Protective effects of<i>Origanum vulgare</i>ethanol extract against cyclophosphamide-induced liver toxicity in mice

Habibi, Emran; Shokrzadeh, Mohammad; Chabra, Aroona; Naghshvar, Farshad; Keshavarz-Maleki, Razieh; Ahmadi, Amirhossein

doi:10.3109/13880209.2014.908399

Cited by 55 publications

(25 citation statements)

References 31 publications

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“…Furthermore, no increase in serum concentration of ALT and AST may provide evidence to protect of liver against hepatocellular degeneration (Al-Jaff, 2011). The presented study confirmed previous results (Habibi, et al, 2014;Saleh, 2014;Zhu, et al, 2014) on the decreasing effect of phytoadditives on the activities of plasma ALT and AST in animals. Meanwhile, Abd ElGhang and Ismail (2013) who used oregano essential oil in broiler feed, observed increase in the activity of ALT and AST.…”

Section: Resultssupporting

confidence: 81%

Effect of a phytogenic additive on blood serum indicator levels and fatty acids profile in fattening turkeys meat

Gálik

Wilkanowska

Bíro

et al. 2015

JCEA

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The aim of the study was to determine the effect of a phytogenic additive on blood serum indicator levels and fatty acids profile of breast, leg muscles and liver in fattening turkeys. The experiment was realized in private turkey farm and in the Department of Animal Nutrition, Faculty of Agrobiology and Food Resources, Slovak University of Agriculture in Nitra. A total of 300 clinically healthy female turkeys (broad-breasted white turkey, hybrid XL) were used in the experiment. Female turkeys were randomly divided into two groups (150 pcs per each). In the control group, turkey were fed with standard complete feed mixtures for fattening, in the experimental group, standard diets from the beginning to 12th week were supplemented with the a blend of essential oils from origanum, anise and citrus fruits as well as a prebiotic rich fructooligosaccharides in dosage 1kg per 1000 kg of feed mixture. Fattening lasted 18 weeks. Blood serum was collected at the end of the experiment, during the slaughter of birds. Samples of breast and leg muscles, and liver for fatty acids composition evaluation were collected during birds dissection (10 samples per each group). After the 12 weeks of phytoadditive supplementation, a tendency of lower activity of serum alanine aminotransferase (53.963 vs. 3.499 U/L) and aspartate aminotransferase (6.238 vs. 1.012 U/L) in experimental group of turkeys was found (P<0.01). The same tendency was analyzed in content of phosphorus (P<0.05). Compare to the control group, in the experimental group have been detected significantly (P<0.01) lower concentration of capric acid in leg muscle, lauric and myristic acid in both evaluated muscles, palmitoleic acid and stearic acid in the liver. Additionally, the phytoadditive supplementation markedly (P< 0.01) increased content of following SFA: lauric, myristic and palmitic acids in the liver, pentadecanoic, heptadecanoic and stearic in both evaluated muscles. In the case of unsaturated fatty acids have been detected that the phytogenic feed additive in breast and leg muscles of birds markedly increase the content of cis-11,14-eicosadienoic acid (0.209 vs 0.276 and 0.242 vs 0.298 % of crude fat, respectively); compare to control group, leg muscle of individuals from experimental group have higher (P>0.01) content of cis-8,11,14-eicosadienoic and arachidonic acids. The phytoadditive supplementation significantly (P<0.01) decreased content of some unsaturated fatty acids in turkeys tissues, as well. In experimental group of turkey have been recorded lower level of elaidic and oleic acids in the breast muscle and cis-11,14-eicosadienoic and arachidonic acids in the liver, compare to birds from control group.Keywords: additives, aromatic plants, blood serum, fatty acids profile, turkey Abstrakt Cieľom práce bolo determinovanie vplyvu fytogénneho aditíva na ukazovatele krvného sera a profil mastných kyselín v prsnom, stehennom svale a pečeni výrkmových moriek. Experiment bol realizovaný na privátnej farme moriek a Katedre výživy zvierat, Fakulta agro...

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Section: Resultssupporting

confidence: 81%

Effect of a phytogenic additive on blood serum indicator levels and fatty acids profile in fattening turkeys meat

Gálik

Wilkanowska

Bíro

et al. 2015

JCEA

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“…Cyclophosphamide also undergoes an inactivation pathway (to form the metabolite DECP) mediated by CYP3A13 in mice (Figure a; Pass et al, ). Treatment with cyclophosphamide (i.p., 300 mg·kg −1 , n = 5) induced hepatotoxicity in wild‐type mice (Figure b) consistent with previous reports (Habibi et al, ; Sheweita, El‐Hosseiny, & Nashashibi, ). Of note, the severity of hepatotoxicity was dosing time dependent with high levels at ZT2/22 and low levels at ZT10/14 (Figure b).…”

