Isoegomaketone from Perilla frutescens (L.) Britt Stimulates MAPK/ERK Pathway in Human Keratinocyte to Promote Skin Wound Healing

Kim, Ye-Ram; Nam, Bomi; Han, Ah‐Reum; Kim, Jin-Baek; Jin, Chang Hyun

doi:10.1155/2021/6642606

Cited by 10 publications

(8 citation statements)

References 42 publications

(32 reference statements)

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“…According to Veith et al, reactive free radicals activate the vascular endothelial growth factor during angiogenesis. Furthermore, reactive spices are used for wound sterilization (20)(21)(22). In a study by Isbary et al exploring argon plasma in chronic wound healing, no abnormal side effects were observed in tissues (23) and, similar to our study, visceral tissues were not damaged by helium plasma radiation during histopathological examination.…”

Section: Discussionsupporting

confidence: 88%

Effects of Cold Atmospheric Plasma on Infectious Diabetic Wound Healing in Rat Models

Dehghanpisheh

Jahandideh

Asghari

et al. 2023

Crescent J Med Biol Sci

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Objectives: Patients with diabetes often suffer from chronic wounds which can occur due to the impairment of wound healing in these patients. Scientists have been trying to address this issue by using wide spectrum of antibiotics and drugs. However, overusing antibiotics in recent years has led to antibiotic resistant crisis; therefore, there is an urgent need to develop new approaches in order for controlling microbial infections in diabetic patients. This study aimed to determine if cold plasma was effective in wound healing in the infectious diabetic conditions. Material and Methods: In this experimental study, 40 adult male rats with diabetes infected with Staphylococcus aureus were included. The animals were randomly divided into two groups of 20 rats and four sub-groups (i.e., day 3rd, day 7th, day 14th, and day 21st). The rats in the treatment group were exposed to helium plasma irradiated for 3 minutes per day for 21 days. Wound healing in the samples was evaluated using five healing indexes including epithelium formation, Inflammatory cells, new vascularization, fibroblast, and collagen formation. Results: A significant difference (P<0.05) was found between the indexes in the plasma radiation group compared to the control group. The results showed that the speed of wound healing in the group treated with cold helium gas plasma was higher than that in the control group. Collagen formation was always completed faster in the helium plasma group, indicating the positive effects of helium plasma on infected diabetic wounds. Conclusions: Helium plasma was remarkable effective in healing wound and controlling infections in diabetic rats.

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Section: Discussionsupporting

confidence: 88%

Effects of Cold Atmospheric Plasma on Infectious Diabetic Wound Healing in Rat Models

Dehghanpisheh

Jahandideh

Asghari

et al. 2023

Crescent J Med Biol Sci

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“…The results were supported by the ability of PFP in promoting re-epithelialization in a wound healing assay. Previous papers reported the beneficial effect of P. frutescens on the intestinal epithelial barrier by targeting intestinal permeability [ 53 ], whereas Ye-Ram Kim et al showed the increased proliferation and migration of HaCaT cells treated with isoegomaketone, a phytochemical isolated from P. frutescens leaves [ 54 ]. PFP was not as effective in modulating human fibroblast metabolism as it was in human keratinocytes, and it was devoid of capacity in modulating pro-collagen I and elastin synthesis.…”

Section: Discussionmentioning

confidence: 99%

A Novel Perilla frutescens (L.) Britton Cell-Derived Phytocomplex Regulates Keratinocytes Inflammatory Cascade and Barrier Function and Preserves Vaginal Mucosal Integrity In Vivo

Pressi¹,

Rigillo

Governa

et al. 2023

Pharmaceutics

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In the last years, the medicinal plant Perilla frutescens (L.) Britton has gained scientific interest because leaf extracts, due to the presence of rosmarinic acid and other polyphenols, have shown anti-allergic and skin protective potential in pre-clinical studies. Nevertheless, the lack of standardized extracts has limited clinical applications to date. In this work, for the first time, a standardized phytocomplex of P. frutescens, enriched in rosmarinic acid and total polyphenols, was produced through innovative in vitro cell culture biotechnology and tested. The activity of perilla was evaluated in an in vitro inflammatory model of human keratinocytes (HaCaT) by monitoring tight junctions, filaggrin, and loricrin protein levels, the release of pro-inflammatory cytokines and JNK MAPK signaling. In a practical health care application, the perilla biotechnological phytocomplex was tested in a multilayer model of vaginal mucosa, and then, in a preliminary clinical observation to explore its capacity to preserve vaginal mucosal integrity in women in peri-menopause. In keratinocytes cells, perilla phytocomplex demonstrated to exert a marked activity in epidermis barrier maintenance and anti-inflammatory effects, preserving tight junction expression and downregulating cytokines release through targeting JNK activation. Furthermore, perilla showed positive effects in retaining vaginal mucosal integrity in the reconstructed vaginal mucosa model and in vivo tests. Overall, our data suggest that the biotechnological P. frutescens phytocomplex could represent an innovative ingredient for dermatological applications.

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“…Among the principal activated signaling pathways in wound healing are MAPKs [35,36]. MAPK14 was shown to be specifically activated in diabetic patients [37] and could thus be activated by ES, as ES was shown to promote cell proliferation and migration via the activation of MAPKs [38,39].…”

Section: Discussionmentioning

confidence: 99%

Effect of Electrical Stimulation on Diabetic Human Skin Fibroblast Growth and the Secretion of Cytokines and Growth Factors Involved in Wound Healing

Abedin-Do

Zhang

Douville

et al. 2021

Biology

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Diabetic foot ulcers are indicative of an impaired wound healing process. This delay may be resolved through electrical stimulation (ES). The goal of the present study was to evaluate the effect of ES on diabetic fibroblast adhesion and growth, and the secretion of cytokines and growth factors. Diabetic human skin fibroblasts (DHSF) were exposed to various intensities of direct current ES (100, 80, 40 and 20 mV/mm). The effect of ES on fibroblast adhesion and growth was evaluated using Hoechst staining, MTT and trypan blue exclusion assays. The secretion of cytokine and growth factor was assessed by cytokine array and ELISA assay. The long-term effects of ES on DHSF shape and growth were determined by optical microscopy and cell count. We demonstrated that ES at 20 and 40 mV/mm promoted cell adhesion, viability and growth. ES also decreased the secretion of pro-inflammatory cytokines IL-6 and IL-8 yet promoted growth factor FGF7 secretion during 48 h post-ES. Finally, the beneficial effect of ES on fibroblast growth was maintained up to 5 days post-ES. Overall results suggest the possible use of low-intensity direct current ES to promote wound healing in diabetic patients.

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Isoegomaketone from Perilla frutescens (L.) Britt Stimulates MAPK/ERK Pathway in Human Keratinocyte to Promote Skin Wound Healing

Cited by 10 publications

References 42 publications

Effects of Cold Atmospheric Plasma on Infectious Diabetic Wound Healing in Rat Models

Effects of Cold Atmospheric Plasma on Infectious Diabetic Wound Healing in Rat Models

A Novel Perilla frutescens (L.) Britton Cell-Derived Phytocomplex Regulates Keratinocytes Inflammatory Cascade and Barrier Function and Preserves Vaginal Mucosal Integrity In Vivo

Effect of Electrical Stimulation on Diabetic Human Skin Fibroblast Growth and the Secretion of Cytokines and Growth Factors Involved in Wound Healing

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