A Panoramic View on Grapevine Trunk Diseases Threats: Case of Eutypa Dieback, Botryosphaeria Dieback, and Esca Disease

Kenfaoui, Jihane; Radouane, Nabil; Mennani, Mohammed; Tahiri, Abdessalem; Ghadraoui, Lahsen El; Belabess, Zineb; Fontaine, Florence; Hamss, Hajar El; Amiri, Saïd; Lahlali, Rachid; Barka, Essaïd Ait

doi:10.3390/jof8060595

Cited by 38 publications

(36 citation statements)

References 172 publications

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“…Esca includes trunk necrosis development in mature vine, along with foliar symptoms and/or symptoms on the shoots with grape wilting. The expression of foliar symptoms can be discontinuous, but plants usually die within a few years following the onset of initial symptoms (Bruez et al ., 2013; Kenfaoui et al ., 2022). The discontinuous expression of the disease suggests complex interactions with potential pathogenic species and environmental conditions (Andolfi et al ., 2011).…”

Section: Introductionmentioning

confidence: 99%

Landscape-scale endophytic community analyses in replicated grapevine stands reveal that dieback disease is not caused by specific fungal communities

Monod,

Hofstetter,

Viret

et al. 2024

Preprint

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Tree diebacks are complex and multi-factorial diseases with suspected biotic and abiotic components. Microbiome effects on plant health are challenging to assess due to the complexity of fungal and bacterial communities. Grapevine wood dieback is the main threat to sustainable production worldwide and no causality with microbial species has been established. Here, we aimed to test the hypothesis that grapevine esca disease progression has reproducible drivers in the fungal species community. For this, we analyzed a set of 21 vineyards planted simultaneously with a single susceptible cultivar to provide replication at the landscape scale. We sampled a total of 496 plants across vineyards in two different years to perform deep amplicon sequencing analyses of the fungal communities inhabiting grapevine trunks. The communities were highly diverse with a total of 4,129 amplified sequence variants assigned to 697 distinct species. Individual plants varied in fungal community composition depending on the year of sampling, vineyard location, and disease status. However, we detect no specific fungal species driving symptom development across the vineyards contrary to long-standing expectations. Our study shows how landscape-scale replicated field surveys allow for powerful hypothesis-testing for complex dieback disease drivers and prioritize future research towards additional factors.

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Section: Introductionmentioning

confidence: 99%

Landscape-scale endophytic community analyses in replicated grapevine stands reveal that dieback disease is not caused by specific fungal communities

Monod,

Hofstetter,

Viret

et al. 2024

Preprint

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“…Grapevine trunk diseases (GTDs) caused by fungal pathogens are responsible for significant harvest decrease and economic loss in viticultural areas around the globe (Cortesi et al, 2000; Fontaine et al, 2016; Kenfaoui et al, 2022; Ouadi et al, 2019) with Esca disease representing one of the most devastating, along with Botryosphaeria and Eutypa dieback. Esca can be considered a disease‐complex involving fungal species of several genera, with ascomycetes like Phaeomoniella chlamydospora (PCH) (Crouse & Gams, 2000) and Phaeoacremonium minimum (PMI) (Crous et al, 1996), formerly Phaeoacremonium aleophilum , as well as the basidiomycete Fomitiporia mediterranea (FMED) (Fischer, 2002) being regarded some of the most important causal agents (Fischer & Kassemeyer, 2003).…”

Section: Introductionmentioning

confidence: 99%

Detection of Esca‐associated fungi in grapevine trunks using loop‐mediated isothermal amplification (LAMP) assays

