxyapatites. Initially, we used a classical metabolic analysis strategy with radiolabeled tracer molecules. Our results suggested that these materials supported enhanced protein synthesis and proliferation of SaOS-2 human osteoblast-like cells. However, control samples also revealed enhanced adsorption of the radiolabeled tracer. We have shown that this arises because partially fluoride ion-substituted hydroxyapatite exhibits enhanced adsorptive characteristics of radiolabeled leucine and thymidine over tissue culture plastic, hydroxyapatite, and fluoroapatite. Moreover, manual cell count data obtained through SEM analysis showed no significant difference in cell proliferation between any of the materials, further indicating that our initial results were artifacts. These results highlight the use and reporting of appropriate cell-free controls are critical in bioassays examining functional responses of cells to biomaterials, and if absent, may confound accurate data interpretation. Our findings have general implications for investigations of cell function on other novel ceramic biomaterials.

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Section: Introductionmentioning

confidence: 99%

Potential pitfalls of radiolabel adsorption to ceramic biomaterials

Parker

Upton

Vellinga

et al. 2005

J Biomedical Materials Res

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“…Die ersten vorgestellten Zellkultursysteme zum Biomaterialien-Screening benutzten wenig differenzierte, embryonale oder neoplastische Zellinien, die allerdings in situ nicht in Kontakt mit dem implantierten Material stehen [10,14]. Diese erwiesen sich zudem als wenig sensitiv.…”

Section: Introductionunclassified

Biokompatibilitätsprüfung von Implantatmaterialien mit humanen Knochenmarkszellkulturen - Biocompatibility-testing of Implant Materials by Human Bone Marrow Gell Cultures

Wilke¹,

Schröder²,

Orth³

et al. 1993

Biomedizinische Technik/Biomedical Engineering

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A cell culture biocompatibility test for implant materials is described. For the first time, the human bone marrow cells that actually come into direct contact with the implanted biomaterial can be employed. The usefulness of this method is shown for materials which have long been used in the clinical situation (hydroxyapatite, commercially pure titanium and ultra-high molecular weight polyethylene). After two weeks in culture, light- and fluorescence-microscopic and SEM studies revealed significant differences in cell growth. Maximum compatibility was shown by the hydroxyapatite specimen. In the case of commercially pure titanium we found somewhat decreased biocompatibility. Polyethylene was associated with distinct signs of cytotoxicity. These results are compatible with those of animal experiments and clinical experience. Our system would thus appear to be a useful screening test for some implantation materials, and may help reduce the need for animal tests.

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“…Hydrogels are attractive as biomaterials; they are highly permeable to water, ions, and small molecules (1). Hydrogels such as poly(2-hydroxyethylmethacrylate) (HEMA) and other synthetic polymers are relatively nontoxic and well tolerated when implanted in vivo (2,3) or when added to an actively metabolizing tissue-culture system (4)(5)(6).…”

mentioning

confidence: 99%

Use of collagen-hydroxyethylmethacrylate hydrogels for cell growth.

Civerchia-Perez¹,

Faris²,

Lapointe³

et al. 1980

Proc. Natl. Acad. Sci. U.S.A.

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Collagen-hydroxyethylmethacrylate hydrogels were prepared by polymerizing monomeric hydroxyethylmethacrylate in the presence of various concentrations of soluble native collagen. The resulting transparent hydrogels were evaluated as substrata for growth of IMR-90 human embryonic lung fibroblasts. Without collagen no significant growth occurred, whereas a dose-response curve expressing maximal cell growth against collagen concentration could be constructed quite readily by the use of appropriate hydrogels. The method allows for quantification of the collagen contribution to cell growth and, in a more general sense, provides the foundation for a relatively easy procedure to probe mechanisms of cell adhesion and cell differentiation. Among the many synthetic polymers presently used as biomaterials are those known as hydrogels. The term "hydrogel" refers to a broad class of polymeric materials that are swollen extensively in water but that do not dissolve in water (1). They have been used in a wide variety of biomedical applications and may be synthesized from monomers or monomers mixed with polymers. Hydrogels are attractive as biomaterials; they are highly permeable to water, ions, and small molecules (1). Hydrogels such as poly(2-hydroxyethylmethacrylate) (HEMA) and other synthetic polymers are relatively nontoxic and well tolerated when implanted in vivo (2, 3) or when added to an actively metabolizing tissue-culture system (4-6).The implantation of a biological material such as collagen results in good tissue tolerance both in vivo (7-15) and in vitro (16)(17)(18)(19)(20)(21). Collagen has been used in many biomedical applications such as in the dialysis membrane of an artificial kidney (7,(22)(23)(24), an artificial corneal membrane (8, 9), as vitreous body (10-12), in skin and blood vessels (13,14), and as a surgical hemostatic agent (15). In 1969, Chvapil et al. .(25) described good tissue tolerance in vivo of a collagen-hydrophilic polymer that they had constructed from a calf-hide collagen sponge immersed in HEMA monomer, allowing polymerization to occur on the sponge surface. They attributed the biocompatibility of this material to the porosity of the sponge, which permitted the ingrowth of blood vessels. In 1977, Shimizu et al. (26) reported high tissue compatibility of laminar copolymers of bovine collagen and various synthetic polymers that they had constructed by applying plasma discharge and y-irradiation to effect crosslinkage between a layer of collagen coated onto a layer of synthetic polymer. More recent studies involving collagen-cell surface interactions have focused on the role of fibronectin (27,28).In a study by Folkman and Moscona (29), poly(HEMA) coating was used as a means of preventing fibroblast growth on standard tissue-culture flasks (Falconware). Similarly, a morphologic study of mouse fibroblasts grown on syntheticcoated coverslips found poly(methylmethacrylate) and other polymers to be unfavorable surfaces for cell adhesion and growth (30). In the present communication, w...

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Effect of Pulverized Implantation Materials (Plastic and Glass Ceramic) on Growth and Metabolism of Mammalian Cell Cultures

Cited by 11 publications

References 12 publications

Potential pitfalls of radiolabel adsorption to ceramic biomaterials

Potential pitfalls of radiolabel adsorption to ceramic biomaterials

Biokompatibilitätsprüfung von Implantatmaterialien mit humanen Knochenmarkszellkulturen - Biocompatibility-testing of Implant Materials by Human Bone Marrow Gell Cultures

Use of collagen-hydroxyethylmethacrylate hydrogels for cell growth.

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