Inhibition of Alpha-glucosidase and Amylase by Luteolin, a Flavonoid

Kim, Jong-Sang; Kwon, Chong-Suk; Son, Kun Ho

doi:10.1271/bbb.64.2458

Cited by 545 publications

(359 citation statements)

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“…These enzymes facilitate hydrolysis of starch to glucose with consequential increase in the systemic concentration of glucose. This increased hyperglycemia may constitute an independent risk factor for the development of microvascular and macrovascular complications of diabetes mellitus which is mediated through oxidative stress (Kim et al, 2000). Hence, the inhibition of these enzymes activities delays glucose absorption and moderates postprandial blood sugar level (Sabiu and Ashafa, 2016).…”

Section: Discussionmentioning

confidence: 99%

KINETICS OF MODULATORY ROLE OF Cyperus esculentus L. ON THE SPECIFIC ACTIVITY OF KEY CARBOHYDRATE METABOLIZING ENZYMES

Sabiu

Ajani

Sunmonu

et al. 2017

Afr J Tradit Complement Altern Med

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Background: The continuous search for new lead compounds as viable inhibitors of specific enzymes linked to carbohydrate metabolism has intensified. Cyperus esculentus L. is one of the therapeutically implicated botanicals against several degenerative diseases including diabetes mellitus. Materials and Methods: This study evaluated the antioxidant and mechanism(s) of inhibitory potential of aqueous extract of C. esculentus on α-amylase and α-glucosidase in vitro. The extract was investigated for its radical scavenging and hypoglycaemic potentials using standard experimental procedures. Lineweaver-Burke plot was used to predict the manner in which the enzymes were inhibited. Results: The data obtained revealed that the extract moderately and potently inhibited the specific activities of α-amylase and α-glucosidase, respectively. The inhibition was concentration-related with respective IC50 values of 5.19 and 0.78 mg/mL relative to that of the control (3.72 and 3.55 mg/mL). The extract also significantly scavenged free radicals and the effects elicited could be ascribed to its phytoconstituents. Conclusion:The respective competitive and non-competitive mode of action of the extract is due to its inhibitory potentials on the activities of α-amylase and α-glucosidase. Going forward, in addition to completely characterize the exact compound(s) responsible for the elicited activity in this study, pertinent attention will be given to the in vivo evaluation of the identified constituents.

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Section: Discussionmentioning

confidence: 99%

KINETICS OF MODULATORY ROLE OF Cyperus esculentus L. ON THE SPECIFIC ACTIVITY OF KEY CARBOHYDRATE METABOLIZING ENZYMES

Sabiu

Ajani

Sunmonu

et al. 2017

Afr J Tradit Complement Altern Med

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“…However, these fruits may have different inhibitory effects on α-amylase and α-glucosidase of different origins. Reportedly, various natural products, such as flavone, and flavonoids, inhibit α-amylase and α-glucosidase activities (Havsteen, 1983;Kim et al, 2000). Moreover, polyphenols also have antihyperglycemic effects (Hossain et al, 2002;Hanamura et al, 2006), and inhibit the development of diabetes (Zunimo et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

“…Various flavonoids and non-flavonoids have been reported as showing radical scavenging activity (Sawa et al, 1999): flavone, and flavonoids inhibit α-amylase and α-glucosidase activities (Havsteen, 1983;Kim et al, 2000); polyphenols have anti-hyperglycemic effects (Hossain et al, 2002;Hanamura et al, 2006), and inhibit the development of diabetes (Zunimo et al, 2007). Since glucose release from food sources is the main factor affecting post-prandial hyperglycaemia, retardation of the digestion of starch by key gastrointestinal enzymes can be of benefit to diabetic patients (Puls and Keup, 1973).…”

Section: Introductionmentioning

confidence: 99%

Total Phenolic Content, Antioxidative, Anti-amylase, Anti-glucosidase, and Antihistamine Release Activities of Bangladeshi Fruits

