Influence of Solid Media on Growth of Mycelia and Antibacterial Activity of Wild Macrofungi, Macrocybe Gigantea

Abstract

The present study was focused on wild pantropical macrofungi, Macrocybe gigantea (Massee) Pegler & Lodge, collected from Agumbe rain forests of Karnataka, India. The macrofungal tissues was subjected to mycelial culturing in four different solid nutritive media viz., Potato Dextrose Agar, Malt Extract Agar, Sabouraud's Dextrose Agar and Czapek Dextrose Agar. The study also evaluated the invitro antibacterial activity of ethyl acetate extract of mycelial filtrates obtained from Potato Dextrose Broth cultures of M. gigantea. The crude extracts were tested against five Gram-negative bacteria using agar well diffusion assay. Our data showed that among the soild media tested, the mycelium grown in Potato Dextrose Agar recorded the maximum growth followed by Malt Extract Agar. The crude extracts had inhibition against Salmonella typhi, Escherihia coli, Klebsiella pneumoniae, Pseudomonas syringae and Xanthomonas campestris. The maximum inhibition was against S.typhi. The results revealed that the extracts had the potent bioactive compounds with antibacterial property.

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“…Our results were similar with Shim et al [ 32 ] in which they found PDA medium as the suitable medium for the cultural characteristics of Cystoderma amianthinum. These results were in agreement with work of Roy & Krishnappa [ 33 ]. They used PDA, SDA, MEA and czapek dextrose agar media (CZA) for mycelial growth of Macrocybe gigantea and found PDA as the best medium for maximum mycelial growth of M. gigantea .…”