Influence of three laser wavelengths on human fibroblasts cell culture

Crișan, Bogdan; Şoriţău, Olga; Băciuţ, Mihaela; Câmpian, Radu Septimiu; Crisan, Liana; Băciuț, Grigore

doi:10.1007/s10103-012-1084-5

Cited by 22 publications

(20 citation statements)

References 27 publications

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“…Apart from energy density, studies also focused on the influence of different wavelength on fibroblast proliferation. Crisan et al (14) compared the effects of 830, 980, and 2,940 nm lasers (5.5 J/cm 2 ) on human skin fibroblasts by MTT assay and apoptosis assay. They demonstrated that both 830 and 980 nm significantly stimulated cell proliferation at 24, 48, 72 h post-irradiation but 2,940 nm inhibited cell proliferation and promoted apoptosis.…”

Section: Proliferation and Differentiationmentioning

confidence: 99%

Review on the Cellular Mechanisms of Low-Level Laser Therapy Use in Oncology

Tam

Ramkumar

et al. 2020

Front. Oncol.

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Photobiomodulation (PBM) using low-level laser therapy (LLLT) is a treatment that is increasingly used in oncology. Studies reported enhancement of wound healing with reduction in pain, tissue swelling and inflammatory conditions such as radiation dermatitis, oral mucositis, and lymphedema. However, factors such as wavelength, energy density and irradiation frequency influence the cellular mechanisms of LLLT. Moreover, the effects of LLLT vary according to cell types. Thus, controversy arose as a result of poor clinical response reported in some studies that may have used inadequately planned treatment protocols. Since LLLT may enhance tumor cell proliferation, these will also need to be considered before clinical use. This review aims to summarize the current knowledge of the cellular mechanisms of LLLT by considering its effects on cell proliferation, metabolism, angiogenesis, apoptosis and inflammation. With a better understanding of the cellular mechanisms, bridging findings from laboratory studies to clinical application can be improved.

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Section: Proliferation and Differentiationmentioning

confidence: 99%

Review on the Cellular Mechanisms of Low-Level Laser Therapy Use in Oncology

Tam

Ramkumar

et al. 2020

Front. Oncol.

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“…It is difficult to compare our results with observations of other studies in the literature, because most studies have reported only one or two different single wavelength and treatment design varies depending on individual studies [10,11]. However, some studies have been performed in a similar manner to our study [12,20,21]. Bogdan Crisan has compared near-infrared laser light at wavelength of 830 nm, 980 nm, and 2,940 nm.…”

Section: Discussionmentioning

confidence: 88%

“…It has been reported that 780 nm laser light can up-regulate the production of basic fibroblastic growth factor (bFGF) more than 660 nm visible red laser [12]. Diabetic fibroblasts irradiated at 830 nm have shown more bFGF release compared to those irradiated at 632.8 nm [20].…”

Section: Discussionmentioning

confidence: 99%

Effect of Low-Level Laser Therapy on Proliferation and Collagen Synthesis of Human Fibroblasts in Vitro

Yang

Tan

et al. 2018

J Wound Management Res

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The prevalence of chronic wounds is increasing dramatically, as the populations of industrialized countries age and become more sedentary. Chronic wounds that respond poorly to conventional treatment, making them very difficult to manage [1]. It is well-known that fibroblasts provide desired growth factors and other substances to accelerate wound healing. However, fibroblasts from chronic wounds such as diabetic ulcers have been commonly demonstrated a lower rate of proliferation when compared with healthy ones [2]. Therefore, it is significant to increase fibroblast activity for accelerating wound healing. The care of chronic wounds has become its own specialty, with providers often using advanced therapies, including growth factors, extracellular matrices (ECMs), engineered skin, and negative pressure wound therapy (NPWT) [3]. Low-level laser therapy (LLLT) is a nonthermal technology that can be used to modulate cellular activity through light irradiation at specific pulse sequences [4]. In vitro and in vivo studies have been performed previously to determine the effect of LLLT on wound healing. However the results were inconsistent. Using a variety of red, blue, yellow, and infrared light wavelength, it has been reported that LLLT can

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“…Experimental in vitro studies conducted by our research team in recent years related to laser radiation effects on human fibroblasts involved in healing and tissue regeneration processes [ 13 , 14 ] enabled us to develop new protocols of irradiation on other human cell types such as osteoblasts.…”

Section: Background and Aimsmentioning

confidence: 99%

The Influence of Laser Radiation on Human Osteoblasts Cultured on Nanostructured Composite Substrates

Crisan

Şoriţău

Băciuţ

et al. 2015

Medicine and Pharmacy Reports

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Background and aimsCarbon-based nanomaterials such as carbon nanotubes, graphene oxide and graphene have been explored by researchers as well as the industry. Graphene is a new nanomaterial which has commercial and scientific advantages. Laser therapy has proven highly useful in biomedicine, with the use of different laser types and energies for distinct purposes. The low level laser therapy (LLLT) can have anti-inflammatory, analgesic and biostimulant effects. Recent research has shown that laser radiation has different effects on osteoblasts. The aim of this study was to identify the influence of laser radiation on human osteoblastic cells cultured on nanostructured composite substrates.Materials and methodsFour types of substrates were created using colloidal suspensions of nanostructured composites in PBS at a concentration of 30 μg/ml. We used human osteoblasts isolated from patella bone pieces harvested during arthroplasty. Irradiation of osteoblasts cultured on nanostructured composite substrates was made with a semiconductor laser model BTL-10 having a wavelength of 830 nm. The proliferation activity of osteoblast cells was assessed using the MTT assay. After laser irradiation procedure the viability and proliferation of osteoblast cells were analyzed using fluorescein diacetate (FDA) staining.Results The osteoblast cells viability and proliferation were evaluated with MTT assay at 30 minutes, 24 hours, 5 days and 10 days after laser irradiation. In the first 30 minutes there were no significant differences between the irradiated and non-irradiated cells. At 24 hours after laser irradiation procedure a significant increase of MTT values in case of irradiated osteoblasts cultivated on nanostructured hydroxyapatite, nanostructured hydroxyapatite with gold nanoparticles and 1.6% and 3.15% graphenes composites substrates was observed. A more marked proliferation rate was observed after 10 days of irradiation for irradiated osteoblasts seeded on nanostructured hydroxyapatite with gold nanoparticles and graphenes containing substrate. Using FDA staining we obtained very similar results with MTT test.Conclusions The association between the 830 nm laser irradiation of osteoblasts and their long-term cultivation of the nanostructured composite substrates induces the cell proliferation and differentiation and therefore it will be a useful alternative for bone regeneration therapy.

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Influence of three laser wavelengths on human fibroblasts cell culture

Cited by 22 publications

References 27 publications

Review on the Cellular Mechanisms of Low-Level Laser Therapy Use in Oncology

Review on the Cellular Mechanisms of Low-Level Laser Therapy Use in Oncology

Effect of Low-Level Laser Therapy on Proliferation and Collagen Synthesis of Human Fibroblasts in Vitro

The Influence of Laser Radiation on Human Osteoblasts Cultured on Nanostructured Composite Substrates

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