Inhibition of beef liver hydrolytic enzymes by organophosphorus pesticides.  Quantal inhibition characteristics of several organophosphorus compounds, using o-nitrophenyl butyrate as substrate

Villeneuve, D. C.; McKinley, W. P.

doi:10.1021/jf60156a030

Cited by 12 publications

(4 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Essentially the same procedure was used as described previously (Villeneuve and McKinley, 1968). One milliliter of the diluted organic solvent (either with or without the pesticide) was pipeted into a 15-ml.…”

Section: Procedure;mentioning

confidence: 99%

“…of final solution) as well as the solvent, while a control tube without solvent or pesticide was subjected to the same procedure. At the end of 15 minutes, color development was carried out as described previously (Villeneuve and McKinley, 1968), and the absorbance of each tube read against a reagent blank. The per cent of activation and inhibition were calculated from the following equations: --' X 100 absorbance (solvent)…”

Section: Procedure;mentioning

confidence: 99%

“…In an earlier paper (Villeneuve and McKinley, 1968), it was observed that ethanol not only enhanced carboxylesterase activity, but also decreased the inhibitory effect of the pesticide Trithion. The purpose of this study was to determine the effects of other organic solvents on enzyme activation and inhibition and to select the most suitable organic solvent for further inhibition studies.…”

mentioning

confidence: 93%

“…The solutions, substrate, and inhibitor used in this study have been described previously (Villeneuve and McKinley, 1968).…”

mentioning

confidence: 99%

See 3 more Smart Citations

Inhibition of Beef Liver Hydrolytic Enzymes by Organophosphorus Pesticides

Villeneuve¹,

Mulkins²,

Trenholm³

et al. 1969

J. Agric. Food Chem.

View full text Add to dashboard Cite

The effect of several organic solvents on the rate at which the carboxylesterases of beef liver hydrolyze u-nitrophenylbutyrate is reported. The organic solvents studied were acetone, methanol, ethanol, «propanol, 2-propanol, glycerol, dimethyl sulfoxide (DMSO), «-butanol, rec-butanol, rcrt-butanol, isobutanol, and «-pentanol. These solvents also affected the inhibition of liver enzymes by the pesticide Ruelene. A preliminary study of these effects was carried out at the 2.5% solvent concentration. This was followed by a more detailed study on acetone, methanol, glycerol, and DMSO at concentrations of up to 20%. The organic solvents were classified according to their effects on enzyme activation and inhibition: those which activated and decreased inhibition, those which deactivated and decreased inhibition, those which activated and increased inhibition, and those which deactivated and increased inhibition. The concentration of the solvent was a critical factor in enzyme activation and inhibition. The effects of these solvents on the optical properties of the reaction product, -nitrophenol, were also studied.

show abstract

Section: Procedure;mentioning

confidence: 99%

Section: Procedure;mentioning

confidence: 99%

mentioning

confidence: 93%

“…The solutions, substrate, and inhibitor used in this study have been described previously (Villeneuve and McKinley, 1968).…”

mentioning

confidence: 99%

See 2 more Smart Citations