Section: Resultssupporting

confidence: 90%

Role of the CLOCK protein in liver detoxification

Zhao

Deng

et al. 2019

British J Pharmacology

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Background and Purpose: Whether and how circadian clock proteins regulate drug detoxification are not known. Here, we have assessed the effects of CLOCK (a core circadian clock protein) on drug metabolism and detoxification. Experimental Approach: Regulation by CLOCK protein of drug-metabolizing enzymes was assessed using Clock knockout (Clock −/− ) mice and Hepa-1c1c7/AML-12 cells. The relative mRNA and protein levels were determined by qPCR and Western blotting respectively. Toxicity and pharmacokinetic experiments were performed with Clock −/− and wild-type mice after intraperitoneal injection of coumarin or cyclophosphamide. Transcriptional gene regulation was investigated using luciferase reporter, mobility shift, and chromatin immunoprecipitation (ChIP) assays. Key Results: Clock deletion disrupted hepatic diurnal expressions of a number of drug-metabolizing enzymes in mice. In particular, CYP2A4/5 expressions were markedly down-regulated, whereas CYP2B10 was up-regulated. Positive regulation of Cyp2a4/5 and negative regulation of Cyp2b10 by CLOCK were confirmed in Hepa-1c1c7 and AML-12 cells. Based on a combination of luciferase reporter, mobility shift, and ChIP assays, we found that CLOCK activated Cyp2a4/5 transcription via specific binding to E-box elements in promoter region and repressed Cyp2b10 transcription through REV-ERBα/β (two target genes of CLOCK and transcriptional repressors of Cyp2b10). Furthermore, Clock ablation sensitized mice to coumarin toxicity by down-regulating CYP2A4/5-mediated metabolism (a detoxification pathway) and to cyclophosphamide toxicity by up-regulating CYP2B10-mediated metabolism (generating the toxic metabolite 4-hydroxycyclophosphamide).Conclusion and Implications: CLOCK protein regulates metabolism by the cytochrome P450 family and drug detoxification. The findings improve our understanding of the crosstalk between circadian clock and drug detoxification.

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“…Furthermore, no increase in serum concentration of ALT and AST may provide evidence to protect of liver against hepatocellular degeneration (Al-Jaff, 2011). Presented experiment did not confirm results of other authors (Habibi et al, 2014;Saleh, 2014;Zhu et al, 2014). Shi et al (2014) who added phytosterols into feed mixture of layers reported some minor changes in clinical chemistry parameters, but these changes were small and considered to be of no toxicological significance.…”

Section: Blood Parameters Of Laying Henscontrasting

confidence: 50%

The effect of essential plant oils on mineral composition of egg mass and blood parameters of laying hens

Herkeľ¹,

Gálik²,

Bíro³

et al. 2016

JCEA

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The aim of study was to analyze the dietary effect of pumpkin and flaxseed oils on mineral composition of egg mass and blood parameters of laying hens. At 38 weeks of age, Lohmann Brown Lite hens were housed in three-floor cages, divided into three dietary groups (C-control, E1-pumpkin oil (3%), E2-flaxseed oil (3%)). There were housed six hens in one cage. A total 18 hens were monitored. In the control group hens were fed with standard complete feed mixture for laying hens and in the experimental groups by feed mixtures with supplementation of pumpkin or flaxseed oils. Vitamin E was added into feed mixture in the experimental groups. The experiment lasted 52 days. Twelve eggs from each dietary treatment were randomly selected and analyzed. As regards the mineral composition of eggs, only concentrations of calcium after both oil supplementations and zinc after flaxseed oil supplementation in diet were significantly (P<0.05) higher in yolk. Contents of magnesium in yolk and albumen and phosphorus in albumen were markedly (P<0.05) lower in E1 and E2 compared to the control. Also lower (P<0.05) concentrations of potassium after flaxseed oil supplementation in yolk and zinc after both oil supplementation in albumen compared to the control were found. The amounts of other minerals were relatively balanced among groups. Significant (P<0.05) differences among groups in blood parameters of laying hens were observed in mineral and energy profile. Higher (P<0.05) concentrations of calcium, triglycerides and cholesterol in both experimental groups in comparison with control group were found in blood serum of laying hens. The supplementation of oils in diets of laying hens had not notable (P>0.05) influence on enzymatic and protein profile. AbstraktCieľom práce bolo analyzovať vplyv tekvicového a ľanového oleja na úžitkovosť nosníc a nutričné zloženie konzumných vajec. Do pokusu boli zaradené nosnice hybridu Lohmann Brown Lite vo veku 38 týždňov, ktoré boli rozdelené do troch diétnych skupín (C-kontrola, E1-tekvicový olej (3%), E2-ľanový olej (3%)). Nosnice boli ustajnené v trojetážovej klietkovej technológii (6 zvierat v jednej klietke). Spolu bolo monitorovaných 18 nosníc. V kontrolnej skupine nosnice prijímali štandardnú kompletnú kŕmnu zmes a v experimentálnych skupinách bola táto kŕmna zmes obohatená o prídavok tekvicového alebo ľanového oleja. Do pokusných diét bol pridávaný aj vitamín E. Experiment trval 52 dní. Z každej skupiny bolo náhodne vybraných a analyzovaných 12 vajec. Čo sa týka minerálneho zloženia vajec, preukazne (P<0.05) vyšší obsah vápnika bol zaznamenaný po oboch olejových prídavkoch a preukazne vyšší obsah zinku v skupine s ľanovým olejom v porovnaní s kontrolnou skupinou vo vaječnom žĺtku. Obsahy horčíka v žĺtku a bielku a fosforu v bielku boli preukazne (P<0.05) nižšie v E1 a E2 v porovnaní s kontrolou. Pokles (P<0.05) v koncentrácii draslíka po prídavku ľanového oleja v žĺtku a zinku po pridaní oboch olejov v bielku bol pozorovaný v porovnaní so skupinou bez olejového prídavku. Množstvo os...

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Protective effects ofOriganum vulgareethanol extract against cyclophosphamide-induced liver toxicity in mice

Cited by 55 publications

References 31 publications

Effect of a phytogenic additive on blood serum indicator levels and fatty acids profile in fattening turkeys meat

Effect of a phytogenic additive on blood serum indicator levels and fatty acids profile in fattening turkeys meat

Role of the CLOCK protein in liver detoxification

The effect of essential plant oils on mineral composition of egg mass and blood parameters of laying hens

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