Influence of the stationary phase on the stability of thalidomide and comparison of different methods for the quantification of thalidomide in tablets using high-temperature liquid chromatography

Giegold, Sascha; Holzhauser, Maren; Kiffmeyer, Thekla K.; Tuerk, Jochen; Teutenberg, Thorsten; Rosenhagen, Maike; Hennies, Dorothea; Hoppe-Tichy, Torsten; Wenclawiak, Bernd W.

doi:10.1016/j.jpba.2007.11.043

Cited by 20 publications

(9 citation statements)

References 44 publications

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“…Previous reports suggested that degradation of pharmaceutical target molecules was temperature-, residence time-, and even stationary phasedependent [63,68]. In this work, we were not able to detect any noticeable changes in the status of analyte oxidation, deamidation, dephosphorylation, and hydrolysis when performing LC-MS analyses at room temperature and at 70°C.…”

Section: Resultscontrasting

confidence: 76%

Comparative studies of peak intensities and chromatographic separation of proteolytic digests, PTMs, and intact proteins obtained by nanoLC-ESI MS analysis at room and elevated temperatures

Moskovets

Ivanov

2016

Anal Bioanal Chem

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This work demonstrates that the chromatographic separation performed at highly stabilized elevated temperature results in significant improvements in sensitivity, quantitative accuracy, chromatographic resolution, and run-to-run reproducibility of nanoLC-MS analysis of complex peptides mixtures. A newly developed platform was shown to provide conditions for accurate temperature stabilization and temperature homogeneity when performing nanoLC-ESI MS analysis. We quantitatively assessed and compared the recovery of peptides and small proteins from nanoLC columns at room and elevated temperatures. We found that analyses performed at highly stabilized elevated temperatures led to improved detection sensitivity, reproducibility, and chromatographic resolution in reversed-phase LC separation of unmodified peptides (both hydrophilic and hydrophobic), post-translationally modified peptides (O-phosphorylated), and small intact proteins. The analytical benefits of elevated temperatures for qualitative and quantitative proteomic LC-MS profiling were demonstrated using mixtures of synthetic peptides, tryptic digests of mixtures of model proteins, and digested total lysates of isolated rat kidney mitochondria. The effect of elevated temperature on the ion suppression was also demonstrated. Graphical Abstract A fragment of overlaid LC retention time-m/z planar views demonstrates the improved separation performance in the analysis of a complex peptide mixture at elevated temperature. Retention time-m/z 2D peptide features detected at 60 °C (magenta) were matched and aligned with features detected at room temperature (green).

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Section: Resultscontrasting

confidence: 76%

Comparative studies of peak intensities and chromatographic separation of proteolytic digests, PTMs, and intact proteins obtained by nanoLC-ESI MS analysis at room and elevated temperatures

Moskovets

Ivanov

2016

Anal Bioanal Chem

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“…Nevertheless, we successfully employed this type of column for the elution of thalidomide using either a pure water or a water-organic mobile phase at a maximum temperature of 1858C [42]. The only problem we encountered in that study was that there was a steady increase in the pressure when the column was flushed with a pure water mobile phase for a period of more than 5 h.…”

Section: Plrp-smentioning

confidence: 99%

Temperature and pH‐stability of commercial stationary phases

Teutenberg¹,

Hollebekkers²,

Wiese

et al. 2009

J of Separation Science

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Original PaperTemperature and pH-stability of commercial stationary phasesIn this paper, the temperature and pH stability of silica-based RP stationary phases were investigated. Furthermore, nonsiliceous phases like a polymeric column based on polystyrene divinylbenzene and a polybutadiene coated zirconium dioxide column were also included. The columns were heated up to 1508C at dynamic conditions, which means that the eluent consisting of water and methanol (90:10, v/v) was continuously purged through the packed bed. After every 5 h, the columns were cooled down to room temperature and the efficiency was measured by injecting a test sample based on the Neue test. It could be shown that some stationary phases exhibited a very good temperature stability at the test conditions specified above.

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“…Teutenberg and coworkers [102] investigated the on-column stability of thalidomide using HTLC and found that not only temperature and residence time in the column have important influences on the analyte stability, but the column stationary phase also played a major role. Degradation of thalidomide occurred using carbon clad zirconium dioxide column, but no degradation occurred with a polystyrene divinylbenzene column using the same method conditions at 180 C.…”

Section: Pharmaceutical Applicationsmentioning

confidence: 99%

Developments in Fast Liquid Chromatographic Analysis of Pharmaceuticals

Natishan

2011

Journal of Liquid Chromatography & Related Technologies

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& The need for pharmaceutical analytical laboratories to increase sample throughput and speed of analysis to meet accelerated drug development timelines has led to considerable interest in the development of fast liquid chromatographic technology. Fast liquid chromatographic pharmaceutical analysis is accomplished by developments in ultra-fast high performance liquid chromatographic instrumentation, emerging technologies in column stationary phases and high temperature liquid chromatographic analysis. This review provides an overview and gives recent developments and applications in the different strategies used in fast liquid chromatographic pharmaceutical analysis.

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Influence of the stationary phase on the stability of thalidomide and comparison of different methods for the quantification of thalidomide in tablets using high-temperature liquid chromatography

Cited by 20 publications

References 44 publications

Comparative studies of peak intensities and chromatographic separation of proteolytic digests, PTMs, and intact proteins obtained by nanoLC-ESI MS analysis at room and elevated temperatures

Comparative studies of peak intensities and chromatographic separation of proteolytic digests, PTMs, and intact proteins obtained by nanoLC-ESI MS analysis at room and elevated temperatures

Temperature and pH‐stability of commercial stationary phases

Developments in Fast Liquid Chromatographic Analysis of Pharmaceuticals

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