Influences of medium consistency and shoot density on in vitro shoot proliferation of Populus alba � P. grandidentata

Abstract

The in vitro shoot proliferation ofPopulus alba XP. grandidentata was affected by the medium consistency and shoot density, but not by three sizes of vessels. After 4 weeks of culture, the fresh weight and number of shoots per explant on liquid medium were significantly greater than those on agar-solidified medium. In particular, 3.2 shoots, 7 mm or longer per explant, were produced on liquid medium compared with 1.6 shoots per explant or agar-solidified medium. The fresh weight per exp'lant after 4 weeks of culture on liquid medium and agar-solidified medium were 0.68 and 0.25 g, respectively. Increasing the number of shoots per vessel slowed the growth of the explants as measured by fresh weight and the number of shoots produced. There was little difference in the number of shoots produced between vessels with 1 or 2 shoots per vessel, but there were many fewer shoots produced when 3 shoots were placed in each vessel.

Development of a regeneration system via nodal segment culture inVeronica anagallis-aquaticaL. – an amphibious medicinal plant

“…Thus, liquid medium provides better nutrients translocation to the explant and that is why an early response and subsequent growth of the shoots with simultaneous rooting was observed. Similar results had also been reported by Chun et al (1986) and Vinocur et al (2000) in liquid culture studies conducted in Populus alba )Populus grandidentata and Populus tremula, respectively. Furthermore, subsequent rooting also facilitates a direct transfer of plantlet for acclimatization, eliminating root induction phase, thus, reducing the propagation cycle.…”

Effect of Basal Medium, Explants Size and Density on the In Vitro Proliferation and Growth of Date Palm (Phoenix dactylifera L.) Cultivar ‘16-bis’

“…Increasing explants density reduced the proliferation rate per cluster. This result is in good agreement with previous results of Populus alba × P. grandidentata (Chun et al, 1986). However, Kane and Philman (1992) reported that explant density had no effect on regeneration rate per explant in Pontederia cordata L., which shows difference among species.…”

Multiple shoot regeneration from root organ cultures of Populus alba x P. grandidentata

“…Poplars and aspens are gaining importance as forest trees because of their characteristics as pioneer species for new sites, rich potential for short-rotation culture, fast growth rate, and suitability for different planting sites [1,4,7,9,18]. Although poplars can be vegetatively propagated by root suckers, grafting, and the rooting of green shoots, the commercial feasibility of the vegetative production of specific genotypes for planting is limited by the low yields of existing techniques [1].…”

“…Although poplars can be vegetatively propagated by root suckers, grafting, and the rooting of green shoots, the commercial feasibility of the vegetative production of specific genotypes for planting is limited by the low yields of existing techniques [1]. In Populus species, various explant sources have been used for extensive regeneration studies [4,5,6,12,14,16,19,20,21]. In Populus species, various explant sources have been used for extensive regeneration studies [4,5,6,12,14,16,19,20,21].…”