Influence of temperature and nutritional requirements for mycelial growth of Eutypa lata, a vineyard pathogenic fungus

Amborabé, Bénigne-Ernest; Octave, Stéphane; Roblin, Gabriel

doi:10.1016/j.crvi.2005.01.006

Cited by 13 publications

(5 citation statements)

References 13 publications

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“…During the fungal attack, the degradation of lignin, cellulose, and hemicellulose leads to the formation of cavities in the walls and thus, makes the wood brittle [ 70 ]. In vitro studies highlighted the strong lysis activity of plant cells (leaves or cell suspension) by fungal filtrate proteins, including hydrolytic enzymes such as chitinases, β-1.3-glucanases, glycosidases, and xylanases [ 71 ]. Some studies also revealed the presence of toxic molecules secreted by the fungus under in vitro conditions, including acetylene and heterocyclic compounds.…”

Section: The Complexity Of Grapevine Trunk Diseasesmentioning

confidence: 99%

A Panoramic View on Grapevine Trunk Diseases Threats: Case of Eutypa Dieback, Botryosphaeria Dieback, and Esca Disease

Kenfaoui

Radouane

Mennani

et al. 2022

JoF

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Grapevine trunk diseases (GTD) are currently one of the most devastating and challenging diseases in viticulture, leading to considerable yield losses and a remarkable decline in grapevine quality. The identification of the causal agents is the cornerstone of an efficient approach to fighting against fungal diseases in a sustainable, non-chemical manner. This review attempts to describe and expose the symptoms of each pathology related to GTD, the modes of transmission, and the harmfulness of recently reported agents. Special attention was given to new diagnostic tests and technologies, grapevine defense mechanisms, molecular mechanisms of endophytes fungal colonization, and management strategies used to control these threats. The present extended review is, therefore, an updated state-of-the-art report on the progress in the management of vineyards.

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Section: The Complexity Of Grapevine Trunk Diseasesmentioning

confidence: 99%

A Panoramic View on Grapevine Trunk Diseases Threats: Case of Eutypa Dieback, Botryosphaeria Dieback, and Esca Disease

Kenfaoui

Radouane

Mennani

et al. 2022

JoF

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“…Harvesting was at the end of September, corresponding to the end of a cycle for vine: leaves and cordons are mature, and nutrients are stored in the xylem sink to face the winter period. Moreover, from the start of spring, the pathogen is exposed to optimal growth conditions, in particular temperature (Amborabé et al 2005). Samples were taken from the trunks of eight infected vines, in the brown and wedge-shaped area, in discoloured and apparently healthy tissue at the margin of the canker, and from the base and apex of annual canes.…”

Section: Plant Materialsmentioning

confidence: 99%

Structural modifications induced by Eutypa lata in the xylem of trunk and canes of Vitis vinifera

Rudelle

Octave

Kaïd-Harche

et al. 2005

Functional Plant Biol.

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Eutypa dieback, a devastating disease in grapevines, is caused by the fungal pathogen Eutypa lata, a wood-inhabiting fungus. E. lata acts by degrading wood tissues in the colonisation areas, and produces foliar symptoms. These striking symptoms have been attributed to the production of toxic metabolites by the pathogen, the most widely studied being eutypine. The aims of the study were to compare the effects of E. lata on xylem structure at the site of infection and in remote tissues. In healthy Vitis vinifera, the vessel-associated cells (VACs) in the trunk have a protective layer that covers the entire lignified wall and forms a transfer apparatus in pits located at the VAC / vessel interface. This apparatus occurs similarly in VACs in the basal part of canes but is less developed in the apical part. In the presence of E. lata, which is found only in the trunk and the cordons, the VACs initiated a program of secretory activity that led to the enlargement of the transfer apparatus, which is formed by tightly associated fibrils. This secretory activity was followed by VAC death. Furthermore, the hypertrophy of the transfer apparatus spread according to an acropetal gradient in the canes. Treatment with eutypine also induced the development of the transfer apparatus in VACs of basal and apical parts of canes excised from healthy vines. However, this apparatus was formed by loosely packed fibrils in VACs that were not completely damaged. Therefore, metabolites other than eutypine are expected to be involved in the VAC degeneration observed in infected vines.

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“…Mycelium was grown on a sterile medium containing yeast nitrogen base (YNB W/O amino acids and ammonium sulphate, Difco 233520, Detroit) at 1.7 g l −1 supplemented with glucose (10 g l −1 ) as carbon source and proline (5 g l −1 ) as nitrogen source (Amborabé et al 2005). Culture was initiated in 250 ml medium by addition of a mycelium disc (1 cm diameter) taken in a culture made on the same medium solidified with agar at 20 g l −1 .…”

Section: Methodsmentioning

confidence: 99%

Modifications of plant cell activities by polypeptides secreted by Eutypa lata, a vineyard fungal pathogen

Octave

Amborabé

Fleurat‐Lessard

et al. 2006

Physiologia Plantarum

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Eutypa dieback is a devastating disease induced in vineyards by the fungal pathogen Eutypa lata. The fungus colonizes the xylem tissues of trunk and cordons but is never found in the annual canes. Nevertheless, dwarfed shoots and leaf necrosis observed in diseased plants indicate that a necrotic signal can spread at a distance from the infected area. Eutypine, a small cyclic molecule, and related compounds have been postulated as the toxins inducing these symptoms. In this work, we evidence that E. lata secreted other metabolites of polypeptidic nature which induced toxic effects on canes and leaves of vines, and on leaves of other plant materials. The polypeptide fraction (PF) isolated from culture medium of mycelium induced transitory Hþ fluxes and membrane depolarization of plant cells. Complementary assays with plasma membrane vesicles (PMV) showed that Hþ-ATPase is a primary site of action as indicated by inhibition of the enzyme activity and increase of Hþ conductance of plasma membrane. The toxic effect was also obvious on respiration and photosynthesis. All these impairments led to a hindering in cell energetics and, as a consequence, to an inhibition of uptake of assimilates. Treatment with PF also triggered biological events, characteristics of elicitation as suggested by the early responses on cell membrane described above, the activation of NADPH oxidase and the activation of Phenylalanine ammonia lyase (PAL)

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Influence of temperature and nutritional requirements for mycelial growth of Eutypa lata, a vineyard pathogenic fungus

Cited by 13 publications

References 13 publications

A Panoramic View on Grapevine Trunk Diseases Threats: Case of Eutypa Dieback, Botryosphaeria Dieback, and Esca Disease

A Panoramic View on Grapevine Trunk Diseases Threats: Case of Eutypa Dieback, Botryosphaeria Dieback, and Esca Disease

Structural modifications induced by Eutypa lata in the xylem of trunk and canes of Vitis vinifera

Modifications of plant cell activities by polypeptides secreted by Eutypa lata, a vineyard fungal pathogen

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