Modifications of plant cell activities by polypeptides secreted by <i>Eutypa lata</i>, a vineyard fungal pathogen

Octave, Stéphane; Amborabé, Bénigne-Ernest; Fleurat‐Lessard, Pierrette; Bergès, Thierry; Roblin, Gabriel

doi:10.1111/j.1399-3054.2006.00715.x

Cited by 19 publications

(19 citation statements)

References 34 publications

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“…The toxicity of the high molecular weight hydrophilic compounds produced by several fungi, identified as exopolysaccharides, has been reviewed by Evidente and Motta (2001). Indeed, work on eutypa dieback disease has shown that polypeptides secreted by E. lata participated in cell structure damage in grapevine (Octave et al 2006). The toxic effect of the same type of compound secreted by Pal and Pch has been shown in the case of esca disease on grapevine (Luini et al 2010).…”

Section: Discussionmentioning

confidence: 99%

Extracellular compounds produced by fungi associated with Botryosphaeria dieback induce differential defence gene expression patterns and necrosis in Vitis vinifera cv. Chardonnay cells

et al. 2014

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Three major grapevine trunk diseases, esca, botryosphaeria dieback and eutypa dieback, pose important economic problems for vineyards worldwide, and currently, no efficient treatment is available to control these diseases. The different fungi associated with grapevine trunk diseases can be isolated in the necrotic wood, but not in the symptomatic leaves. Other factors seem to be responsible for the foliar symptoms and may represent the link between wood and foliar symptoms. One hypothesis is that the extracellular compounds produced by the fungi associated with grapevine trunk diseases are responsible for pathogenicity.In the present work, we used Vitis vinifera cv. Chardonnay cells to test the aggressiveness of total extracellular compounds produced by Diplodia seriata and Neofusicoccum parvum, two causal agents associated with botryosphaeria dieback. Additionally, the toxicity of purified mellein, a characteristic toxin present in the extracellular compounds of Botryosphaeriaceae, was assessed.Our results show that the total extracellular compounds produced by N. parvum induce more necrosis on Chardonnay calli and induce a different defence gene expression pattern than those of D. seriata. Mellein was produced by both fungi in amounts proportional to its aggressiveness. However, when purified mellein was added to the culture medium of calli, only a delayed necrosis and a lower-level expression of defence genes were observed. Extracellular compounds seem to be involved in the pathogenicity of the fungi associated with botryosphaeria dieback. However, the doses of mellein used in this study are 100 times higher than those found in the liquid fungal cultures: therefore, the possible function of this toxin is discussed.

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Section: Discussionmentioning

confidence: 99%

Extracellular compounds produced by fungi associated with Botryosphaeria dieback induce differential defence gene expression patterns and necrosis in Vitis vinifera cv. Chardonnay cells

et al. 2014

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“…These proteins are involved in cell structure damage and affect grapevine metabolism (Octave et al 2006b and. In addition, the toxicity of proteins secreted by Pa. chlamydospora (Pch) and Pm.…”

Section: Introductionmentioning

confidence: 99%

Toxicity of extracellular proteins from Diplodia seriata and Neofusicoccum parvum involved in grapevine Botryosphaeria dieback

Bénard-Gellon¹,

Farine²,

Goddard³

et al. 2014

Protoplasma

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Botryosphaeria dieback, esca and Eutypa dieback are three economic major grapevine trunk diseases that cause severe yield reduction in vineyards worldwide. The frequency of disease symptoms has increased considerably over the past decade, and no efficient treatment is currently available to control these diseases. The different fungi associated with grapevine trunk diseases mainly induce necrotic wood and characteristic foliar symptoms. In this context, fungi virulence factors and host invasion are not well understood. We hypothesise that extracellular proteins produced by Diplodia seriata and Neofusicoccum parvum, two causal agents associated with Botryosphaeria dieback, are virulence factors responsible for the pathogenicity. In our previous work, we demonstrated that the total extracellular compounds produced by N. parvum induced more necrosis on Chardonnay calli and triggered a different defence gene expression pattern than those produced by D. seriata. Furthermore, this aggressiveness was not clearly correlated with the production of mellein, a characteristic phytotoxin of Botryosphaeriaceae, in our in vitro calli model. To characterise other potential virulence factors and to understand the mechanisms of host invasion by the fungus, we evaluated the profile, quantity and the impact of extracellular proteins produced by these fungi on Vitis vinifera calli necrosis and defence gene expression. Our results reveal that, under the same conditions, N. parvum produces more extracellular proteins and in higher concentrations than D. seriata. With Vitis vinifera cv. Chardonnay cells, we showed that equivalent concentrations of proteins secreted by N. parvum were more aggressive than those of D. seriata in producing necrosis and that they clearly induced more grapevine defence genes.

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“…Antibodies have been raised against E. lata ascospores and hyphal material and used to identify the fungus by immunodiffusion and immunofluorescence (Francki & Carter, ). More recently, it has been shown that E. lata secretes various polypeptides (Octave et al ., ,b) and enzymes (Schmidt et al ., ) into its culture medium. Rabbit antibodies raised against these polypeptides were used to develop a sensitive and specific serological assay for the in planta identification of E .…”

Section: Discussionmentioning

confidence: 99%

Identification of grapevine marker genes for early, non‐destructive Eutypa lata infection diagnosis

et al. 2013

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Eutypa lata is the causal agent of eutypa dieback, a highly damaging trunk disease affecting all grape‐growing areas, with currently neither an efficient curative treatment nor an early non‐destructive diagnostic method. The present work was carried out to discover grapevine genes expressed in response to the presence of E. lata that could be useful to develop an early (before visible foliar symptoms) and non‐destructive (using grapevine leaves) diagnostic tool. Microarray analyses were carried out from (i) infected plants showing characteristic E. lata foliar and vascular symptoms and positive pathogen recovery from vascular lesions (S+R+), (ii) infected plants showing no symptoms (S−R+), and (iii) symptomless plants with negative pathogen recovery (S−R−). Vineyard and greenhouse‐grown plants, naturally or artificially infected respectively, and uninoculated controls were characterized and leaf RNA was hybridized with 15k operon grapevine oligonucleotide microarrays. Among the grapevine genes differentially expressed between S−R+ and S−R− plants in greenhouse and vineyard conditions, 10 were highlighted as robust candidate genes for diagnosis: seven were specifically involved in response to infection and three were associated with symptom absence. Five were confirmed to be effective diagnostic marker genes usable in a qRT‐PCR‐based test performed on RNA extracted from grapevine leaves cultivated in either greenhouse or vineyard conditions. Furthermore, their expression profiles in response to infection with E. lata or other major grapevine fungi (Erysiphe necator, Plasmopara viticola, Botrytis cinerea) could be distinguished. The usefulness of these genes to develop an early and non‐destructive method for diagnosis of E. lata infection is discussed with regard to the advantages and drawbacks of previous E. lata diagnostic studies.

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Modifications of plant cell activities by polypeptides secreted by Eutypa lata, a vineyard fungal pathogen

Cited by 19 publications

References 34 publications

Extracellular compounds produced by fungi associated with Botryosphaeria dieback induce differential defence gene expression patterns and necrosis in Vitis vinifera cv. Chardonnay cells

Extracellular compounds produced by fungi associated with Botryosphaeria dieback induce differential defence gene expression patterns and necrosis in Vitis vinifera cv. Chardonnay cells

Toxicity of extracellular proteins from Diplodia seriata and Neofusicoccum parvum involved in grapevine Botryosphaeria dieback

Identification of grapevine marker genes for early, non‐destructive Eutypa lata infection diagnosis

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