Influence of streptozotocin- and alloxan-induced diabetes on the crosslinking of dermal collagen

Abstract

The influence of experimentally induced diabetes on rat dermal collagen crosslinking was investigated in male albino rats. In comparison to the normal, the diabetic group demonstrated decrease in percent reversibility of neutral salt-soluble collagen gel and susceptibility of insoluble collagen to denaturing agents and pronase whereas the aldehyde content was significantly increased. The electrophoretic gels revealed a marked decrease of alpha/beta ratio in diabetic animals. The results indicated that both the intra- and intermolecular crosslinkings of collagen were increased in experimentally induced diabetes.

Influence of streptozotocin- and alloxan-induced diabetes in the rat on collagenase and certain lysosomal enzymes in relation to the degradation of connective tissue proteins

“…The collagen preparations were freeze-dried and stored at -20 ~ until use. The specific radioactivity was found to be in the range of 2 500 cpm/mg of collagen and the purity of the collagen was confirmed by disc electrophoresis in 5% polyacrylamide gels containing sodium dodecyl sulphate [25].…”

“…Six hours later the animals were anaesthetized with ether and killed by decapitation. The skins were shaved, minced with NaC1 (0.5 tool/l) in the cold and the neutral salt soluble collagen was extracted and purified as described previously [25]. The collagen preparations were freeze-dried and stored at -20 ~ until use.…”