Hossain

Tsujiyama

Takasugi

et al. 2008

FSTR

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To seek out a cheap source of dietary polyphenols and antioxidants along with anti-amylase and antiglucosidase activities, ethanol extracts of eleven cheap Bangladeshi fruits were investigated. The extracts were also examined for anti-allergic activity using rat peritoneal exudate cells exposed to the calcium ionophore A23187. Phyllanthus emblica (emblic myrobalan) had the highest total polyphenol content (339 mg gallic acid equivalent (GAE)/g), followed by Syzygium cumini (Indian blackberry; 192.3 mg GAE/g), and Aegle marmelos (wood apple; 53.7 mg GAE/g). P. emblica, and S. cumini also exhibited the most potent DPPH radical scavenging activity, with an IC 50 of 2.1 and 8.6 μg/mL respectively. These extracts also showed promising reducing powers with P. emblica having the greatest such activity (optical density (O.D.) 1.66), followed by S. cumini (O.D. 1.34), at a concentration of 0.2 mg/mL. The extracts of Artocarpus heterophyllus (jackfruit) and S. cumini showed promising chelating activities. At a concentration of 1 mg/ mL, Dillenia indica (chalta) showed the highest inhibition of α-amylase activity (60%), and A. marmelos, D. indica, P. emblica, Spondias dulcis (hog-plum) & S. cumini completely inhibited α-glucosidase activity (100%). Apart from A. heterophyllus, D. indica and Phyllanthus acidus (star-gooseberry), all other extracts inhibited the release of histamine from the peritoneal exudate cells, with S. cumini having the strongest effect. These fruits therefore have activities beneficial to physiological health. Keywords: antioxidant, anti-amylase, anti-glucosidase, anti-allergy, antihistamine release, fruits, polyphenol *To whom correspondence should be addressed. Email: aoshima@yamaguchi-u.ac.jp IntroductionFruits are major sources of health promoting agents. Of these agents, antioxidants are most important because they inhibit the initiation of lipid peroxidation, which is related to aging and the pathogenesis of diseases such as cardiovascular disorders, cancer, inflammation, and brain dysfunction (Ames, 1983;Shon et al., 2004). Various epidemiological studies have suggested that consumption of fruit and vegetables is associated with reduced risk of cardiovascular diseases and cancers (Kris-Etherton et al., 2002), neurodegenerative diseases such as Parkinson's and Alzheimer's diseases (Di Matteo and Esposito, 2003), as well as with inflammation and aging (Ames et al., 1993).Natural products are reportedly beneficial to physiological health. Various flavonoids and non-flavonoids have been reported as showing radical scavenging activity (Sawa et al., 1999): flavone, and flavonoids inhibit α-amylase and α-glucosidase activities (Havsteen, 1983;Kim et al., 2000); polyphenols have anti-hyperglycemic effects (Hossain et al., 2002;Hanamura et al., 2006), and inhibit the development of diabetes (Zunimo et al., 2007). Since glucose release from food sources is the main factor affecting post-prandial hyperglycaemia, retardation of the digestion of starch by key gastrointestinal enzymes can be of benefit to...

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“…This is appropriate with the substrate of α-glucosidase, which is a food starch and carbohydrate (glycogen) [33]. Alpha-glucosidase inhibitors focusing on reducing the digestion of carbohydrate are the most common and efficacious agents utilized for the treatment of Type 2 diabetes [34,35]. Since α-glucosidase inhibitors prevent the hybridization of carbohydrates into glucose, a lot of carbohydrates remain in the intestine.…”

Section: Discussionmentioning

confidence: 99%

Antidiabetic Potential of the Oyster Mushroom Pleurotus Florida (Mont.) Singer

Prabu

Kumuthakalavalli

2017

Int J Curr Pharm Sci

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Objective: The present investigation comprises, in vitro antidiabetic activity such as α-amylase and α-glucosidase inhibitory activities and in vivo antidiabetic activity of methanolic extract of Pleurotus florida. Methods:The fruiting bodies of Pleurotus florida were obtained from Mushroom Unit, Department of Biology, Gandhigram Rural Institute-Deemed University, Gandhigram, Dindigul, Tamil Nadu, India. Sample preparation, qualitative phytochemical analysis, in vitro antidiabetic activities namely α-amylase and α-glucosidase inhibitory activity and in vivo antidiabetic activity namely evaluation of alloxan induced diabetic rats were carried out following the methods reported previously. Results:In vitro and in vivo antidiabetic activity of P. florida exhibited significant results for its α-amylase (94.93±1.75 % at 1000 µg/ml) and α-glucosidase inhibitory activity (84.90±0.42 % at 1000 µg/ml) in a dose-dependent manner. The extract also showed significant antidiabetic activity on in vivo (p<0.05) at the tested dose level (200 mg/kg b. w) this was comparable to Glibenclamide, a standard antidiabetic drug. Conclusion:The presence of phytochemicals namely phenols, flavonoids, saponins, tannins and terpenoids may be responsible for such antidiabetic activity. These results reveal that P. florida can be used as a potential antidiabetic agent.

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Inhibition of Alpha-glucosidase and Amylase by Luteolin, a Flavonoid

Cited by 545 publications

References 1 publication

KINETICS OF MODULATORY ROLE OF Cyperus esculentus L. ON THE SPECIFIC ACTIVITY OF KEY CARBOHYDRATE METABOLIZING ENZYMES

KINETICS OF MODULATORY ROLE OF Cyperus esculentus L. ON THE SPECIFIC ACTIVITY OF KEY CARBOHYDRATE METABOLIZING ENZYMES

Total Phenolic Content, Antioxidative, Anti-amylase, Anti-glucosidase, and Antihistamine Release Activities of Bangladeshi Fruits

Antidiabetic Potential of the Oyster Mushroom Pleurotus Florida (Mont.) Singer

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