Effect of Basal Medium, Explants Size and Density on the In Vitro Proliferation and Growth of Date Palm (Phoenix dactylifera L.) Cultivar ‘16-bis’

Mazri, Mouaad Amine

doi:10.15835/nsb539089

Cited by 14 publications

(10 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In cv. 16-bis, MS medium showed higher multiplication efficiency than woody plant medium and Nitsch medium (Mazri 2013 ). In the present work, the combination of 825 mg/L NH 4 NO 3 , 1900 mg/L KNO 3 , 220 mg/L CaCl 2 ·2H 2 O, 170 mg/L KH 2 PO 4 and 370 mg/L MgSO 4 ·7H 2 O considerably stimulated the formation of adventitious buds.…”

Section: Discussionmentioning

confidence: 90%

See 1 more Smart Citation

Optimization of medium composition for in vitro shoot proliferation and growth of date palm cv. Mejhoul

et al. 2016

Self Cite

View full text Add to dashboard Cite

The effects of major mineral salts, l-glutamine, myo-inositol and carbon source on shoot bud proliferation of date palm (Phoenix dactylifera L.) cv. Mejhoul were evaluated. Different concentrations of ammonium nitrate (NH4NO3; 550, 825 or 1650 mg/L), potassium nitrate (KNO3; 633.3, 950 or 1900 mg/L), calcium chloride dehydrate (CaCl2·2H2O; 147, 220 or 440 mg/L), potassium dihydrogen phosphate (KH2PO4; 57, 85 or 170 mg/L), magnesium sulfate heptahydrate (MgSO4·7H2O; 123, 185 or 370 mg/L), l-glutamine and myo-inositol (0.5, 1, 1.5 and 2 g/L), sucrose, sorbitol, mannitol or commercial granulated sugar (10, 20, 30, 40 or 50 g/L) were tested. The highest number of shoot buds per explant (18.7) occurred on the medium containing 825 mg/L NH4NO3, 1900 mg/L KNO3, 220 mg/L CaCl2·2H2O, 170 mg/L KH2PO4, 370 mg/L MgSO4·7H2O as well as 1 g/L l-glutamine, 2 g/L myo-inositol and 30 g/L sucrose. The results showed that the frequency of hyperhydricity significantly increased in media containing 1650 mg/L NH4NO3. The concentrations of l-glutamine, myo-inositol and carbon source significantly affected the number of shoot buds per explant. However, they had no effect on hyperhydricity, tissue browning and precocious rooting. Shoots of 4.5–6.0 cm in length were isolated and transferred onto hormone-free media for elongation and rooting. After 3 months, the developed plantlets were successfully transplanted in a glasshouse and over 90 % survived acclimatization.

show abstract

Section: Discussionmentioning

confidence: 90%

“…However, the majority of works focused on the effects of plant growth regulators (PGRs). The effects of other factors such as mineral salts, l -glutamine, myo-inositol and carbon source have been scarcely evaluated for date palm organogenesis (Al-Khateeb 2008a ; Mazri 2013 , 2014 ; Mazri and Meziani 2013 ).…”

Section: Introductionmentioning

confidence: 99%

Optimization of medium composition for in vitro shoot proliferation and growth of date palm cv. Mejhoul

et al. 2016

Self Cite

View full text Add to dashboard Cite

show abstract

“…The highest multiplication ratio occurred when the plant density was lowest (6 buds per vessel). In sugarcane [ 26 ], hosta [ 27 ], garlic [ 28 ], and palm culture [ 29 ], the multiplication increased with lowered plant density. Increasing P from 1.25 mM, which is the concentration of P in the standard MS medium and the lowest concentration tested to 6.25 mM P produced more plants.…”

Section: Resultsmentioning

confidence: 99%

In Vitro Growth of Curcuma longa L. in Response to Five Mineral Elements and Plant Density in Fed-Batch Culture Systems

