Influence of streptozotocin and alloxan induced diabetes on the metabolism of dermal collagen in albino rats

Abstract

The metabolism of collagen in male rats made diabetic by treatment with either streptozotocin or alloxan was studied after the injection of 3H-proline by estimating specific and total 3H-hydroxyproline activity in skin collagen fractions and urine. Experimentally induced diabetes was found to decrease the neutral salt-soluble and acid-soluble collagen with no change in insoluble collagen as compared to a control group. The specific and total radioactivity of 3H-hydroxyproline in soluble and insoluble collagen fractions were also decreased. Studies of total 3H-hydroxyproline activities in soluble collagens and insoluble collagen showed that the conversion of soluble to insoluble collagen was influenced by diabetes. Both streptozotocin and alloxan were found to increase urinary excretion of total hydroxyproline and 3H-hydroxyproline during the first 12 h after the administration of 3H-proline. Weekly analyses of urinary hydroxyproline also indicated a similar pattern. The results of the present investigation clearly indicate decreased synthesis and increased catabolism of collagen accompanied by accelerated conversion of soluble to insoluble collagen in experimentally induced diabetes.

Influence of hyperbaric oxygen therapy on peri‐implant bone healing in rats with alloxan‐induced diabetes

“…) occurs along with decreased synthesis of both collagen (Spanheimer , Schneir et al. ) and proteoglycan (Mohanam & Bose , Ramamurthy & Golub , Weiss et al. ).…”

Influence of streptozotocin- and alloxan-induced diabetes in the rat on collagenase and certain lysosomal enzymes in relation to the degradation of connective tissue proteins

“…Golub et al [15] observed that both alloxan-and streptozotocin-induced diabetes stimulated the collagenolytic activity in gingiva in tissue culture. In addition, the diabetic rats exhibited increased urinary excretion of hydroxyproline [24]. The pathophysiological role of the inhibition of collagenolytic systems in the kidney of streptozotocin-diabetic rats is unknown.…”

“…Four-month-old male albino rats were divided into three groups: control animals, streptozotocin-treated and alloxan-treated. Rats were starved for 24 h and given an IP injection of alloxan in 0.154 tool/1 NaC1 (20 mg/100 g body weight) or an IV injection of streptozotocin (Upjohn, Kalamazoo, Michigan, USA) in isotonic saline containing 1 mol/1 citric acid (pH 4.5) (6.5 mg/100 g body weight) [24]. All animals received commercial rat feed (Hindustan Lever, Bombay) and water ad libitum.…”

“…Body weights were recorded at weekly intervals. Blood samples were taken weekly from the tails of fasted rats for measurement of glucose [24]. At the end of the experimental period (3 weeks), 12 rats in each group were killed by decapitation and the skin, liver, kidney and spleen were removed immediately in ice-cold containers and stored at -20 ~ Blood was collected by heart puncture before killing the rats and allowed to clot after standing at room temperature for 2-4 h. The serum was separated by centrifugation at low speed and stored at -20 ~ until assayed.…”