Effect of Low-Level Laser Therapy on Proliferation and Collagen Synthesis of Human Fibroblasts in Vitro

Ma, Huiling; Yang, Jong-Phil; Tan, Rui Ken; Lee, Hyup-Woo; Han, Seung Kyu

doi:10.22467/jwmr.2018.00283

Cited by 27 publications

(10 citation statements)

References 23 publications

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“…They demonstrated that both 830 and 980 nm significantly stimulated cell proliferation at 24, 48, 72 h post-irradiation but 2,940 nm inhibited cell proliferation and promoted apoptosis. Ma et al (15) reported increased human fibroblast proliferation and collagen synthesis by 830 or 635 nm and 830 nm dual wavelength (60 J/cm 2 ) LLLT, but 635 nm alone did not produce significant fibroblast proliferation or collagen synthesis. Mignon et al (16) reported that a short-wavelength of light (<530 nm) inhibited the metabolic activity of human dermal fibroblasts, but not with longer wavelengths of 550-850 nm.…”

Section: Proliferation and Differentiationmentioning

confidence: 99%

Review on the Cellular Mechanisms of Low-Level Laser Therapy Use in Oncology

Tam

Ramkumar

et al. 2020

Front. Oncol.

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Photobiomodulation (PBM) using low-level laser therapy (LLLT) is a treatment that is increasingly used in oncology. Studies reported enhancement of wound healing with reduction in pain, tissue swelling and inflammatory conditions such as radiation dermatitis, oral mucositis, and lymphedema. However, factors such as wavelength, energy density and irradiation frequency influence the cellular mechanisms of LLLT. Moreover, the effects of LLLT vary according to cell types. Thus, controversy arose as a result of poor clinical response reported in some studies that may have used inadequately planned treatment protocols. Since LLLT may enhance tumor cell proliferation, these will also need to be considered before clinical use. This review aims to summarize the current knowledge of the cellular mechanisms of LLLT by considering its effects on cell proliferation, metabolism, angiogenesis, apoptosis and inflammation. With a better understanding of the cellular mechanisms, bridging findings from laboratory studies to clinical application can be improved.

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Section: Proliferation and Differentiationmentioning

confidence: 99%

Review on the Cellular Mechanisms of Low-Level Laser Therapy Use in Oncology

Tam

Ramkumar

et al. 2020

Front. Oncol.

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“…Recently, applications of photomedicine utilizing low level laser therapy (LLLT) as a nonsurgical technique have gained great attention for accelerating the wound healing procedure [10]. It has been shown that LLLT may stimulate cell proliferation, maintaining DNA integrity and the repair of damaged DNA whilst as low energy laser has negligible thermal effects also [10][11][12]. Blue light (wavelength range from 400 nm to 480 nm) which is part of the visible light spectrum is UV-free irradiation having fewer harmful side effects on mammalian cells compared with ultraviolet (UV) irradiation; since irradiation with blue light only reveals toxic effects at high dosages [13].…”

Section: Introductionmentioning

confidence: 99%

Combination treatment of dendrosomal nanocurcumin and low-level laser therapy develops proliferation and migration of mouse embryonic fibroblasts and alter TGF-β, VEGF, TNF-α and IL-6 expressions involved in wound healing process