2015

View full text Add to dashboard Cite

Plant density was varied with P, Ca, Mg, and KNO3 in a multifactor experiment to improve Curcuma longa L. micropropagation, biomass and microrhizome development in fed-batch liquid culture. The experiment had two paired D-optimal designs, testing sucrose fed-batch and nutrient sucrose fed-batch techniques. When sucrose became depleted, volume was restored to 5% m/v sucrose in 200 ml of modified liquid MS medium by adding sucrose solutions. Similarly, nutrient sucrose fed-batch was restored to set points with double concentration of treatments’ macronutrient and MS micronutrient solutions, along with sucrose solutions. Changes in the amounts of water and sucrose supplementations were driven by the interaction of P and KNO3 concentrations. Increasing P from 1.25 to 6.25 mM increased both multiplication and biomass. The multiplication ratio was greatest in the nutrient sucrose fed-batch technique with the highest level of P, 6 buds/vessel, and the lowest level of Ca and KNO3. The highest density (18 buds/vessel) produced the highest fresh biomass at the highest concentrations of KNO3 and P with nutrient sucrose fed-batch, and moderate Ca and Mg concentrations. However, maximal rhizome dry biomass required highest P, sucrose fed-batch, and a moderate plant density. Different media formulations and fed-batch techniques were identified to maximize the propagation and storage organ responses. A single experimental design was used to optimize these dual purposes.

show abstract

“…Moreover, explants between one and three cm prepared from shoot tips of species from Cryptomeria, Sequoia and Chamaecyparis genera of the Cupressaceae family gave better results compared to smaller explants (Isikawa, 1987;Ball, 1987;Kurz, 1989). In general, the effect of explant size on regeneration in plants is well documented (Mazri, 2013;Rehman, 2014;Plaza-Wüthrich et al, 2015). In the present study, the main effects of the three media averaged across explant size, indicated a contrasting response of the number of axillary shoots formed and mean shoot length.…”

Section: Effects Of Explant (Size Orientation) and Media Strengthmentioning

confidence: 99%

Effects of explant, media and growth regulators on in vitro regeneration and antioxidant activity of Juniperus phoenicea

Al-Ramamneh¹,

Daradkeh²,

Rababah³

et al. 2017

Aust J Crop Sci

View full text Add to dashboard Cite

Juniperus phoenicea is an ornamental shrub that is also used to flavor food and to supply medicines and timber. Its micropropagation is of industrial concern and can occur by axillary shoot multiplication. Microcuttings of J. phoenicia were established in vitro on Murashige and Skoog (MS) and Rugini Olive (OM) media in glass tubes (25 mm x 150 mm). Factors studied were explant length (0.5 or 1.5 cm) and orientation (horizontal or vertical), media strength (OM, ½OM, MS, ½MS) and the following growth regulators: the anti-auxin 2,3,5-triiodobenzoic acid (TIBA), the cytokinins 6-benzyladenine (BA) and thidiazuron (TDZ), and the growth retardant daminozide (DM). Microcuttings placed vertically on the surface of OM, ½OM and ½MS media without hormones exhibited axillary bud differentiation, but they were swollen and turned brown one month later when placed horizontally on the medium surface. The number of shoots, averaged across OM, ½OM and ½MS media, was significantly higher from longer (1.5 cm) than shorter (0.5 cm) microcuttings (4.11 versus 1.57 shoots microcutting -1 ) after 60 days of culture. TIBA or DM at 0.1 mg l -1 included in OM medium enhanced leaf differentiation, callus induction and formation of adventitious shoots over three months from 0.5 cm long microcuttings taken from in vitro shoots. The formation of adventitious shoots was sporadic and occurred at a rate of 1 shoot microcutting -1 in the presence of 0.1 mg l -1 DM. OM supplemented with 0.1 mg l -1 TIBA resulted in significantly the highest leaf differentiation (55 leaves microcutting -1 ), with a rooting rate of 40%. Contents of phenols, flavonoids and antioxidants were compared for cuttings from young seedlings, callus, in vitro shoots, and seeds. Antioxidant activity was significantly the highest for shoots grown on hormone-free OM medium and callus maintained on OM medium containing 0.1 mg l -1 2,4-D (94.7 and 94.3% inhibition of DPPH free radicals, respectively). Thus, different routes for in vitro regeneration of J. phoenicea can be of potential use for many biotechnological, pharmaceutical and food industry applications.

show abstract

Effect of Basal Medium, Explants Size and Density on the In Vitro Proliferation and Growth of Date Palm (Phoenix dactylifera L.) Cultivar ‘16-bis’

Cited by 14 publications

References 17 publications

Optimization of medium composition for in vitro shoot proliferation and growth of date palm cv. Mejhoul

Optimization of medium composition for in vitro shoot proliferation and growth of date palm cv. Mejhoul

In Vitro Growth of Curcuma longa L. in Response to Five Mineral Elements and Plant Density in Fed-Batch Culture Systems

Effects of explant, media and growth regulators on in vitro regeneration and antioxidant activity of Juniperus phoenicea

Contact Info

Product

Resources

About