Influence of traditional medicines on the activity of keratinocytes in wound healing: an in-vitro study

Abstract

Natural medicinal systems such as Ayurveda and folk medicine has remedies for wound management. However, the exact cellular and extracellular mechanisms involved in the healing process and its influence on keratinocytes is less discussed. Therefore, the present study was designed to evaluate the effect of certain natural wound healing medicines on the biology of the keratinocytes/HaCaT cells. Test materials such as honey (H), ghee (G), aqueous extracts of roots of Glycyrrhiza glabra (GG) and leaves of Nerium indicum (NI) were considered. The HaCaT cells were treated with the test materials singly and in combinations (H+G, all combined [Tot]) for a specific period (24, 48, and 72 hours). The cells were then subjected to cytotoxicity/proliferation and migration/scratch assays. All the test materials, except NI, were non-cytotoxic and showed increased cell proliferation at variable concentrations. Significant observations were made in the groups treated with honey

Cytotoxicity and Wound Closure Evaluation in Skin Cell Lines after Treatment with Common Antiseptics for Clinical Use

“…HFs and HaCaT cells were seeded in 12-well plates (Thermo Fisher Scientific, Carlsbad, CA, USA) at a density of 10,000 cells/cm 2 . When the cells were confluent and adherent to the plate, the DFM was removed and each well was scratched with a sterile 10 µL pipette tip to simulate wound formation [12]. Cells were then treated for three minutes with the different antiseptics mentioned above, at a concentration of 1% of the clinically used stock solution.…”

“…Control cells were left untreated. Cell debris and traces of the antiseptic solution were removed by washing with DPBS [12]. Then, the cells were incubated and images were taken of the scraped area of each well until the control was completely closed.…”

Isoegomaketone from Perilla frutescens (L.) Britt Stimulates MAPK/ERK Pathway in Human Keratinocyte to Promote Skin Wound Healing

“…Natural products and botanical drugs have been traditionally used as healing substances to assist wound healing and treat skin diseases since prehistoric times [ 17 , 18 ]. Numerous studies have been performed to find appropriate alternative remedies, derived from natural products, to treat wounds [ 19 ]. Perilla frutescens (L.) Britt is an annual herbal plant belonging to the Lamiaceae family, which has been used as a botanical drug to treat disorders such as depression-related diseases, anxiety, asthma, chest stuffiness, nausea, fever, headache, constipation, and indigestion.…”

A Review of Wound Healing Mechanisms of Natural Products in Keratinocyte Cells

“…The antioxidant effect of some GG constituents, such as glycyrrhizin and glabridin, may also help enhance the wound healing ability of keratinocytes (Figure 15). 45 G. glabra aqueous extract ointment could significantly (p ≤ 0.05) decrease the level of the wound site, total cell, macrophage, lymphocyte, and neutrophil, and enhance the level of wound contracture, fibrocyte, hexuronic acid, and hydroxyproline as compared with the basal ointment and control groups. 46 Calabrian honey BL1 (multifloral) and BL5 (orange) honey showed the best healing properties among the five kinds of honey tested.…”