et al. 2021

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Introduction Pressure ulcer (PU) is known as the third most costly disorder usually caused by prolonged pressure and stagnation in various parts of the body. Although several therapeutic approaches are employing, obstacles in appropriate healing for skin lesions still exist which necessitates new practical alternative or adjunctive treatments. Low level laser therapy (LLLT) as one of the mentioned new strategies have gained attention. Besides, curcumin is an herbal medicine extracted from turmeric with anti-inflammatory and antioxidative properties with promising beneficial therapeutic effects in wound healing. Employing dendrosomal nanoparticles, we overcome the hydrophobicity of curcumin in the present study. We hypothesized that combination treatment of DNC+LLLT (450 nm) simultaneously may promote the wound healing process. Material and methods MTT assay, PI staining followed by flowcytometry, scratch assay and intracellular ROS measurement were used to investigate the effects caused by DNC and LLLT (450 nm) alone and in combination, on proliferation, cell cycle, migration and oxidative stress mouse embryonic fibroblast cells, respectively. The levels of growth factors and pro-inflammatory cytokines were evaluated by qRT-PCR and ELISA. Results Our results indicated that combination exposure with DNC and LLLT leads to increased proliferation and migration of MEFs as well as being more efficient in significantly upregulating growth factors (TGF-β, VEGF) and decline in inflammatory cytokines (TNF-α, IL-6). Moreover, findings of this research provide persuasive support for the notion that DNC could reduce the LLLT-induced enhancement in intracellular ROS in mouse embryonic fibroblasts. Conclusion Concurrent exposure to anti-oxidant concentrations of DNC and LLLT enriched S phase entry and therefor increased proliferation as well as migration on MEFs through regulating the expression levels growth factors and shortening the inflammatory phase by modulating of cytokines. It should be noted that DNC were able to reduce the laser-induced oxidative stress, during wound healing, representing an informative accompaniment with LLLT.

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“…Recent research suggested the therapeutic activity of light may be effective at much lower energy levels than those used clinically [22,24]. Weiss et al [25] showed clinical improvements in photo‐damaged skin when used 0.1 J/cm 2 to treat every other day with 590 nm pulsed LED light for 4 weeks.…”

Section: Introductionmentioning

confidence: 99%

Low‐level red plus near infrared lights combination induces expressions of collagen and elastin in human skin in vitro

Seo

Kim

et al. 2021

Intern J of Cosmetic Sci

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Objective Light therapy has attracted medical interests as a safe, alternative treatment for photo‐ageing and photo‐damaged skin. Recent research suggested the therapeutic activity of red and infrared (IR) lights may be effective at much lower energy levels than those used clinically. This study was to evaluate the efficacy of low‐level red plus near IR light emitting diode (LED) combination on collagen and elastin and ATP production. Methods Human dermal fibroblasts or skin tissues were irradiated daily by red (640 nm) plus near IR (830 nm) LED lights combination at 0.5 mW/cm2 for 10 minutes (0.3 J/cm2). qPCR, ELISAs or histology were used to determine the gene and protein expressions. Fluorescent measurement was used to assess crosslinks of collagen and elastic fibres. ATP production was evaluated by ATP assay. Results Treatment of human fibroblast cell cultures with low‐level red plus near IR lights combination was found to significantly increase LOXL1, ELN and COL1A1 and COL3A1 gene expressions as well as the synthesis of the procollagen type I and elastin proteins. Treating human skin explants with low‐level red plus near IR lights combination similarly induced significant increases in the same gene expressions, type III collagen and elastic fibre formation and crosslinks. ATP production was increased in human dermal fibroblasts after red plus near IR lights combination treatment. Conclusion Low‐level red plus near IR lights combination stimulated the production of collagen and elastin production associated with anti‐ageing benefits. These findings suggest that low‐level red plus near IR LED light combination may provide an effective treatment opportunity for people with photo‐aged skin.

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Effect of Low-Level Laser Therapy on Proliferation and Collagen Synthesis of Human Fibroblasts in Vitro

Cited by 27 publications

References 23 publications

Review on the Cellular Mechanisms of Low-Level Laser Therapy Use in Oncology

Review on the Cellular Mechanisms of Low-Level Laser Therapy Use in Oncology

Combination treatment of dendrosomal nanocurcumin and low-level laser therapy develops proliferation and migration of mouse embryonic fibroblasts and alter TGF-β, VEGF, TNF-α and IL-6 expressions involved in wound healing process

Low‐level red plus near infrared lights combination induces expressions of collagen and elastin in human skin in vitro

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