“…Throughout the experimental period, all animals were carefully observed once daily for any clinical signs of toxicity according to the Hippocratic screening as follows: general activity, reflexes, response to tail touch and grip, straightening, strength to grab, tremors, Straub tail reaction, sedation, lacrimation, cyanosis, salivation, and piloerection. 15 Body weights were measured daily for dose calculation and statistical analysis at 23,29,36,43, and 50 days of age. In addition, food and water consumption were evaluated on days 23, 29, 36, 43, and 50 after birth.…”