Inhibition by β-caryophyllene of ethyl methanesulfonate-induced clastogenicity in cultured human lymphocytes

Sotto, Antonella Di; Mazzanti, Gabriela; Carbone, Fabio; Hrelia, Patrizia; Maffei, Francesca

doi:10.1016/j.mrgentox.2010.04.008

Cited by 82 publications

(62 citation statements)

References 48 publications

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“…Therefore, we have discussed its genotoxicity potential as compared with other sesquiterpenes. Similar to our finding it was suggested that beta-caryophyllene by itself did not produce any cytotoxic and genotoxic effect, as shown by the value of the nuclear division index (NDI) and the frequency of micronuclei (MN) in human lymphocytes cultures (Di Sotto et al 2010). Likewise, Anter et al (2011) reported that apigenin, bisabolol, and protocatechuic acid did not exhibit any genotoxic effect in DNA fragmentation assay.…”

Section: Discussionsupporting

confidence: 89%

Cytotoxicity and genotoxicity of zingiberene on different neuron cell lines in vitro

et al. 2014

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The main objective of this study is to investigate the cytotoxic, genotoxic and antioxidant properties of zingiberene (ZBN) in an in vitro rat brain cell culture study. The cytotoxic effect was determined against the rat neuron and N2a neuroblastoma (N2a-NB) cell lines using the 3,(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, while the antioxidant activity was assessed using the total antioxidant capacity (TAC) and total oxidative stress (TOS) assays. The effects on DNA damage were also evaluated in this study by the single cell gel electrophoresis assay. The results indicated that ZBN has an anti-proliferative activity suppressing the proliferation of N2a-NB cells at concentrations over 50 mg L -1 and neuron cells at concentrations over 150 mg L -1. In addition, ZBN treatments at higher doses (B50 mg L -1 ) led to increases of TOS levels in N2a-NB cell cultures. However 25 mg L -1 of ZBN treatment caused increases of TAC levels in cultured neuron and N2a-NB cell cultures while ZBN at doses of 10-400 mg L -1 did not increase the number of total damage score in both cell lines. This study clearly indicates that ZBN has a significant potential to be used as a natural anticancer agent in cultured N2a-NBs.

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Section: Discussionsupporting

confidence: 89%

Cytotoxicity and genotoxicity of zingiberene on different neuron cell lines in vitro

et al. 2014

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“…Al-Zubairi et al (2010) reported that zerumbon (a sesquiterpene phytochemical from a type of edible ginger) did not induce genotoxicity in cultured human peripheral blood lymphocytes. In addition, Di Sotto et al (2010) reported that betacaryophyllene exhibited antigenotoxic activity in human peripheral blood lymphocyte cells against the toxicity of ethyl methanesulfonate and colcemid. On the other hand, Gil da Costa et al (2012) found that ptaquiloside (a norsesquiterpene) was genotoxic in cultured human lymphocytes by using the sister chromatid exchange (SCE) test.…”

Section: Discussionmentioning

confidence: 99%

In vitro cytotoxic, genotoxic, and oxidative effects of acyclic sesquiterpene farnesene

Çelik¹,

Toğar²,

Türkez³

et al. 2014

Turk J Biol

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IntroductionEssential oils are complex volatile natural compounds formed by aromatic plants as secondary metabolites. They are an important source of potential therapeutic compounds (Sherry et al., 2013). Essential oil constituents from aromatic herbs and dietary plants include monoterpenes, sesquiterpenes, oxygenated monoterpenes, oxygenated sesquiterpenes, and phenolics, among others (Bhalla et al., 2013).Sesquiterpenes, which are one of the most common terpenes, are a class of natural products with a diverse range of attractive industrial properties (Wang et al., 2011;Scalcinati et al., 2012). They are compounds containing 3 isoprene units, which have 15 carbons and 24 hydrogens per molecule (C 15 H 24 ). There are more than 10,000 kinds of sesquiterpenes (Davis and Croteau, 2000). They have long been investigated for biological activities including anticarcinogenic (

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“…Previous studies have reported that the essential oil of O. vulgare has antioxidant capacity, which has been linked to components such as thymol, carvacrol, d-terpinene, and p-cymene (Halici et al, 2005;Odabasoglu et al, 2004;Russo et al, 2002). In addition, there are many reports showing that components of the aqueous oregano extract, such as ursolic acid and rosmarinic acid, exert potent antioxidant activities by scavenging free radicals (Di Sotto et al, 2010;Lambert et al, 2001).…”

Section: Cyclophosphamidementioning

confidence: 99%

Protective effects ofOriganum vulgareethanol extract against cyclophosphamide-induced liver toxicity in mice

Habibi

Shokrzadeh²,

Chabra³

et al. 2014

Pharmaceutical Biology

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Context: Despite its wide clinical use, cyclophosphamide (CP), an alkylating chemotherapeutic agent, possesses many adverse effects, including hepatotoxicity. Because Origanum vulgare L. (Lamiaceae) has antioxidative properties, it might protect against above-mentioned damage.Objective: This study evaluated the protective effects of O. vulgare extract on CP-induced liver toxicity. Materials and methods: Mice were pretreated with aerial parts of O. vulgare ethanolic extract (intraperitoneally) at doses of 50, 100, 200, and 400 mg/kg for 7 consecutive days before the administration of a single 200 mg/kg intraperitoneal dose of CP 1 h after the last injection of O. vulgare. After 24 h, animals were anesthetized, blood samples and hepatic tissues were collected and used for biochemical and histological examination. Results: Serum levels of hepatic markers were increased after CP treatment but restored in the O. vulgare-pretreated groups. The serum ALT, AST, and ALP of the CP group were 196.49 ± 3.82, 143.78 ± 4.79, and 203.18 ± 3.81 IU/l, respectively. However, pretreatment with 400 mg/kg O. vulgare significantly decreased the serum ALT, AST, and ALP to 52.49 ± 2.18, 44.78 ± 2.06, and 65.62 ± 1.73 IU/l, respectively (p50.001). Histological examinations also confirmed the protective effects of O. vulgare against CP-induced liver toxicity. Discussion and conclusion: Our results reveal that O. vulgare with high amount of flavonoids and phenolic compounds induces potent hepatoprotective mechanisms against CP. Therefore, O. vulgare might help defend the body against the side effects, particularly hepatic damages induced by chemotherapeutic agents.

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Inhibition by β-caryophyllene of ethyl methanesulfonate-induced clastogenicity in cultured human lymphocytes

Cited by 82 publications

References 48 publications

Cytotoxicity and genotoxicity of zingiberene on different neuron cell lines in vitro

Cytotoxicity and genotoxicity of zingiberene on different neuron cell lines in vitro

In vitro cytotoxic, genotoxic, and oxidative effects of acyclic sesquiterpene farnesene

Protective effects ofOriganum vulgareethanol extract against cyclophosphamide-induced liver toxicity in mice

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