Abstract:The aggregation of proteins has been associated with several aspects of daily life, including food processing, blood coagulation and many neurodegenerative infections.
“…To gain more insight, atomic force microscopy was also employed; it has been proved to be a powerful tool in the study of fibril formation. 27 AFM analysis was performed to visualize the extent of disruption of β-lg samples aggregated alone or with coumarin derivatives at molar concentration ratios of 1 : 1.…”
Aggregation of β-lactoglobulin (β-lg) was inhibited through the stabilization of the native structure by various non-covalent interactions of coumarin derivatives. The 8-hydroxy compound was most effective against the self-assembly of β-lg.
“…To gain more insight, atomic force microscopy was also employed; it has been proved to be a powerful tool in the study of fibril formation. 27 AFM analysis was performed to visualize the extent of disruption of β-lg samples aggregated alone or with coumarin derivatives at molar concentration ratios of 1 : 1.…”
Aggregation of β-lactoglobulin (β-lg) was inhibited through the stabilization of the native structure by various non-covalent interactions of coumarin derivatives. The 8-hydroxy compound was most effective against the self-assembly of β-lg.
“…Docking study is a tool, generally used to predict the position and binding interactions of molecule into proteins or DNAs. 67,68 Among the four methionine amino acids of β-lg, Met7 is located at the surface of the protein and easy to oxidize, Met24 and Met107 are located inside the hydrophobic calyx of β-lg and Met145 is also in the hydrophobic core ( Fig. 6 ).…”
“…ThT is a cationic dye having a benzothiazole nucleus that binds strongly with the crossed b-sheet structure of the amyloid fibrils and causes a remarkable enhancement of the emission maxima. 46,47 ThT stock solution of 3.136 mM (1 mg mL À1 ) was prepared by dissolving ThT in HPLC water. From each set of heat incubated and control solutions, aliquots were withdrawn and mixed with ThT solution.…”
Section: Thioflavin T (Tht) Fluorescence Measurementsmentioning
Metal ions of the later part of the first-row transition series (Fe, Co, Ni, Cu, and Zn) can form bonds through the carboxylate, hydroxyl, thiol, and imidazole side chains of...
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