Coumarin derivatives inhibit the aggregation of β-lactoglobulin

Abstract

Aggregation of β-lactoglobulin (β-lg) was inhibited through the stabilization of the native structure by various non-covalent interactions of coumarin derivatives. The 8-hydroxy compound was most effective against the self-assembly of β-lg.

Analysis of the Binding Mechanism of Bioactive Coumarins with Ovalbumin: Further Investigation into the Inhibitory Effects toward Protein Fibrillation

“…However, the absorption intensity becomes less than that of heat-treated OVA (HTO) in the presence of 7-HC and 4-Me-7-HC, indicating a reduced level of aggregation formation, which ultimately suggests that the coumarin derivatives act as a good antifibrillating agent. Thioflavin T (ThT), a different amyloid binding dye, when binds to the hydrophobic site of amyloid aggregates displayed an increased fluorescence emission intensity . So, the intensity of the ThT emission spectrum can be used to determine the aggregate formation and the effectiveness of the antifibrillating agents (7-HC and 4-Me-7-HC).…”

“…Thioflavin T (ThT), a different amyloid binding dye, when binds to the hydrophobic site of amyloid aggregates displayed an increased fluorescence emission intensity. 52 So, the intensity of the ThT emission spectrum can be used to determine the aggregate formation and the effectiveness of the antifibrillating agents (7-HC and 4-Me-7-HC). Here, the ThT emission intensities from each of the heat-treated OVA (HTO) without and with the coumarin derivatives, i.e., HTO, HTO-7-HC, and HTO-4-Me-7-HC, were distinct and correlated with the level of aggregation formation in the various protein solutions.…”

In vitro retardation and modulation of human insulin amyloid fibrillation by Fe3+ and Cu2+ ions

“…The percent change in fluorescence intensity (Fp) was calculated 48 using eqn (1): F P = {( F t − F 0 )/ F 0 } × 100where F t is the fluorescence at a specific time point and F 0 is the fluorescence at the starting time of incubation.…”

“…where F t is the fluorescence at a specific time point and F 0 is the fluorescence at the starting time of incubation. Percent inhibition was also calculated 48 using eqn (2):…”

“…The effect of metal ions on the aggregation of heat exposed insulin was monitored by RLS measurement 48 by observing the emission at 307 nm after exciting the solution at 307 nm using a Horiba Scientific Spectrofluorometer (Model: FLUOROMAX-4C, Serial no. 1734D-4018-FM and Fluoromax Software).…”