Marek,

Eberl,

Otba

et al. 2023

Annals of Applied Biology

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Esca is a grapevine trunk disease (GTD) that is caused by filamentous fungi. It is responsible for considerable economic losses in viniculture on a global scale. Despite many unknown factors contributing to the development of symptoms in affected plants, Phaeoacremonium minimum (PMI), Phaeomoniella chlamydospora (PCH), and Fomitiporia mediterranea (FMED) are generally considered as the main causative fungal species. Early detection and specific identification of these pathogens therefore play an important role in disease control and evaluation of suitable countermeasures. In this study, loop‐mediated isothermal amplification (LAMP) assays were developed for each of the three pathogens. A genome‐based approach was applied for detection and selection of unique target DNA sequences. The designed primer sets showed overall good specificities, with some observed cross‐reactions towards closely related Phaeoacremonium species for the PMI primer set. The developed assays had detection limits of 100 pg (FMED, PMI) and 1 pg (PCH) per reaction (corresponding to 1,460 (FMED); 1,950 (PMI); 342 (PCH) genome copies per reaction). The application of the assays to field samples was demonstrated by testing individual infected grapevine trunks from two European viticultural regions using crude DNA obtained in a rapid sample preparation step. LAMP assay results matched those of PCR following a conventional DNA extraction protocol. The study showed that LAMP‐based rapid molecular detection of major Esca agents can serve as a useful tool for further research and surveillance of a highly devastating grapevine disease.The application of computer‐based whole genome comparison between target and non‐target species for the identification of unique target sequences as the basis for LAMP (or PCR) primer design was demonstrated to be a useful approach in species for which scarce sequence information is available. Moreover, the developed method for rapid DNA preparation from grapevine trunks may potentially be adapted to the DNA‐based detection also of other fungal species that cause grapevine trunk diseases.This article is protected by copyright. All rights reserved.

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“…Grapevine trunk diseases (GTDs) are the leading cause of vineyard deterioration, causing a decrease in the viability of vineyards, an increase in production costs, and major economic losses in the wine industry [1][2][3][4][5][6]. The three main GTDs are esca disease, Eutypa dieback, and Botryosphaeria dieback (BD), which generally attack the structural parts of plants [2,[6][7][8][9][10]. BD is present on all continents where wine or table grapes are grown [2,5,7,[11][12][13][14][15][16].…”

Section: Introductionmentioning

confidence: 99%

Higher virulence of Diplodia seriata on vines of cv. Cabernet Sauvignon associated with 10-year-old wood compared to young tissue

Larach¹,

Vega-Celedón²,

Salgado³

et al. 2023

Preprint

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In vineyards, Botryosphaeria dieback (BD) occurs in young and old plants. In the field, the prevalence and severity of the disease increase proportionally with the age of the vineyards. Among the pathogens that cause (BD), Diplodia seriata is the most prevalent species in Chile and other countries with a Mediterranean climate. To date, no information is available on the sus-ceptibility of adult wood to infection by this pathogen since most of the pathogenicity tests have been carried out on 1- or 2-year-old shoots or detached canes. Therefore, a pathogenicity test was carried out on plants under field conditions, with inoculations in plants, on one-year-old shoots, and 2- and 10-year-old wood in grapevine cv. Cabernet Sauvignon. A pathogenicity test was carried out with two isolates of D. seriata selected in an assay in detached canes that were 2-year-old. The results on plants showed that D. seriata was significantly more aggressive on 10-year-old than on one- or two-year-old tissue. These results were compared with the lesions ob-tained from two-year-old canes. The results of this work are consistent with the damage ob-served in the field, highlight the importance of identifying each pathogenic species that causes (BD) and contribute to the knowledge of the epidemiology of this disease in Mediterranean cli-mates.

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A Panoramic View on Grapevine Trunk Diseases Threats: Case of Eutypa Dieback, Botryosphaeria Dieback, and Esca Disease

Cited by 38 publications

References 172 publications

Landscape-scale endophytic community analyses in replicated grapevine stands reveal that dieback disease is not caused by specific fungal communities

Landscape-scale endophytic community analyses in replicated grapevine stands reveal that dieback disease is not caused by specific fungal communities

Detection of Esca‐associated fungi in grapevine trunks using loop‐mediated isothermal amplification (LAMP) assays

Higher virulence of Diplodia seriata on vines of cv. Cabernet Sauvignon associated with 10-year-old wood compared to young tissue

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