Multiple shoot regeneration from root organ cultures of Populus alba x P. grandidentata

Son, Sung Ho; Hall, Richard B.

doi:10.1007/bf00034757

Cited by 26 publications

(10 citation statements)

References 15 publications

(18 reference statements)

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“…After ;2 weeks of incubation in the dark at 25°C, transgenic roots appeared from wounded sites (Supplemental Figure 10A). These roots were screened using a fluorescent stereomicroscope (Leica M165 FC), and those visibly expressing DsRed were excised and placed on antibiotic medium containing 15 mM trans-zeatin (Cayman Chemical) to induce shoot formation as described previously (Son and Hall, 1990). Shoots appeared within as little as 2 months and were again screened using the same fluorescent stereomicroscope (Supplemental Figures 10B and 14).…”

Section: Agrobacterium Rhizogenes-mediated Populus Transformationmentioning

confidence: 99%

The Ectomycorrhizal Fungus Laccaria bicolor Produces Lipochitooligosaccharides and Uses the Common Symbiosis Pathway to Colonize Populus Roots

et al. 2019

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0000-0002-3128-9439 (J.-M.A.)Mycorrhizal fungi form mutualistic associations with the roots of most land plants and provide them with mineral nutrients from the soil in exchange for fixed carbon derived from photosynthesis. The common symbiosis pathway (CSP) is a conserved molecular signaling pathway in all plants capable of associating with arbuscular mycorrhizal fungi. It is required not only for arbuscular mycorrhizal symbiosis but also for rhizobia-legume and actinorhizal symbioses. Given its role in such diverse symbiotic associations, we hypothesized that the CSP also plays a role in ectomycorrhizal associations. We showed that the ectomycorrhizal fungus Laccaria bicolor produces an array of lipochitooligosaccharides (LCOs) that can trigger both root hair branching in legumes and, most importantly, calcium spiking in the host plant Populus in a CASTOR/POLLUX-dependent manner. Nonsulfated LCOs enhanced lateral root development in Populus in a calcium/calmodulin-dependent protein kinase (CCaMK)-dependent manner, and sulfated LCOs enhanced the colonization of Populus by L. bicolor. Compared with the wildtype Populus, the colonization of CASTOR/POLLUX and CCaMK RNA interference lines by L. bicolor was reduced. Our work demonstrates that similar to other root symbioses, L. bicolor uses the CSP for the full establishment of its mutualistic association with Populus.

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Section: Agrobacterium Rhizogenes-mediated Populus Transformationmentioning

confidence: 99%

The Ectomycorrhizal Fungus Laccaria bicolor Produces Lipochitooligosaccharides and Uses the Common Symbiosis Pathway to Colonize Populus Roots

et al. 2019

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“…These important traits, coupled with the existence of a library of full-length enriched expressed sequence tags [1] and availability of the genome sequence of Populus tricocarpa, have established poplar as a model system in molecular genetics [2][3][4][5] and tree physiology [6][7][8] studies. Furthermore, these rapidly growing plants, which have the potential to enhance wood supplies for the plywood, hardboard, pulp, and paper industries, represent a commercially important resource [9].…”

Section: Introductionmentioning

confidence: 99%

An Improved System for Shoot Regeneration from Stem Explants of Lombardy Poplar (Populus nigra L. var. italica Koehne)

Biswas¹,

Mohri²,

Kogawara³

et al. 2012

AJPS

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We developed a system for the regeneration of Lombardy poplar (Populus nigra L. var. italica) shoots from internodal stem explants. Using this system, shoots regenerated from 87% of the stem explants placed on Murashige and Skoog (MS) medium supplemented with 0.1 mg/L indole-3-acetic acid and 0.5 mg/L benzylaminopurine without undergoing callus formation. About 80% of the in vitro regenerated shoots developed roots on MS medium supplemented with 0.5 mg/L indole-3-butyric acid and 0.02 mg/L 1-naphthylacetic acid. Well-rooted seven-to eight-week-old regenerated plants could be transferred to soil for further growth and the survival rate of such plants after three weeks was 88%. The protocol presented here is simple and economical because it does not rely on pre-incubation in callus induction medium or repeated subculture in shoot induction medium containing trans-zeatin, an expensive substance. The in vitro regeneration system presented here could be used for evaluation of radiation sensitivity for Lombardy poplar tissues.

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“…Because of their high regeneration capacity (Park & Son 1988;Park & Son 1989;Russell & McCown 1986) and commercial value (Hall et al 1982), there are numerous reports of rapid in vitro multiplication methods for various explant sources of Populus species. To minimize the cost of tissue cultured plants, a two-step method was successfully adapted to this species (Ahuja 1987;Son & Hall 1990a). Nevertheless, mass propagation of Populus species is still limited in commercial use because of the difficulties in large-scale synchronization of transplanting propagules to the nursery for an optimal growing season, possible genetic instability from long-term subcultures, and limited space and labor (Aitken-Christie & Singh 1987).…”

Section: Introductionmentioning

confidence: 99%

Cold storage of in vitro cultures of hybrid poplar shoots (Populus alba L. x P. grandidentata Michx.)

Son

Chun

Hall

1991

Plant Cell Tiss Organ Cult

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Shoot cultures of P. alba x P. grandidentata 'Crandon' were maintained for more than 5 years at 4°C with minimal growth. The highest survival after 2 years and 5 years of cold storage were 70% and 25% respectively using 1-month of pre-storage culture on MS medium containing 1.33/zMBA. When 5-year-old cold-stored shoot cultures were transferred to the greenhouse, color variations were observed. The frequencies of albino and red pigmented plants were 0.25% and 12.8%, respectively. A rosette type growth pattern was also observed on 0.3% of the long-term cold-stored plants. During long-term cold storage there were local disruption of cambium connections and the accumulations of chemicals in some cells, as observed by light microscopy.

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Multiple shoot regeneration from root organ cultures of Populus alba x P. grandidentata

Cited by 26 publications

References 15 publications

The Ectomycorrhizal Fungus Laccaria bicolor Produces Lipochitooligosaccharides and Uses the Common Symbiosis Pathway to Colonize Populus Roots

The Ectomycorrhizal Fungus Laccaria bicolor Produces Lipochitooligosaccharides and Uses the Common Symbiosis Pathway to Colonize Populus Roots

An Improved System for Shoot Regeneration from Stem Explants of Lombardy Poplar (Populus nigra L. var. italica Koehne)

Cold storage of in vitro cultures of hybrid poplar shoots (Populus alba L. x P. grandidentata Michx.)

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