KINETICS OF MODULATORY ROLE OF Cyperus esculentus L. ON THE SPECIFIC ACTIVITY OF KEY CARBOHYDRATE METABOLIZING ENZYMES

Abstract

Background: The continuous search for new lead compounds as viable inhibitors of specific enzymes linked to carbohydrate metabolism has intensified. Cyperus esculentus L. is one of the therapeutically implicated botanicals against several degenerative diseases including diabetes mellitus. Materials and Methods: This study evaluated the antioxidant and mechanism(s) of inhibitory potential of aqueous extract of C. esculentus on α-amylase and α-glucosidase in vitro. The extract was investigated for its radical scavenging and hypoglycaemic potentials using standard experimental procedures. Lineweaver-Burke plot was used to predict the manner in which the enzymes were inhibited. Results: The data obtained revealed that the extract moderately and potently inhibited the specific activities of α-amylase and α-glucosidase, respectively. The inhibition was concentration-related with respective IC50 values of 5.19 and 0.78 mg/mL relative to that of the control (3.72 and 3.55 mg/mL). The extract also significantly scavenged free radicals and the effects elicited could be ascribed to its phytoconstituents. Conclusion:The respective competitive and non-competitive mode of action of the extract is due to its inhibitory potentials on the activities of α-amylase and α-glucosidase. Going forward, in addition to completely characterize the exact compound(s) responsible for the elicited activity in this study, pertinent attention will be given to the in vivo evaluation of the identified constituents.

Nutritional, antioxidant, angiotensin-converting-enzyme and carbohydrate-hydrolyzing-enzyme inhibitory activities of underutilized leafy vegetable: African wild lettuce (Lactuca taraxacifolia Willd)

“…The wild lettuce leaf protein isolates sample exhibited higher α-amylase and α-glucosidase enzyme inhibitory activities when compared with other experimental samples (WLF, WLE & WLR) and Acarbose (a synthetic antidiabetic agent). The inhibitory activity of wild lettuce leafy vegetable on α-amylase and α-glucosidase enzymes observed in this study was comparable to or higher than which reported for Cyperus esculentus [73], Adiantum caudatumand, Celosis argentea [74] and Cissus cornifolia [75]. It is well established that enzyme inhibitory agents usually block the activities of both α-amylase and α-glucosidase enzymes in the small intestine, and thereby limits digestion of oligosaccharides and disaccharides to monosaccharides, necessary for gastrointestinal absorption [76].…”

Inhibitory effects of Launaea taraxacifolia and Strychnos spinosa leaves extract on an isolated digestive enzyme linked to type -2 -diabetes mellitus

“…The significant difference in these values depicts the fact that SSE could be better than LTE and acarbose during pharmacotherapy. The IC 50 values of launaea taraxacifolia and Strychnos spinosa leaves extract recorded in this study is lower than that of Cyperus esculentus (Sabiu et al, 2017), Adiantum caudatum and Celosis argentea (Telagari et al, 2015) but higher than what has been reported for Cissus cornifolia (Chipiti et al, 2017).…”

Nutritional, antioxidant, glycaemic index and Antihyperglycaemic properties of improved traditional plantain-based (Musa AAB) dough meal enriched with tigernut (Cyperus esculentus) and defatted soybean (Glycine max) flour for diabetic patients

“…The tuber serves as a potentially valuable food for both human and animal in Southern Europe and many parts of developing countries (Pascual et al., 2000). The tubers contain appreciable amount of essential phytonutrients and phytochemicals, which are effective in the treatment and prevention of many diseases like coronary heart diseases, obesity, diabetes, and gastrointestinal diseases (Sabiu et al., 2017).…”