Low‐level red plus near infrared lights combination induces expressions of collagen and elastin in human skin in vitro

Abstract

Objective Light therapy has attracted medical interests as a safe, alternative treatment for photo‐ageing and photo‐damaged skin. Recent research suggested the therapeutic activity of red and infrared (IR) lights may be effective at much lower energy levels than those used clinically. This study was to evaluate the efficacy of low‐level red plus near IR light emitting diode (LED) combination on collagen and elastin and ATP production. Methods Human dermal fibroblasts or skin tissues were irradiated daily by red (640 nm) plus near IR (830 nm) LED lights combination at 0.5 mW/cm2 for 10 minutes (0.3 J/cm2). qPCR, ELISAs or histology were used to determine the gene and protein expressions. Fluorescent measurement was used to assess crosslinks of collagen and elastic fibres. ATP production was evaluated by ATP assay. Results Treatment of human fibroblast cell cultures with low‐level red plus near IR lights combination was found to significantly increase LOXL1, ELN and COL1A1 and COL3A1 gene expressions as well as the synthesis of the procollagen type I and elastin proteins. Treating human skin explants with low‐level red plus near IR lights combination similarly induced significant increases in the same gene expressions, type III collagen and elastic fibre formation and crosslinks. ATP production was increased in human dermal fibroblasts after red plus near IR lights combination treatment. Conclusion Low‐level red plus near IR lights combination stimulated the production of collagen and elastin production associated with anti‐ageing benefits. These findings suggest that low‐level red plus near IR LED light combination may provide an effective treatment opportunity for people with photo‐aged skin.

Design and realization of a dual-wavelength low level light therapy for acne and face rejuvenation treatment

“…This was consistent with Opel's finding [7] showed that the 630-700 nm red light has a beneficial effect on skin rejuvenation. This is consistent with the reports by Kim et al [9] and Lee et al [10], and the 640 nm red light combined with lowintensity IR light 830 nm was found to be effective in treating photo-aged skin [11]. Papageorgiou et al [12] presented a combination of 415 nm blue light and 633 nm red light to improve the efficacy of acne treatment of 24 patients with mild to moderate facial acne.…”

Repeated Low-level Red-light Therapy: The Next Wave in Myopia Management?

“…For example, it could be that the strong dose of brief periods of low-level red-light therapy is producing irreversible damage at the cellular level to the structure and physiology of the posterior eye components involved in signaling eye growth. Red light stimulation has been shown to increase mRNA expression and production of several types of collagen in different cell types 80,81 . This increase in the production of collagen may contribute to the changes in ocular structure observed in low-level red-light subjects.…”

“…Red light stimulation has been shown to increase mRNA expression and production of several types of collagen in different cell types. 80,81 This increase in the production of collagen may contribute to the changes in ocular structure observed in low-level red-light subjects. It must be noted that all these possibilities are merely speculations at this point.…”

5G Electromagnetic Radiation Attenuates Skin Melanogenesis In Vitro by Suppressing ROS Generation

“…In particular, the impact of 5G electromagnetic radiation (EMR) on human skin has been drawing attention [ 4 , 5 , 6 ], since the skin interfaces between the body and the external environment and serves as the primary target tissue for EMR from the environment. Indeed, the EMR such as ultraviolet, visible light like blue light and infrared radiation affect the skin physiology significantly [ 7 , 8 , 9 , 10 ], signifying that 5G EMR may also have effects on the skin. Also, it is highly probable that the skin may be the primary target tissue of 5G EMR since 28 GHz EMR from the environment cannot penetrate into our body deeper than 2 mm [ 11 ].…”